Reactive probes for two-photon fluorescent imaging and sensing
First Claim
1. A method of multiphoton imaging comprising exciting a two-photon reactive probe structure of the formula:
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Abstract
Fluorescent dyes and probes are key components in multiphoton based fluorescence microscopy imaging of biological samples. In order to address the demand for better performing dyes for two-photon based imaging, a new series of reactive fluorophores tailored for multiphoton imaging has been disclosed. These fluorophores are based upon the fluorene ring system, known to exhibit high fluorescence quantum yields, typically >0.7, and possess high photostability. They have been functionalized with moieties to act, e.g., as efficient amine-reactive fluorescent probes for the covalent attachment onto, e.g., proteins and antibodies. The synthesis and the single-photon spectral characteristics, as well as measured two-photon absorption cross sections of the reactive fluorophores in solution are presented. Spectral characterizations of bovine serum albumin (BSA) conjugated with the new reactive probe is presented.
31 Citations
3 Claims
- 1. A method of multiphoton imaging comprising exciting a two-photon reactive probe structure of the formula:
Specification
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- Resources
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Current AssigneeUniversity of Central Florida (State University System of Florida)
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Original AssigneeUniversity of Central Florida (State University System of Florida)
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InventorsBelfield, Kevin D., Schafer, Katherine J.
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Primary Examiner(s)McKane; Joseph K.
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Assistant Examiner(s)Barker; Michael P.
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Application NumberUS11/786,290Time in Patent Office188 DaysField of Search514/367, 514/423, 514/365, 514/2, 514/75, 514/80, 514/92, 514/336, 514/375US Class Current514/367CPC Class CodesC07D 277/66 with aromatic rings or ring...
