Cytometer analysis cartridge optical configuration

• US 7,277,166 B2
• Filed: 05/16/2005
• Issued: 10/02/2007
• Est. Priority Date: 08/02/2000
• Status: Expired due to Term

• Current Assignee: Honeywell International Inc.
• Original Assignee: Honeywell International Inc.
• Inventors: Padmanabhan, Aravind, Fritz, Bernard S., Cabuz, Cleopatra, Satren, Ernest A., Schwichtenberg, Jay G.
• Primary Examiner(s): Toatley, Jr.; Gregory J.
• Assistant Examiner(s): ALLI, IYABO
• Application Number:
  US10/908,543
• Publication Number:
  US 20050243304A1
• Time in Patent Office: 869 Days
• Field of Search: 356/39, 356/244, 356/335
• US Class Current: 356/244
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0636 Focussing flows, e.g. to laminate flows
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0633 with moving parts
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: G01N 15/1456 without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 15/1484 microstructural devices
• CPC Class Codes: G01N 2015/016 White blood cells
• CPC Class Codes: G01N 2015/1452 Adjustment of focus; Alignment
• CPC Class Codes: G01N 2015/1486 Counting the particles

Abstract

A medium having microfluidic circuitry for sampling and analyses. The medium may be a cartridge having a window countersunk into it and containing a flow channel. The flow channel may have items of interest flowing through it. Analyses of these items may be optical involving one or more light sources emanating light to and one or more light detectors receiving light from the channel. There are various configurations so that source and detector light cones may reach the flow channel without obscuration or interference of the light to and from the flow channel in the window.

Claims

1. A microfluidic cartridge comprising:

a first member having a first surface and a second surface approximately parallel to each other and at a first distance from each other;

a second member having a first surface and a second surface approximately parallel to each other and at a second distance from each other; and

wherein;

the second surface of the first member is situated on the first surface of the second member;

the second member has a target area at the first surface;

the first member has an opening from the first surface through the second surface;

the opening is located over the target area; and

a cone having a vertex situated at the target area, the cone having a vertex angle with a cosine greater than 0.1.

2. The cartridge of claim 1, wherein:

the opening has an edge on one side at the first surface of the first member;

a point at a center of the edge has a distance y to the first surface of the second member along a line approximately perpendicular to the first surface of the second member; and

the point has a distance z from the edge along a straight line in the opening to the target area.

3. The cartridge of claim 2, wherein y/z is less than 0.3.

4. The cartridge of claim 3, wherein y/z is less than 0.26.

5. The cartridge of claim 3, wherein:

the cone has a vertex angle with a cosine equal to or greater than 0.5; and

the cone has a direction of about 45 degrees relative to the first surface of the second member.

6. The cartridge of claim 5, wherein if y/z is not less than 0.26, then a portion of material of the first member proximate to the point at the edge is removed to increase z.

7. The cartridge of claim 1, wherein the cone has a direction approximately 45 degrees relative to the second surface of the second member.

8. The cartridge of claim 6, wherein the target area is in a flow channel having a flow direction.

9. The cartridge of claim 8, wherein:

the opening has a width dimension and a length dimension parallel to the first surface of the first member; and

the length dimension is greater than the width dimension.

10. The cartridge of claim 9, wherein:

the cone has a portion closest to the edge; and

the edge is approximately perpendicular to the flow direction.

11. The cartridge of claim 10, wherein:

the cone has a portion closest to the edge; and

the edge is approximately parallel to the flow direction.

12. The cartridge of claim 3, wherein:

the cone has a portion closest to the edge; and

a direction of the cone is tilted approximately in a direction parallel to the flow direction.

13. The cartridge of claim 12, wherein:

y/z is less than 0.2; and

a portion of material of the first member proximate to the point at the edge is removed to increase z.

14. A cartridge comprising:

a first layer having a target area on a first surface; and

a second layer situated on the first surface of the first layer; and

wherein;

the second layer has an opening to the target area;

the opening has a location on an upper edge at a distance y from the first surface along a straight line approximately perpendicular to the first surface;

the point is at a distance z along a straight line in the opening to the target area; and

y/z<

0.3.

15. The cartridge of claim 14, wherein y/z<

0.26.

16. The cartridge of claim 14, wherein:

the target area is in a flow channel; and

a detector having a cone of detection with an apex focused on the flow channel via the open area.

17. The cartridge of claim 16, wherein:

the first layer has a second surface opposite of the first surface of the first layer;

the flow channel is optically accessible from the second surface; and

a source having a cone of radiation focused on the flow channel via the second surface.

18. The cartridge of claim 17, wherein the cartridge has a fluidic circuit connected to the flow channel.

19. A cartridge comprising:

a layer having a first surface and a second surface; and

wherein;

the first surface is approximately parallel to the second surface;

the first surface has an opening into the first layer;

a target area is situated in the opening;

the opening has a point on an upper edge of the first surface;

the point has a distance y to a plane along a line approximately perpendicular to the plane;

the plane is approximately parallel to the second surface and intersects the target area;

the point has a distance z along a straight line in the opening to the target area; and

y/z<

0.3.

20. The cartridge of claim 19, wherein y/z<

0.26.

21. The cartridge of claim 19, wherein:

the detector cone is focused on the target area through the opening;

the detector cone has a central axis intersecting the target area via the opening; and

the detector cone has a cone angle that ranges up to 65 degrees.

22. The cartridge of claim 21, wherein:

the target area is optically accessible from the first surface; and

a light source has a source cone focused on the target area via the first surface.

23. The cartridge of claim 22, wherein the source cone has a cone angle that ranges up to 50 degrees.

24. The cartridge of claim 23, wherein:

the cartridge is a removable media of a particle analyzer; and

the target area is in a flow channel of the particle analyzer.

25. The cartridge of claim 24, wherein the cartridge further comprises a fluidic mechanism.

26. The cartridge of claim 25, wherein the flow channel is for containing a core stream of particles which emit light when impinged by light from the light source.