Fluorescence correlation spectroscopy instrument and method of using the same
First Claim
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1. A portable fluorescence correlation spectroscopy instrument comprising:
- a sample flow chamber;
an excitation source;
a first dichromatic mirror positioned to reflect light from said excitation source;
at least one light focusing element positioned to receive light emitted by said excitation source and transmitted through said first dichromatic mirror;
a detector for detecting light, said detector positioned to receive light emitted by a sample excited by said excitation source; and
a correlator coupled to said detector, said correlator for processing data received at said detector and providing data comprising autocorrelation data, crosscorrelation data, or a combination thereof,the instrument being free of a microscope eyepiece.
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Abstract
Disclosed is a portable fluorescence correlation spectroscopy instrument that includes an excitation source, at least one of a light focusing element positioned to receive light emitted by the excitation source, a detector for detecting light, the detector positioned to receive light emitted by a sample excited by the excitation source, and a correlator coupled to the detector, the correlator for processing data received at the detector and providing data including autocorrelation data, crosscorrelation data, or a combination thereof.
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Citations
66 Claims
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1. A portable fluorescence correlation spectroscopy instrument comprising:
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a sample flow chamber; an excitation source; a first dichromatic mirror positioned to reflect light from said excitation source; at least one light focusing element positioned to receive light emitted by said excitation source and transmitted through said first dichromatic mirror; a detector for detecting light, said detector positioned to receive light emitted by a sample excited by said excitation source; and a correlator coupled to said detector, said correlator for processing data received at said detector and providing data comprising autocorrelation data, crosscorrelation data, or a combination thereof, the instrument being free of a microscope eyepiece. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43)
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44. A portable fluorescence correlation spectroscopy instrument comprising:
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a chamber through which a liquid sample can flow, said chamber being positioned such that the confocal plane of said instrument is contained within said chamber; a monochromatic light source; a light focusing device adapted to focus light emitted by said monochromatic light source on a sample; a detector capable of detecting light; a fiber optic positioned to receive light emitted by a sample excited by said light source, said fiber optic being coupled to said detector; and a correlator coupled to said detector, said correlator being capable of processing data received at said detector and providing data comprising autocorrelation data, crosscorrelation data, or a combination thereof.
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45. A portable fluorescence correlation spectroscopy instrument comprising:
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a sample chamber; an excitation source; a first light focusing element comprising a fiber optic positioned to receive light emitted by said excitation source; a second light focusing element, said fiber optic having a first end disposed in said sample chamber, said second light focusing element being focused on the first end of said fiber optic; a detector for detecting light, said detector positioned to receive light emitted by a sample excited by said excitation source; and a correlator coupled to said detector, said correlator for processing data received at said detector and providing data comprising autocorrelation data, crosscorrelation data, or a combination thereof. - View Dependent Claims (46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64)
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65. A fluorescence correlation spectroscopy system comprising:
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a carrying case; and a portable fluorescence correlation spectroscopy instrument disposed in said carrying case and being operable in said carrying case, said instrument comprising a sample flow chamber, an excitation source, at least one light focusing element positioned to receive light emitted by said excitation source, a detector for detecting light, said detector positioned to receive light emitted by a sample excited by said excitation source, and a correlator coupled to said detector, said correlator for processing data received at said detector and providing data comprising autocorrelation data, crosscorrelation data, or a combination thereof.
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66. A portable fluorescence correlation spectroscopy instrument comprising:
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a sample flow chamber; an excitation source; at least one light focusing element positioned to receive light emitted by said excitation source; a detector for detecting light, said detector positioned to receive light emitted by a sample excited by said excitation source; an emission filter positioned to transmit light to said detector, said emission filter adapted to transmit light having a wavelength range greater than the range of wavelengths emitted by said excitation source; and a correlator coupled to said detector, said correlator for processing data received at said detector and providing data comprising autocorrelation data, crosscorrelation data, or a combination thereof, said instrument operating in a fixed stage mode.
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Specification