System and method for self-referencing a sensor in a micron-sized deep flow chamber
First Claim
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1. A method for self-referencing a sensor associated with a micron-sized deep flow channel, said method comprising the steps of:
- directing an optical beam at said sensor which has a single sensing region divided into a detection region and a reference region which are contiguous to one another and which are respectively interfaced with a sample solution and a reference solution that flow side-by-side to one another in a longitudinal direction within said micron-sized deep flow channel;
receiving an output optical beam from said sensor;
analyzing the output optical beam to determine a detection signal associated with the sample solution flowing in the detection region of said sensor and to determine a reference signal associated with the reference solution flowing in the reference region of said sensor; and
subtracting the reference signal from the detection signal to generate a corrected detection signal, wherein uncertainties in the detection signal due to environmental conditions and noise generated by components in an interrogation system are reduced in the corrected detection signal.
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Abstract
A system and method are described herein for self-referencing a sensor that is used to detect a biomolecular binding event and/or kinetics which occur in a sample solution flowing along side a reference solution in a micron-sized deep flow channel.
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Citations
17 Claims
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1. A method for self-referencing a sensor associated with a micron-sized deep flow channel, said method comprising the steps of:
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directing an optical beam at said sensor which has a single sensing region divided into a detection region and a reference region which are contiguous to one another and which are respectively interfaced with a sample solution and a reference solution that flow side-by-side to one another in a longitudinal direction within said micron-sized deep flow channel; receiving an output optical beam from said sensor; analyzing the output optical beam to determine a detection signal associated with the sample solution flowing in the detection region of said sensor and to determine a reference signal associated with the reference solution flowing in the reference region of said sensor; and subtracting the reference signal from the detection signal to generate a corrected detection signal, wherein uncertainties in the detection signal due to environmental conditions and noise generated by components in an interrogation system are reduced in the corrected detection signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. An interrogation system capable of performing the following steps:
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directing an optical beam at a sensor which has a single sensing region divided into a detection region and a reference region which are contiguous to one another and which are respectively interfaced with a sample solution and a reference solution that flow side-by-side to one another in a longitudinal direction within a micron-sized deep flow channel; receiving an output optical beam from said sensor; wherein the output optical beam is analyzed to determine a detection signal associated with the sample solution flowing in the detection region of said sensor and to determine a reference signal associated with the reference solution flowing in the reference region of said sensor; and wherein the reference signal is subtracted from the detection signal to generate a corrected detection signal; and wherein the corrected detection signal indicates whether or not a biomolecular binding event occurred in the sample solution flowing in said micron-sized deep flow channel. - View Dependent Claims (14, 15, 16, 17)
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Specification