Nanofabricated photon tunneling based sensor

• US 7,267,797 B1
• Filed: 11/07/2001
• Issued: 09/11/2007
• Est. Priority Date: 11/14/2000
• Status: Expired due to Fees

• Current Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Original Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Inventors: Craighead, Harold G., Kameoka, Jun
• Primary Examiner(s): SINES, BRIAN J
• Application Number:
  US10/010,939
• Time in Patent Office: 2,134 Days
• Field of Search: 422/50, 422/52, 422/55, 422/58, 422/68.1, 422/82.05, 422/82.06, 422/82.07, 422/82.08, 422/82.09, 422/82.11, 436/164, 436/172, 436/63, 73/10.2, 977/920, 977/953, 977/957, 977/958, 977/834
• US Class Current: 422/82.05
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 21/0303 Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment G01N21/031 - G01N21/15 take precedence
• CPC Class Codes: G01N 21/4133 Refractometers, e.g. differential
• CPC Class Codes: Y10S 977/834 Optical properties of nanomaterial, e.g. specified transparency, opacity, or index of refraction
• CPC Class Codes: Y10S 977/92 Detection of biochemical
• CPC Class Codes: Y10S 977/953 Detector using nanostructure
• CPC Class Codes: Y10S 977/957 Of chemical property or presence
• CPC Class Codes: Y10S 977/958 Of biomolecule property
• CPC Class Codes: Y10T 436/11 Automated chemical analysis

Abstract

A system and method for detecting changes in the refractive index of a fluid in a small test volume. A change in the refractive index can indicate a change in the chemical composition of the fluid. The test volume has a depth comparable to or less than the wavelength of incident light. In one embodiment, an internal surface of the volume is coated with a binding partner selected to bind with a targeted molecule. When the targeted molecule binds to the binding partner, the optical properties of the system change. The refractive index is determined by illuminating the test volume with laser light and measuring transmitted or reflected light.

Claims

1. A refractive index detector comprising:

a duct, said duct having an interior surface and a substantially parallel gap formed by a first wall and a second wall of said duct, said first wall and said second wall are transparent, said duct configured to receive a solution and configured to reflectively communicate light to an optical sensor as a function of said solution in said duct and as a function of light, having a wavelength, incident to said detector at an angle greater than a critical angle, and wherein a refractive index is calculated based on reflected light as measured by said optical sensor.

2. The detector of claim 1 wherein said gap is approximately equal to or less than the wavelength of said light.

3. The detector of claim 1 wherein said first wall and said second wall are translucent.

4. The detector of claim 1 wherein said duct comprises glass.

5. The detector of claim 1 wherein said gap has a cross sectional dimension of between 50 and 1000 nanometers.

6. The detector of claim 1 wherein said duct includes a binding partner for an analyte immobilized on at least a portion of said interior surface of said duct, said binding partner capable of binding to said analyte.

7. The detector of claim 6 wherein said duct has a refractive index greater than a refractive index of said binding partner.

8. The detector of claim 6 wherein said analyte comprises a pathogen, a microorganism, a bacteria, or a virus.

9. The detector of claim 6 wherein said binding partner for said analyte is an antibody or antibody fragment that binds said analyte.

10. The detector of claim 6 wherein said analyte is a ligand specific for a cellular receptor and said binding partner is a cellular receptor.

11. The detector of claim 6 wherein said binding partner is a ligand for a cellular receptor and said analyte is a cellular receptor.

12. The detector of claim 6 wherein said analyte is a metallic ion and said binding partner is a chelator that binds said metallic ion.