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Methods for diagnosis of low grade astrocytoma

  • US 7,279,292 B2
  • Filed: 08/17/2006
  • Issued: 10/09/2007
  • Est. Priority Date: 10/11/2001
  • Status: Expired due to Fees
First Claim
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1. A method for identifying low grade astrocytoma cells in a biological sample, comprising the steps of:

  • preparing slides from a biological sample;

    serially incubating said biological sample in at least ethanol and hydrogen peroxide;

    heating said slides in an unmasking solution including a mixture of at least sodium citrate and citric acid;

    incubating the biological sample with anti-J1-31 antibody under conditions in which the antibody binds to J1-31 antigen in the sample, said anti-J1-31 antibody being produced by the hybridoma cell line J1-31, ATCC designation number CRL-2253;

    contacting the biological sample with a secondary antibody selected so as to form an immune complex with the anti-J1-31 antibody bound to J1-31 antigen;

    detecting cells expressing the J1-31 antigen by detecting the immune complex; and

    detecting astrocytes in the sample in which the immune complex is not present, to thereby identify low-grade astrocytoma cells.

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