FAIMS apparatus and method for separating ions in the gas phase

• US 7,285,774 B2
• Filed: 09/23/2003
• Issued: 10/23/2007
• Est. Priority Date: 09/25/2002
• Status: Expired due to Fees

• Current Assignee: Thermo Finnigan LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Thermo Finnigan LLC (Thermo Fisher Scientific Incorporated)
• Inventors: Guevremont, Roger
• Primary Examiner(s): Wells; Nikita
• Assistant Examiner(s): Johnston; Phillip A.
• Application Number:
  US10/529,304
• Publication Number:
  US 20060038121A1
• Time in Patent Office: 1,491 Days
• Field of Search: 250/290
• US Class Current: 250/290
• CPC Class Codes: G01N 27/624 Differential mobility spectrometry [DMS]; Field asymmetric-waveform ion mobility spectrometry [FAIMS]
• CPC Class Codes: H01J 49/063 Multipole ion guides, e.g. quadrupoles, hexapoles

Abstract

Disclosed is a high field asymmetric waveform ion mobility spectrometer (FAIMS) including a set of spaced-apart parallel rods, the space between the parallel rods having first and second ends and defining an analyzer region. The apparatus includes an electrical controller for electrically coupling to the set of parallel rods, for applying at least an rf-voltage between the parallel rods of the set of parallel rods in a first operating mode and for applying a combination of an asymmetric waveform voltage and a direct current voltage between the parallel rods of the set of parallel rods in a second operating mode.

Claims

1. A method of separating ions comprising the steps of:

providing an analyzer region that is operable in both an rf-only mode and in a FAIMS mode;

introducing ions into the analyzer region;

effecting a selective separation of the ions within the analyzer region substantially during operation in the FAIMS mode;

controllably switching the analyzer region from the FAIMS mode to the rf-only mode; and

,

extracting the selectively separated ions from the analyzer region substantially during operation in the rf-only mode.

2. A method according to claim 1, comprising a step prior to the step of effecting a separation of the ions of:

trapping some of the introduced ions within the analyzer region by the application of selected electric potentials at the ends of the analyzer region.

3. A method according to claim 1, wherein the analyzer region is provided as a space between a set of parallel rods, the space having first and second ends.

4. A method according to claim 1, wherein the ions are introduced into the analyzer region substantially during operation of the analyzer region in the rf-only mode.

5. A method according to claim 4, comprising a step prior to the step of selectively separating ions of:

controllably switching the analyzer region from the rf-only mode to the FAIMS mode.

6. A method according to claim 5, comprising a step prior to the step of controllably switching the analyzer region from the rf-only mode to the FAIMS mode of:

collisionally cooling the ions so as to confine the ions within a volume that is smaller than a volume occupied by the ions prior to collisional cooling.

7. A method according to claim 1, wherein the ions are introduced into the analyzer region substantially during operation of the analyzer region in the FAIMS mode.

8. A method according to claim 6, comprising a step prior to the step of extracting ions of:

collisionally cooling the selectively separated ions.

9. A method according to claim 8, comprising a step prior to the step of extracting the selectively separated ions of:

controllably switching the analyzer region from the rf-only mode to the FAIMS mode, so as to effect a selective second separation of the collisionally cooled selectively separated ions.

10. A method according to claim 2, wherein the step of extracting the selectively separated ions includes a step of applying a different selected electric potential at the second end of the analyzer region.

11. A method according to claim 1, comprising a step of providing the extracted selectively separated ions to one of a detector, an analyzer and an ion collector.

12. A method according to claim 6, comprising a step after the step of extracting the selectively separated ions of:

refilling the analyzer region with ions while the analyzer region is operating in the rf-only mode.

13. A method according to claim 3, wherein the set of parallel rods has a quadrupole configuration.

14. A method according to claim 13, wherein each parallel rod of the set of parallel rods includes a plurality of coaxially aligned segments.

15. A method according to claim 14, wherein the selectively separated ions are extracted from the analyzer region as a result of an electric field established within the analyzer region by application of different dc voltages between different sets of segments of die parallel rods.

16. A method according to claim 1, wherein the gas pressure in the analyzer region is in the range between 10² torr to 10⁻

6 torr.

17. A method according to claim 1, wherein the gas pressure in the analyzer region is in the range between 10 torr to 10⁻

4 torr.

18. A method according to claim 1, wherein the gas pressure in the analyzer region is in the range between 5 torr to 10⁻

2 torr.

28. A method according to claim 3, wherein the set of parallel rods has a hexapole configuration.

29. A method according to claim 3, wherein the set of parallel rods has an octapole configuration.

30. A method according to claim 13, wherein the step of controllably switching the analyzer region from the FAIMS mode to the rf-only mode comprises controllably changing a waveform applied to pairs of opposite rods of the parallel rods, by changing a relative phase shift of two component sinusoidal waves of the waveform.

31. A method according to claim 30, wherein the step of controllably switching the analyzer region from the FAIMS mode to the rf-only mode comprises changing the dc voltages applied to the pairs of opposite rods of the quadrupole.

32. A method according to claim 13, wherein the selective separation of the ions within the analyzer region during operation in the FAIMS mode comprises a mass analysis separation.

33. A method according to claim 14, comprising establishing a potential gradient along a length of the analyzer region for trapping at least some of the introduced ions within the analyzer region.

35. A method according to claim 1, comprising a step prior to the step of extracting ions of:

collisionally cooling the selectively separated ions during operation in the rf-only mode.

36. A method according to claim 35, comprising a step prior to the step of extracting the selectively separated ions of:

controllably switching the analyzer region from the rf-only mode to the FAIMS mode, so as to effect a selective second separation of the collisionally cooled selectively separated ions.

19. An apparatus for separating ions comprising:

a set of parallel rods having a space therebetween, the space having first and second ends and defining an analyzer region; and

,

an electrical controller for electrically coupling to the set of parallel rods, for applying a radio frequency (rf)-voltage between the parallel rods of the set of parallel rods in a rf-only operating mode, for applying a combination of an asymmetric waveform voltage and a direct current voltage between the parallel rods of the set of parallel rods in a FAIMS operating mode, and for controllably switching between the rf-only operating mode and the FAIMS operating mode, wherein, during use, an ion which is being transmitted through the analyzer region is subjected to the rf-only operating mode and to the FAIMS operating mode during a period of time the ion is resident within the analyzer region.

20. An apparatus according to claim 19, comprising trapping members disposed proximate the first and second ends of the space for providing a stopping voltage, the stopping voltage for cooperating with the rf-voltage in the rf-only operating mode and for cooperating with the combination of an asymmetric waveform voltage and a direct current voltage in the FAIMS operating mode to constrain ions within the space between the first and second ends.

21. An apparatus according to claim 19, wherein the set of parallel rods has a quadrupole configuration.

22. An apparatus according to claim 19, wherein each parallel rod of the set of parallel rod comprises a plurality of coaxially aligned segments in an end-to-end arrangement.

23. An apparatus according to claim 22, comprising an electrically insulating member disposed between adjacent segments of the coaxially aligned segments within a same parallel rod.

24. An apparatus according to claim 20, wherein the trapping members comprise an ion entrance lens disposed adjacent the first end of the space and an ion exit lens disposed adjacent the second end of the space.

25. An apparatus according to claim 19, wherein the set of parallel rods includes six parallel rods.

26. An apparatus according to claim 19, wherein the set of parallel rods includes eight parallel rods.

27. An apparatus according to claim 19, comprising a housing for containing the set of parallel rods and for maintaining a predetermined atmosphere including a bath gas within the analyzer region.

34. An apparatus according to claim 21, wherein, during use, an output waveform of the electrical controller is controllably changed from the rf-voltage to the asymmetric waveform voltage by changing a relative phase shift of two component sinusoidal waves of the output waveform.