Nucleic acids encoding recombinant proteins containing Shiga-like toxin and vascular endothelial growth factor
First Claim
1. An isolated nucleic acid encoding a fusion protein comprising:
- (1) the A subunit of Shiga-like bacterial toxin, said subunit having the nucleic acid sequence of SEQ ID NO;
9; and
(2) human vascular endothelial growth factor, said growth factor having the nucleic acid sequence of SEQ ID NO;
10;
wherein said fusion protein possesses ribosome inactivating activity.
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Abstract
The present invention is directed to an isolated nucleic acid encoding a fusion protein comprising (1) the A subunit of Shiga-like bacterial toxin, or a truncated or mutated version thereof; and (2) human vascular endothelial growth factor, or a truncated or mutated version thereof; wherein the fusion protein possesses ribosome inactivating activity and ability to bind to cellular VEGF receptors. The present invention is also directed to polypeptides the above combination of toxin and growth factor, as well as expression vectors and transformed cells incorporating the above nucleic acid. The invention is also directed to pharmaceutical compositions and methods for treating patients suffering from diseases relating to angiogenesis.
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Citations
11 Claims
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1. An isolated nucleic acid encoding a fusion protein comprising:
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(1) the A subunit of Shiga-like bacterial toxin, said subunit having the nucleic acid sequence of SEQ ID NO;
9; and(2) human vascular endothelial growth factor, said growth factor having the nucleic acid sequence of SEQ ID NO;
10;wherein said fusion protein possesses ribosome inactivating activity. - View Dependent Claims (2, 3, 4, 5)
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6. An expression vector, comprising:
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(1) a nucleic acid encoding a fusion protein comprising the A subunit of Shiga-like bacterial toxin; and
human vascular endothelial growth factor; and(2) a promoter sequence operably linked to said nucleic acid to allow expression of said nucleic acid; said expression vector comprising the nucleic acid sequence of SEQ ID NO;
11. - View Dependent Claims (7, 8, 9)
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10. An isolated nucleic acid comprising SEQ ID NO:
- 9 and SEQ ID NO;
11.
- 9 and SEQ ID NO;
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11. An isolated nucleic acid comprising the nucleic acid sequence of SEQ ID NO:
- 11.
Specification