Methods and systems for processing microfluidic samples of particle containing fluids
First Claim
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1. A microfluidic device, comprising:
- an enrichment module configured to receive a microdroplet of particle-containing fluid, the enrichment module comprising a flow-through member and an enrichment chamber, wherein the flow-through member is configured to allow fluid of the particle-containing fluid to pass through the flow-through member thereby accumulating an enriched particle sample, comprising particles of the particle-containing fluid, in the enrichment chamber;
a downstream channel leading downstream from the enrichment chamber to at least one downstream processing module;
an actuator configured to move the enriched particle sample downstream from the enrichment module along the downstream channel; and
a first valve disposed between the actuator and the flow-through member.
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Abstract
The present invention relates to a microfluidic system for processing a cell-containing liquid. The system includes a microfabricated substrate having an enrichment channel to prepare an enriched cell sample from the cell-containing liquid. A flow through member is in liquid communication with the enrichment zone. The flow through member substantially prevents cells of the cell-containing fluid from exiting the enrichment zone while allowing liquid of the cell-containing liquid to exit the enrichment zone. A gas actuator associated with the enrichment zone provides a gas pressure sufficient to move the enriched cell sample from the enrichment zone.
352 Citations
42 Claims
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1. A microfluidic device, comprising:
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an enrichment module configured to receive a microdroplet of particle-containing fluid, the enrichment module comprising a flow-through member and an enrichment chamber, wherein the flow-through member is configured to allow fluid of the particle-containing fluid to pass through the flow-through member thereby accumulating an enriched particle sample, comprising particles of the particle-containing fluid, in the enrichment chamber; a downstream channel leading downstream from the enrichment chamber to at least one downstream processing module; an actuator configured to move the enriched particle sample downstream from the enrichment module along the downstream channel; and a first valve disposed between the actuator and the flow-through member. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. A microfluidic device, comprising:
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an enrichment module configured to separate an enriched particle sample from a microdroplet of particle-containing fluid, wherein the enrichment module comprises a flow-through member and an enrichment chamber; a processing module disposed downstream of the enrichment module; an actuator configured to move the enriched particle sample downstream from the enrichment module with essentially no dilution of the enriched particle sample; and a first valve disposed between the actuator and the flow-through member. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42)
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Specification