Systems and methods for wavelength selective measurement of properties of small volume liquid samples
First Claim
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1. An apparatus comprising:
- a source of electromagnetic radiation;
a first optical system;
an optical delivery system capable of providing electromagnetic radiation from said source to said first optical system;
said first optical system being capable of substantially collimating the provided electromagnetic radiation;
a sample holding assembly capable of holding a microvolume liquid sample and placing the microvolume liquid sample in an optical path of the substantially collimated electromagnetic radiation;
the substantially collimated electromagnetic radiation also being transmitted through at least a portion of said sample holding assembly;
a filter assembly comprising at least one filter, said filter assembly being positioned in the optical path of the substantially collimated electromagnetic radiation;
a second optical system capable of receiving electromagnetic radiation transmitted through the sample and said at least a portion of said sample holding assembly and through said filter assembly;
a detecting system capable of detecting electromagnetic radiation; and
,an optical transmission system capable of receiving electromagnetic radiation from said second optical system and transmitting the received electromagnetic radiation to said detecting system;
said second optical system being also capable of transmitting electromagnetic radiation to said optical transmission system; and
said detecting system being optical disposed to receive electromagnetic radiation from said optical transmission system.
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Abstract
A system for measuring properties of small volume liquid samples, where the system includes wavelength selective filters.
28 Citations
26 Claims
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1. An apparatus comprising:
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a source of electromagnetic radiation; a first optical system; an optical delivery system capable of providing electromagnetic radiation from said source to said first optical system; said first optical system being capable of substantially collimating the provided electromagnetic radiation; a sample holding assembly capable of holding a microvolume liquid sample and placing the microvolume liquid sample in an optical path of the substantially collimated electromagnetic radiation;
the substantially collimated electromagnetic radiation also being transmitted through at least a portion of said sample holding assembly;a filter assembly comprising at least one filter, said filter assembly being positioned in the optical path of the substantially collimated electromagnetic radiation; a second optical system capable of receiving electromagnetic radiation transmitted through the sample and said at least a portion of said sample holding assembly and through said filter assembly; a detecting system capable of detecting electromagnetic radiation; and
,an optical transmission system capable of receiving electromagnetic radiation from said second optical system and transmitting the received electromagnetic radiation to said detecting system; said second optical system being also capable of transmitting electromagnetic radiation to said optical transmission system; and said detecting system being optical disposed to receive electromagnetic radiation from said optical transmission system. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for restricting, in an apparatus for measuring properties of microvolume liquid samples, transmission of electromagnetic radiation to a predetermined wavelength region, the method comprising the steps of:
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locating a pre-selected filter in an optical path of collimated electromagnetic radiation between a sample retaining assembly and an imaging optical system; the pre-selected filter being adjacent to the sample retaining assembly and to the imaging optical system; and whereby the pre-selected filter substantially restricts transmission to the pre-determined wavelength region. - View Dependent Claims (12, 13)
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14. A method for restricting, in an apparatus for measuring properties of microvolume liquid samples, transmission of electromagnetic radiation to a predetermined wavelength region, the method comprising the steps of:
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locating a pre-selected filter in an optical path of collimated electromagnetic radiation between a transmission system capable of receiving electromagnetic radiation and directing it to a detecting system, and a sample retaining assembly capable of hold no a microvolume liquid sample and for placing the microvolume liquid sample in the optical path of collimated electromagnetic radiation; the pre-selected filter being adjacent to the sample retaining assembly and receiving electromagnetic radiation transmitted through the microvolume liquid sample; and whereby the pre-selected filter substantially restricts transmission to the pre-determined wavelength region. - View Dependent Claims (15, 16)
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17. An apparatus comprising:
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a source of electromagnetic radiation; a first optical system; means for providing electromagnetic radiation from said source to said first optical system; said first optical system being capable of substantially collimating the provided electromagnetic radiation; means for holding a microvolume liquid sample and for placing the microvolume liquid sample in an optical path of the substantially collimated electromagnetic radiation;
the substantially collimated electromagnetic radiation also being transmitted through at least a portion of said holding means;a filter assembly comprising at least one filter, said filter assembly being positioned in the optical path of the substantially collimated electromagnetic radiation; a second optical system capable of receiving electromagnetic radiation transmitted through the sample and said at least a portion of said sample holding assembly and through said filter assembly; means for detecting electromagnetic radiation; and
,means for receiving electromagnetic radiation from said second optical system and transmitting the received electromagnetic radiation to said detecting means; said second optical system being also capable of transmitting electromagnetic radiation to said receiving/transmitting means; and said detecting means being optical disposed to receive electromagnetic radiation from said receiving/transmitting means. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 26)
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Specification