Optical device for surface-generated fluorescence
First Claim
1. A sample substrate adapted for use with electromagnetic excitation light directed to a fluorescent sample material disposed on the substrate, causing light to be emitted from the sample, comprising a base and a layer system, the layer system comprising a dielectric multilayer interference coating with at least two layers wherein thicknesses of the layers cause separation of the excitation light from the emitted light;
- wherein the thicknesses of the layers are chosen in such a way that for s-polarized light impinging on the layer system and having a wavelength corresponding to a wavelength of the emitted light and a medium of incidence of the light having an index of refraction of 1.4, less than 1.5% of light is transmitted for angles of incidence from −
70 degrees to +70 degrees.
5 Assignments
0 Petitions
Accused Products
Abstract
A sample substrate adapted for use with electromagnetic excitation light includes a base and a layer system. The layer system includes a multilayer interference coating with at least two layers wherein the thicknesses of the layers ensure that light emitted by a fluorescent sample material disposed on top of said multilayer interference coating is reflected. Light directed to a fluorescent sample material disposed on the substrate causes light to be emitted from the sample. The layer system includes a multilayer interference coating with at least two layers wherein thicknesses of the layers cause separation of the excitation light from the emitted light.
40 Citations
31 Claims
-
1. A sample substrate adapted for use with electromagnetic excitation light directed to a fluorescent sample material disposed on the substrate, causing light to be emitted from the sample, comprising a base and a layer system, the layer system comprising a dielectric multilayer interference coating with at least two layers wherein thicknesses of the layers cause separation of the excitation light from the emitted light;
wherein the thicknesses of the layers are chosen in such a way that for s-polarized light impinging on the layer system and having a wavelength corresponding to a wavelength of the emitted light and a medium of incidence of the light having an index of refraction of 1.4, less than 1.5% of light is transmitted for angles of incidence from −
70 degrees to +70 degrees.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
-
29. A sample substrate adapted for use with electromagnetic excitation light directed to a fluorescent sample material disposed on the substrate, causing light to be emitted from the sample, comprising a base and a layer system, the layer system comprising a dielectric multilayer interference coating with at least two layers wherein thicknesses of the layers cause separation of the excitation light from the emitted light;
-
wherein the emitted light is adapted to be received by a photodetector on a front side of the fluorescent sample material opposite the substrate and the thicknesses of the layers cause reflection of substantially all of the emitted light; wherein the excitation light is received at a front side of the fluorescent sample material opposite the substrate and the thicknesses of the layers cause transmission of substantially all of the excitation light which is not absorbed by the fluorescent sample material; and wherein greater than 90% of the excitation light which is not absorbed by the fluorescent sample material is transmitted at a 0 degree angle of incidence.
-
-
30. A method of manufacturing a sample substrate adapted to obtain electromagnetic emission from a sample disposed on the substrate when the sample is excited by a light comprising the steps of:
-
selecting at least two dielectric layers of a reflecting dielectric multilayer interference coating; determining thicknesses of the layers to cause separation of the excitation light from the emitted light wherein the thicknesses of the layers are chosen in such a way that for s-polarized light impinging on the layer system and having a wavelength corresponding to a wavelength of the emitted light and a medium of incidence of the light having an index of refraction of 1.4, less than 1.5% of light is transmitted for angles of incidence from −
70 degrees to +70 degrees;and depositing the layers on a base. - View Dependent Claims (31)
-
Specification