Electrochemical biosensor
First Claim
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1. An electrochemical biosensor comprising:
- a lower substrate;
an upper substrate;
a working electrode and a reference (or counter) electrode respectively formed on either the lower substrate or the upper substrate;
a reaction layer containing a glucose oxidase enzyme, electron transfer mediator, water soluble polymers, water soluble fatty acid or its salt, and quaternary ammonium salt, wherein the reaction layer is formed over the working electrode and is not formed over the reference electrode;
a spacer formed between the lower and the upper substrates, wherein the spacer is provided with a cut out pattern of a sample introducing bay, an air discharge channel, and a void at the cross of a sample introducing bay and an air discharge channel; and
wherein the electrochemical biosensor is capable of analyzing a whole blood sample.
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Abstract
There is provided the reagent layer composition that can substantially reduce the measurement bias arising from hematocrits. The addition of fatty acid (4-20 carbons) and quaternary ammonium salt to a commonly used reagent layer composition composed of an enzyme, an electron transfer mediator, and several water soluble polymers not only reduce the hematocrit level-dependent bias but also provide very stable performance for an extended period of time. Disclosed are also various types of sub microliter sample volume electrochemical biosensors that are suitable to use with the reagent layer composition of present invention.
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16 Claims
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1. An electrochemical biosensor comprising:
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a lower substrate; an upper substrate; a working electrode and a reference (or counter) electrode respectively formed on either the lower substrate or the upper substrate; a reaction layer containing a glucose oxidase enzyme, electron transfer mediator, water soluble polymers, water soluble fatty acid or its salt, and quaternary ammonium salt, wherein the reaction layer is formed over the working electrode and is not formed over the reference electrode; a spacer formed between the lower and the upper substrates, wherein the spacer is provided with a cut out pattern of a sample introducing bay, an air discharge channel, and a void at the cross of a sample introducing bay and an air discharge channel; and wherein the electrochemical biosensor is capable of analyzing a whole blood sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification