Automated molecular pathology apparatus having fixed slide platforms

• US 7,270,785 B1
• Filed: 10/30/2002
• Issued: 09/18/2007
• Est. Priority Date: 11/02/2001
• Status: Active Grant

• Current Assignee: Ventana Medical Systems Incorporated (Roche Holding AG)
• Original Assignee: Ventana Medical Systems Incorporated (Roche Holding AG)
• Inventors: Showalter, Wayne, Richards, William, Lemme, Charles D.
• Primary Examiner(s): Warden; Jill
• Assistant Examiner(s): Nagpaul; Jyoti
• Application Number:
  US10/283,639
• Time in Patent Office: 1,784 Days
• Field of Search: 422 99-101, 422 63- 65, 436/180, 436/46
• US Class Current: 422/64
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: Y10T 436/112499 with sample on test slide
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

Apparatus and methods for automatically staining or treating multiple tissue samples mounted on slides are provided, in which the slides and reagent bottles are held in fixed position, and the reagent and wash solutions brought to the slides.

Claims

1. An automatic slide processing apparatus comprising:

(a) a slide support tray slidably mounted on rails to permit loading and unloading of slides into and from the apparatus;

(b) a reagent delivery tray;

(c) a nozzle support plate concentrically mounted between said slide support tray and said reagent delivery tray; and

(d) a reagent dispensing mechanism rotatably and concentrically mounted above said reagent delivery tray for selectively dispensing reagent on a slide carried on the slide support tray;

wherein the slide support tray and the reagent delivery tray are operatively fixedly positioned in said apparatus, and the nozzle support plate is selectively rotatably operatively movable relative to the slide support tray and the reagent delivery tray, and wherein said slide support tray includes at least one liquid drain.

2. An apparatus as claimed in claim 1, wherein said slide support tray carries a plurality of radially spaced slide support platforms.

3. An apparatus according to claim 2, wherein said slide support platforms include heaters.

4. An apparatus according to claim 2, wherein said slide support platforms include temperature sensors.

5. An apparatus according to claim 1, and further including a damping mechanism for damping sliding movement on the rails.

6. An apparatus according to claim 1, wherein said rails are supported on a lift mechanism.

7. An apparatus according to claim 1, wherein said reagent dispensing mechanism is selectively moveable relative to the reagent delivery tray.

8. An apparatus according to claim 7, wherein said reagent dispensing mechanism comprises robotic dispensing pipettes.

9. An apparatus according to claim 8, wherein said dispensing pipettes are vertically moveable between an operating position and a transport position.

10. An apparatus according to claim 7, wherein said reagent dispensing mechanism is rotatably operatively movable over a range of 185°

plus or minus from a home position.

11. An apparatus according to claim 1, wherein said nozzle support plate carries at least one vortex air jet.

12. An apparatus according to claim 1, wherein said nozzle support plate carries at least one air knife.

13. An apparatus according to claim 1, wherein said nozzle support plate carries at least one applicator for applying a cover fluid to the slide.

14. An apparatus according to claim 1, wherein said nozzle support plate is rotatably operatively movable over a range of 185°

plus or minus from a home position.

15. An apparatus according to claim 1, and further comprising a reagent bar code reader carried with said reagent dispensing mechanism.

16. An apparatus according to claim 1, and further comprising a processor for controlling movement of said nozzle support plate and said reagent dispensing mechanism.

17. An apparatus according to claim 1, and further comprising a reagent dispensing mechanism wash station mounted on said nozzle support plate.

18. An apparatus according to claim 17, and comprising two reagent dispensing mechanism wash mechanisms mounted spaced from one another on said nozzle support plate.

19. An apparatus according to claim 1, wherein said slide support tray is operatively fixedly positioned relative to said reagent dispensing mechanism.

20. An automatic slide processing apparatus comprising:

(a) a slide support tray slidably mounted on rails to permit loading and unloading of slides into and from the apparatus;

(b) a reagent delivery tray;

(c) a nozzle support plate concentrically mounted between said slide support tray and said reagent delivery tray; and

(d) a reagent dispensing mechanism rotatably and concentrically mounted above said reagent delivery tray for selectively dispensing reagent on a slide carried on the slide support tray;

wherein the slide support tray and the reagent delivery tray are operatively fixedly positioned in said apparatus, and the nozzle support plate is selectively rotatably operatively movable relative to the slide support tray and the reagent delivery tray, and wherein said reagent dispensing mechanism comprises two spaced robotic dispensing pipettes which are selectively movable relative to the reagent delivery tray.

21. An automatic slide processing apparatus comprising:

(a) a slide support tray slidably mounted on rails to permit loading and unloading of slides into and from the apparatus;

(b) a reagent delivery tray;

(c) a nozzle support plate concentrically mounted between said slide support tray and said reagent delivery tray; and

(d) a reagent dispensing mechanism rotatably and concentrically mounted above said reagent delivery tray for selectively dispensing reagent on a slide carried on the slide support tray;

wherein the slide support tray and the reagent delivery tray are operatively fixedly positioned in said apparatus, and the nozzle support plate is selectively rotatably operatively movable relative to the slide support tray and the reagent delivery tray, and further comprising a slide bar code reader carried on said nozzle support plate.

22. An automatic slide processing apparatus comprising:

(a) a slide support zone slidably mounted to permit loading and unloading of slides into and from the apparatus;

(b) heating elements associated with the slide support zone for heating slides carried therein;

(c) a reagent support zone; and

(d) a nozzle support zone concentrically mounted between said slide support zone and said reagent support zone

(e) a reagent dispensing mechanism operatively disposed to directly access both said slide support zone and reagent support zone, wherein said slide support zone and said reagent support zone are operatively fixedly positioned in said apparatus, and said reagent dispensing mechanism is selectively rotatably mounted above said reagent support zone and operatively movable relative to said slide support zone and said reagent support zone, and wherein said slide support zone includes at least one liquid drain.

23. An apparatus as claimed in claim 22, wherein said slide support zone carries a plurality of radially spaced slide support platforms.

24. An apparatus according to claim 23, wherein said slide support platforms include heaters.

25. An apparatus according to claim 23, wherein said slide support platforms include temperature sensors.

26. An apparatus according to claim 22, and further including a damping mechanism for damping sliding movement on the rails.

27. An apparatus according to claim 22, wherein said rails are supported on a lift mechanism.

28. An apparatus according to claim 22, wherein said reagent dispensing mechanism is selectively moveable relative to the reagent support zone.

29. An apparatus according to claim 28, wherein said reagent dispensing mechanism comprises robotic dispensing pipettes.

30. An apparatus according to claim 29, wherein said dispensing pipettes are vertically moveable between an operating position and a transport position.

31. An apparatus according to claim 28, wherein said reagent dispensing mechanism comprises two spaced robotic dispensing pipettes.

32. An apparatus according to claim 28, wherein said reagent dispensing mechanism is rotatably operatively movable over a range of 185°

plus or minus from a home position.

33. An apparatus according to claim 22, and further including said nozzle support zone carrying at least one vortex air jet or at least one air knife above said slide support zone.

34. An apparatus according to claim 33, wherein said nozzle support zone carries at least one applicator for applying a cover fluid to the slide.

35. An apparatus according to claim 33, wherein said nozzle support zone is rotatably operatively movable over a range of 185°

plus or minus from a home position.

36. An apparatus according to claim 22, and further comprising a slide bar code reader carried with said nozzle support zone.

37. An automatic slide processing apparatus comprising:

(a) a slide support zone slidably mounted on rails to permit loading and unloading of slides into and from the apparatus;

(b) heating elements associated with the slide support zone for heating slides carried therein;

(c) a reagent support zone; and

(d) a nozzle support zone concentrically mounted between said slide support zone and said reagent support zone

(d) a reagent dispensing mechanism operatively disposed to directly access both said slide support zone and reagent support zone, wherein said slide support zone and said reagent support zone are operatively fixedly positioned in said apparatus, and said reagent dispensing mechanism is selectively rotatably mounted above said reagent support zone and operatively movable relative to said slide support zone and said reagent support zone and further comprising a reagent bar code reader carried with said reagent dispensing mechanism.

38. An apparatus according to claim 37, and further comprising said nozzle support zone and a processor for controlling movement of said nozzle support zone and said reagent dispensing mechanism.

39. An apparatus according to claim 37, and further comprising a nozzle support zone and a reagent dispensing mechanism wash station carried with said nozzle support zone.

40. An apparatus according to claim 39, and comprising a nozzle support zone and two reagent dispensing mechanism wash stations spaced from one another carried on said nozzle support zone.

41. An apparatus according to claim 37, wherein said slide support zone is operatively fixedly positioned relative to said reagent dispensing mechanism.