Trace gas sensor with reduced degradation

• US 7,278,291 B2
• Filed: 02/06/2006
• Issued: 10/09/2007
• Est. Priority Date: 02/07/2005
• Status: Active Grant

• Current Assignee: Circassia AB
• Original Assignee: Apieron Biosystems Corporation
• Inventors: Chazan, David J., Anvar, David J., Talbott, Autumn
• Primary Examiner(s): LARKIN, DANIEL SEAN
• Application Number:
  US11/348,925
• Publication Number:
  US 20060191321A1
• Time in Patent Office: 610 Days
• Field of Search: 73/232, 73/310.5
• US Class Current: 73/31.05
• CPC Class Codes: G01N 33/497 of gaseous biological material, e.g. breath

Abstract

The degradation over time that is commonly seen with analyte-binding proteins when used as sensors for trace amounts of an analyte in a gaseous mixture is reduced by maintaining the sensor in a low-oxygen or oxygen-free environment.

Claims

1. A method for preserving a nitric oxide sensing element in an exhaled breath analyzer against loss of sensitivity during storage, said sensing element comprising a nitric oxide binding protein and said method comprising maintaining said sensing element in a low-oxygen environment or an environment that is substantially free of oxygen.

2. The method of claim 1 wherein said nitric oxide binding protein is cytochrome c.

3. The method of claim 1 wherein said sensing element comprises said nitric oxide binding protein encapsulated in a xerogel.

4. The method of claim 1 wherein said sensing element comprises cytochrome c encapsulated in a xerogel.

5. The method of claim 1 further comprising maintaining said sensing element in an environment in which the relative humidity is 6% or less.

6. The method of claim 1 further comprising maintaining said sensing element in an environment in which the relative humidity is 3% to 6%.

7. The method of claim 1 further comprising maintaining said sensing element in an environment in which the relative humidity is 1% or less.

8. The method of claim 7 wherein said relative humidity is 0.5% or less.

9. The method of claim 7 wherein said relative humidity is 0.1% or less.

10. The method of claim 1 comprising maintaining said sensing element in an environment containing 0.1% oxygen by volume or less.

11. The method of claim 1 comprising maintaining said sensing element in an environment containing 0.1 ppm oxygen by volume or less.

12. The method of claim 1 wherein said sensing element has a surface area that is greater than 300 m²/g.

13. The method of claim 1 wherein said sensing element has a surface area that is greater than 390 m²/g.

14. The method of claim 1 wherein said sensing element comprises cytochrome c encapsulated in a xerogel, has a surface area that is greater than 300 m²/g, and is maintained in an environment containing 0.1% oxygen by volume or less and a relative humidity of 0.1% or less.

15. A device for sensing trace amounts of nitric oxide in exhaled breath, said device comprising a sensing element comprising a nitric oxide binding protein suspended in a support matrix and packaged in an environment that is low in oxygen or substantially free of oxygen.

16. The device of claim 15 wherein said nitric oxide binding protein is cytochrome c.

17. The device of claim 15 wherein said sensing element comprises said nitric oxide binding protein encapsulated in a xerogel.

18. The device of claim 15 wherein said sensing element comprises cytochrome c encapsulated in a xerogel.

19. The device of claim 15 wherein said environment has a relative humidity of 6% or less.

20. The device of claim 19 wherein said relative humidity is 0.5% or less.

21. The device of claim 19 wherein said relative humidity is 0.1% or less.

22. The device of claim 15 wherein said environment has a relative humidity is 3% to 6%.

23. The device of claim 15 wherein said environment has a relative humidity of 1% or less.

24. The device of claim 15 wherein said environment has an oxygen content of 0.1% by volume or less.

25. The device of claim 15 wherein said environment has an oxygen content of 0.1 ppm by volume or less.

26. The device of claim 15 wherein said sensing element has a surface area greater than 300 m²/g.

27. The device of claim 15 wherein said sensing element has a surface area greater than 390 m²/g.

28. The device of claim 15 wherein said nitric oxide binding protein is cytochrome c, said support matrix is a xerogel with a surface area greater than 300 m²/g, and said environment has an oxygen content of 0.1% by volume or less, and a relative humidity of 0.1% or less.