Apparatus and method for adjustment of ion separation resolution in FAIMS

• US 7,274,014 B2
• Filed: 11/23/2005
• Issued: 09/25/2007
• Est. Priority Date: 11/24/2004
• Status: Expired due to Fees

• Current Assignee: Thermo Finnigan LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Thermo Finnigan LLC (Thermo Fisher Scientific Incorporated)
• Inventors: Guevremont, Roger, Kapron, James T., Guevremont, legal representative
• Primary Examiner(s): Nguyen; Kiet T.
• Application Number:
  US11/285,148
• Publication Number:
  US 20060237643A1
• Time in Patent Office: 671 Days
• Field of Search: 250/287,286,281,282
• US Class Current: 250/287
• CPC Class Codes: G01N 27/624 Differential mobility spectrometry [DMS]; Field asymmetric-waveform ion mobility spectrometry [FAIMS]

Abstract

An apparatus for controllably varying specificity of a FAIMS-based ion separation includes a FAIMS analyzer region defined between a first electrode surface and a second other electrode surface. The FAIMS analyzer region is in communication with an ionization source for providing a flow of ions including a known ion, and with an ion outlet for extracting ions including the known ion from the FAIMS analyzer region. The first and second electrode surfaces are for having an asymmetric waveform voltage applied thereacross for defining an average ion flow path for the known ion traversing therebetween. An actuator is provided for controllably moving the first electrode surface relative to the second electrode surface for controllably varying the length of the average ion flow path.

Claims

1. An apparatus for controllably varying specificity of a FAIMS-based ion separation, comprising:

a first electrode comprising a first electrode surface;

a second electrode comprising a second electrode surface, the second electrode spaced-apart from the first electrode along a first direction such that the second electrode surface faces the first electrode surface and overlaps therewith along a second direction that is normal to the first direction so as to define a FAIMS analyzer region therebetween, the FAIMS analyzer region having an ion inlet end and an ion outlet end, the first electrode and the second electrode movable one relative to the other along the second direction for varying a length of the FAIMS analyzer region between the ion inlet end and the ion outlet end; and

,

an actuator for controllably relatively moving the first electrode along the second direction relative to the second electrode, for varying the length of the FAIMS analyzer region between the ion inlet end and the ion outlet end.

2. An apparatus according to claim 1, wherein the actuator is for supporting controllable movement of the first electrode relative to the second electrode to a position in which the length of the FAIMS analyzer region is approximately zero.

3. An apparatus according to claim 1, wherein the actuator comprises a motor coupled to one of the first electrode and the second electrode via a linkage member.

4. An apparatus according to claim 1, wherein the actuator comprises a manually operable mechanism coupled to one of the first electrode and the second electrode.

5. An apparatus according to claim 1, wherein the first electrode and the second electrode comprise inner and outer generally cylindrical electrodes, respectively, disposed in a concentric arrangement relative to a longitudinal axis defined along the second direction.

6. An apparatus according to claim 5, comprising a second inner electrode longitudinally spaced apart from the first electrode and coaxial therewith, a gap between the first electrode and the second inner electrode defining an ion inlet adjacent the ion inlet end, and further comprising an ion outlet defined within a portion of the second electrode and adjacent the ion outlet end.

7. An apparatus according to claim 6, comprising an ionization source in fluid communication with the ion inlet end via the ion inlet.

8. An apparatus according to claim 7, wherein the actuator is for translating the first electrode relative to the second electrode.

9. An apparatus according to claim 8, wherein the actuator is for translating the second inner electrode synchronously with the first electrode, such that the gap between the first electrode and the second inner electrode is approximately constant during translation.

10. An apparatus according to claim 7, wherein the actuator is for translating the second electrode relative to the first electrode.

11. An apparatus for controllably varying specificity of a FAIMS-based ion separation, comprising:

a first generally cylindrical inner electrode having a first length;

a second generally cylindrical inner electrode having a second length, the second generally cylindrical inner electrode axially aligned with and longitudinally spaced-apart from the first generally cylindrical inner electrode so as to define a circumferential gap between facing ends thereof, the circumferential gap defining an ion inlet;

an outer generally cylindrical electrode having a third length and disposed in a concentric overlapping arrangement with the first generally cylindrical inner electrode and with the second generally cylindrical inner electrode, the outer generally cylindrical electrode defining an ion outlet within a portion thereof, a space between the second generally cylindrical inner electrode and the outer generally cylindrical electrode defining a FAIMS analyzer region, an average ion flow path being defined through said FAIMS analyzer region between the ion inlet and the ion outlet; and

,

an actuator for relatively translating the outer generally cylindrical electrode relative to both the first generally cylindrical inner electrode and the second generally cylindrical inner electrode, so as to vary a length of the average ion flow path between the ion inlet and the ion outlet.

12. An apparatus according to claim 11, comprising an ionization source in fluid communication with the FAIMS analyzer region via the ion inlet.

13. An apparatus according to claim 12, wherein the ionization source is at least partially contained within the first generally cylindrical inner electrode.

14. An apparatus according to claim 11, comprising an electrical controller operatively coupled to the second generally cylindrical inner electrode for establishing an electric field between the second generally cylindrical inner electrode and the outer generally cylindrical electrode by application of an asymmetric waveform voltage and a direct current compensation voltage.

15. An apparatus according to claim 11, comprising a temperature controller for controllably establishing a temperature gradient within a gas flow between the second generally cylindrical inner electrode and the outer generally cylindrical electrode.

16. An apparatus according to claim 11, comprising a temperature controller for controllably adjusting a temperature of at least one of the second generally cylindrical inner electrode and the outer generally cylindrical electrode.

17. An apparatus according to claim 11, wherein the actuator is for translating the second generally cylindrical inner electrode synchronously with the first generally cylindrical inner electrode, such that the gap between the first generally cylindrical inner electrode and the second generally cylindrical inner electrode is approximately constant during translation.

18. A method for controllably varying specificity of a FAIMS-based ion separation, comprising:

providing a FAIMS analyzer region having an ion inlet for introducing a flow of ions thereto and having an ion outlet for extracting a subset of the flow of ions that is selectively transmitted along an average ion flow path between the ion inlet and the ion outlet;

introducing a first flow of ions into the FAIMS analyzer region via the ion inlet;

selectively transmitting a subset of the first flow of ions along the average ion flow path through the FAIMS analyzer region;

relatively translating the ion inlet relative to the ion outlet so as to provide an average ion flow path of a different length through the FAIMS analyzer region; and

,

transmitting ions of a second flow of ions along the average ion flow path of a different length.

19. A method according to claim 18, wherein an ion composition of the first flow of ions is substantially the same as an ion composition of the second flow of ions.

20. A method according to claim 19, wherein transmitting ions of the second flow of ions comprises selectively transmitting a subset of the second flow of ions, an ion composition of the subset of the second flow of ions being different than an ion composition of the subset of the first flow of ions.

21. A method according to claim 19, wherein transmitting ions of the second flow of ions comprises transmitting a set of ions having an ion composition substantially the same as the ion composition of the second flow of ions.

22. A method according to claim 18, wherein the FAIMS analyzer region is defined by a space between a first electrode surface and a second electrode surface, the first electrode surface disposed in a facing arrangement relative to the second electrode surface and spaced apart therefrom along a first direction, the first electrode surface defining the ion inlet and the second electrode surface defining the ion outlet, and wherein changing the length of the average ion flow path comprises relatively translating the first electrode surface relative to the second electrode surface along a second direction normal to the first direction.

23. An apparatus for controllably varying specificity of a FAIMS-based ion separation, comprising:

a FAIMS analyzer region defined between a first electrode surface and a second other electrode surface, the FAIMS analyzer region in communication with an ionization source for providing a flow of ions including a known ion, and with an ion outlet for extracting ions including the known ion from the FAIMS analyzer region, the first and second electrode surfaces for having an asymmetric waveform voltage applied thereacross for defining an average ion flow path for the known ion traversing therebetween; and

,

an actuator for controllably moving the first electrode surface relative to the second electrode surface for controllably varying the length of the average ion flow path.

24. An apparatus for controllably varying specificity of a FAIMS-based ion separation, comprising:

a FAIMS analyzer region controllably switchable between a low specificity mode of operation and a high specificity mode of operation and defined by at least a space between a plurality of spaced-apart electrode surfaces, the FAIMS analyzer region in communication with an ionization source for providing a flow of ions including a known ion, and with an ion outlet for extracting ions including the known ion from the FAIMS analyzer region,

wherein switching between the low specificity mode of operation and the high specificity mode of operation is accomplished by repositioning at least one electrode surface of the plurality of spaced-apart electrode surfaces relative to another electrode surface of the plurality of spaced-apart electrode surfaces.