Measuring spectral lines from an analyte using multiplexed holograms and polarization manipulation

• US 7,277,210 B2
• Filed: 07/21/2005
• Issued: 10/02/2007
• Est. Priority Date: 07/21/2005
• Status: Expired due to Fees

• Current Assignee: Redox Biomedical, Inc.
• Original Assignee: C8 MediSensors, Inc.
• Inventors: Lipson, Jan
• Primary Examiner(s): Boutsikaris; Leonidas
• Application Number:
  US11/187,350
• Publication Number:
  US 20070019262A1
• Time in Patent Office: 803 Days
• Field of Search: 359/15, 359/494, 359/496, 385/11, 385/24, 600/317, 600/365, 356/319, 356/320
• US Class Current: 359/15
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/0224 using polarising or depolarising elements
• CPC Class Codes: G01J 3/1838 Holographic gratings
• CPC Class Codes: G01J 3/447 Polarisation spectrometry
• CPC Class Codes: G01N 21/21 Polarisation-affecting properties G01N21/19 takes precedence
• CPC Class Codes: G01N 21/65 Raman scattering
• CPC Class Codes: G02B 27/1006 for splitting or combining different wavelengths G02B27/1086, G02B27/141 take precedence
• CPC Class Codes: G02B 27/1086 operating by diffraction only
• CPC Class Codes: G02B 27/145 having sequential partially reflecting surfaces
• CPC Class Codes: G02B 27/283 used for beam splitting or combining
• CPC Class Codes: G02B 5/32 Holograms used as optical elements
• CPC Class Codes: G03H 1/26 Processes or apparatus specially adapted to produce multiple sub- holograms or to obtain images from them, e.g. multicolour technique
• CPC Class Codes: G03H 1/2645 Multiplexing processes, e.g. aperture, shift, or wavefront multiplexing
• CPC Class Codes: G03H 2001/266 Wavelength multiplexing
• CPC Class Codes: G03H 2222/31 Polarised light

Abstract

An optical apparatus in which multiplexed holograms are used to achieve wavelength selectivity and polarization manipulation is used to facilitate near-normal incidence of light on the holograms. The polarization manipulation allows light reflected from the holograms to be separated from the light incident on the holograms. In one application, the apparatus can be used to extract spectral lines of an analyte from radiation scattered from a sample.

Claims

1. An apparatus for routing selected wavelength bands in incoming light comprising:

an entry for receiving incoming light;

a polarization-sensitive assembly positioned to separate the incoming light by polarization;

one or more holographic assemblies containing multiplexed holograms for selectively reflecting the selected wavelength bands of the polarization-separated light; and

an exit separate from the entry, where the polarization-sensitive assembly is also positioned to direct the reflected light to the exit.

2. The apparatus of claim 1 in which the angles of incidence of the polarization-separated light upon the multiplexed holograms are in the range of 70-90 degrees with respect to the holographic fringes.

3. The apparatus of claim 1 in which the incoming light is from a sample and the selected wavelength bands are selected based on spectral lines of one or more analytes to be detected.

4. The apparatus of claim 1 in which the incoming light is from one or more optical emitters that produce at least two wavelengths.

5. The apparatus of claim 3 in which a full reflection bandwidth at one-half maximum of at least one of the multiplexed holograms is between 10 and 100 cm⁻

1 wavenumbers and lies in the spectral region 250-20,000 nm.

6. The apparatus of claim 3 in which an angular half-field of view at one-half maximum for at least one of the multiplexed holograms is at least 0.01 radians.

7. The apparatus of claim 1 further comprising:

one or more detectors positioned to receive the reflected light from the exit.

8. The apparatus of claim 7 in which the detector(s) consist of a single detector.

9. The apparatus of claim 7 in which the detector(s) comprise an array of detectors.

10. The apparatus of claim 9 in which at least one of the holographic assemblies contains multiplexed holograms disposed at different angles so that light from at least two different selected wavelength bands is reflected to different detectors in the array.

11. The apparatus of claim 7 in which the polarization-sensitive assembly also recombines the reflected light with respect to polarization so that, for at least one wavelength band, incoming light of different polarizations is routed to the same detector.

12. The apparatus of claim 7 in which the polarization-sensitive assembly does not recombine the reflected light with respect to polarization so that, for at least one wavelength band, incoming light of orthogonal polarizations is routed to two different detectors.

13. The apparatus of claim 1 in which, for at least one holographic assembly, incoming light of one polarization is routed to one side of the holographic assembly and incoming light of a different polarization is routed to the other side of the holographic assembly.

14. The apparatus of claim 13 in which the holographic assembly(s) consist of one holographic assembly, and the polarization-sensitive assembly routes incoming light of one polarization to one side of the holographic assembly and routes incoming light of an orthogonal polarization to the other side of the holographic assembly.

15. The apparatus of claim 1 in which the holographic assembly(s) consist of two holographic assemblies, and the polarization-sensitive assembly routes incoming light of one polarization to one holographic assembly and routes incoming light of an orthogonal polarization to the other holographic assembly.

16. The apparatus of claim 1 in which the polarization-sensitive assembly comprises:

a polarization beam splitter to separate the incoming light into a first polarized beam and a second polarized beam having orthogonal linear polarizations; and

for each of the polarized beams;

a phase retardation plate that receives the polarized beam and converts said linear polarization of the received polarized beam into a circular polarization and directs the circularly polarized beam to one of the holographic assembly(s), and that also receives the circularly polarized beam reflected back from the holographic assembly and converts the circularly polarized beam to a linear polarization that is substantially orthogonal to said linear polarization of the received polarized beam and directs the linearly polarized beam back to the polarization beam splitter.

17. The apparatus of claim 16 in which the phase retardation plate is a quarter waveplate.

18. The apparatus of claim 1 further comprising:

an entry for a second stage for receiving light transmitted by at least one of the holographic assembly(s);

one or more holographic assemblies for the second stage containing second multiplexed holograms for selectively reflecting second, different selected wavelength bands of the received light;

an exit for the second stage that is separate from the entry for the second stage; and

a polarization-sensitive assembly for the second stage positioned to route the received light from the entry of the second stage to the holographic assembly(s) of the second stage, to the exit of the second stage.

19. An apparatus for routing selected wavelength bands in incoming light comprising:

a polarization-sensitive assembly positioned to separate incoming light by polarization;

one or more holographic assemblies containing multiplexed holograms for selectively reflecting the selected wavelength bands of the polarization-separated light; and

where the polarization-sensitive assembly is also positioned to direct the reflected light in two or more different directions so that incoming light of different polarizations is routed to different directions.

20. The apparatus of claim 19 in which the angles of incidence of the polarization-separated light upon the multiplexed holograms are in the range of 70-90 degrees with respect to the holographic fringes.

21. The apparatus of claim 19 further comprising:

two or more detectors positioned to receive the reflected light routed to different directions.

22. The apparatus of claim 21 in which the incoming light is from a sample and the selected wavelength bands are selected based on spectral lines of one or more analytes to be detected.

23. The apparatus of claim 22 further comprising:

logic for combining detector signals from said different detectors to which incoming light of different polarizations is routed.

24. The apparatus of claim 22 further comprising:

logic for combining detector signals from a first detector and a second detector, where the polarization-sensitive assembly separates the incoming light into two orthogonal linear polarizations and routes light of one linear polarization to the first detector and routes light of the other linear polarization to the second detector.

25. The apparatus of claim 24 in which combining the detector signals reduces noise.

26. The apparatus of claim 24 in which the logic calculates a difference based on the two detector signals.

27. The apparatus of claim 24 in which the logic scales the detector signals and calculates a difference of the scaled detector signals.

28. The apparatus of claim 24 in which the incoming light is from a sample;

the selected wavelength bands are selected based on spectral lines of one or more analytes to be detected;

the incoming light from the analytes is predominantly polarized along a first linear polarization that is directed to the first detector;

noise is predominantly unpolarized; and

the logic scales the detector signals and calculates a difference of the scaled detector signals.

29. The apparatus of claim 24 in which the holographic assembly(s) consist of one holographic assembly, and the polarization-sensitive assembly routes incoming light of one linear polarization to one side of the holographic assembly and routes incoming light of the other linear polarization to the other side of the holographic assembly.

30. The apparatus of claim 24 in which the polarization-sensitive assembly comprises:

a polarization beam splitter to separate the incoming light into the two orthogonal linear polarizations; and

for each of the two linear polarized beams;

a phase retardation plate that receives the polarized beam and converts said linear polarization of the received polarized beam into a circular polarization and directs the circularly polarized beam to one of the holographic assembly(s), and that also receives the circularly polarized beam reflected back from the holographic assembly and converts the circularly polarized beam to a linear polarization that is substantially orthogonal to said linear polarization of the received polarized beam and directs the linearly polarized beam back to the polarization beam splitter.

31. The apparatus of claim 19 in which the incoming light is from one or more optical emitters that produce two distinct polarizations.

32. An apparatus for routing selected wavelength bands in incoming light comprising:

a first stage comprising;

a first entry for receiving incoming light;

a first polarization-sensitive assembly positioned to separate the incoming light by polarization;

one or more first holographic assemblies containing first multiplexed holograms for selectively reflecting first selected wavelength bands of the polarization-separated light;

a first exit, where the first polarization-sensitive assembly is also positioned to direct light reflected from the first holographic assembly(s) to the first exit; and

a second stage comprising;

a second entry for receiving light transmitted by at least one of the first holographic assembly(s);

one or more second holographic assemblies containing second multiplexed holograms for selectively reflecting second selected wavelength bands of the received light, where the second selected wavelength bands are different from the first selected wavelength bands;

a second exit; and

an active polarization rotator that can be switched between activating and not activating the second stage, where activating the second stage results in routing the received light to the second holographic assembly(s) and then to the second exit.

33. The apparatus of claim 32 further comprising:

one or more detectors positioned to receive the reflected light from the first exit, where light routed by the active polarization rotator to the second exit will propagate to the same detector(s) via the first exit.

34. The apparatus of claim 32 further comprising:

one or more first detectors positioned to receive the reflected light from the first exit; and

one or more second detectors separate from the first detector(s), positioned to receive light routed by the active polarization rotator to the second exit.

35. The apparatus of claim 32 further comprising:

one or more detectors positioned to receive light from the first exit and/or the second exit when the second stage is activated and when the second stage is not activated.

36. The apparatus of claim 35 in which the detector(s) are spatially multiplexed to receive light when the second stage is activated and when the second stage is not activated.

37. The apparatus of claim 35 in which the detector(s) are time multiplexed to receive light when the second stage is activated and when the second stage is not activated.

38. The apparatus of claim 35 further comprising:

logic for combining a detector signal from the detector(s) when the second stage is activated and a detector signal from the detector(s) when the second stage is not activated.

39. The apparatus of claim 38 in which combining the detector signals reduces noise.

40. The apparatus of claim 38 in which the logic calculates a difference based on the detector signals.

41. The apparatus of claim 38 in which the logic scales the detector signals and calculates a difference of the scaled detector signals.

42. The apparatus of claim 38 in which the detector signal for the not activated second stage is based on spectral lines of one or more analytes to be detected;

and the detector signal for the activated second stage is based on spectral lines of the analyte(s) to be detected and spectral lines of one or more other substances.

43. The apparatus of claim 38 in which the detector signal for the not activated second stage is based on spectral lines of one or more analytes to be detected;

and the detector signal for the activated second stage is based on spectral lines of the analyte(s) to be detected and spectral lines of a solvent in which the analyte(s) are dissolved.

44. The apparatus of claim 32 in which the first polarization-sensitive assembly separates the incoming light into two orthogonal linear polarizations and the active polarization rotator routes only one of the polarizations to the second exit.

45. The apparatus of claim 32 in which the active polarization rotator is based on polarization rotation in liquid crystals.

46. The apparatus of claim 32 further comprising:

a third stage comprising;

a third entry for receiving light from the second stage when the second stage is not activated;

one or more third holographic assemblies containing third multiplexed holograms for selectively reflecting third selected wavelength bands of the received light, where the third selected wavelength bands are different from the first and second selected wavelength bands;

a third exit; and

an active polarization rotator that can be switched between activating and not activating the third stage, where activating the third stage results in routing the received light to the third holographic assembly(s) and then to the third exit.

47. The apparatus of claim 32 in which the incoming light is from one or more optical emitters that produce at least two wavelengths.

48. The apparatus of claim 32 in which the incoming light is from one or more optical emitters that produce distinct polarizations.

49. The apparatus of claim 32 in which the second stage further comprises:

a non-reciprocal polarization rotator that rotates polarization of light propagating in one direction by 90 degrees differently than light propagating in an opposite direction.