Polyepitopic protein fragments of the E6 and E7 proteins of HPV, their production and their use particularly in vaccination

• US 7,288,258 B2
• Filed: 06/02/2004
• Issued: 10/30/2007
• Est. Priority Date: 06/03/1999
• Status: Expired due to Fees

• Current Assignee: L'Assistance Publique - Hopitaux De Paris
• Original Assignee: Institut National De La Sante Et De La Recherche Medicale (Inserm) (Allianz Global Investors GmbH), Peptide Immune Ligands
• Inventors: Guillet, Jean-Gerard, Bourgault Villada, Isabelle, Ferries, Estelle, Choppin, Jeannine, Connan, Francine
• Primary Examiner(s): SALIMI, ALI REZA
• Application Number:
  US10/858,384
• Publication Number:
  US 20050033025A1
• Time in Patent Office: 1,245 Days
• Field of Search: 424/204.1, 424/186.1, 530/300, 536/23.72
• US Class Current: 424/204.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61P 31/18 for HIV
• CPC Class Codes: A61P 37/02 Immunomodulators
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 2710/20022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2740/16322 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

Polyepitopic peptides of E6 and E7 proteins of Human Papillomavirus, their production, and methods of treating pathologies in which a polyepitopic peptide of the E6 and E7 protein of Human Papillomavirus is recognized by the cellular immune system.

Claims

1. A polyepitope of E7 protein from HPV, consisting of 23 amino acids delimited by the amino acids located in positions 3 and 25 of the peptide sequence of the E7 protein of HPV, wherein said polyepitope comprises peptide sequence SEQ ID NO:

14 as follows;

(3)GDTPTLHEYMLDLQPETTDLYCY(25)

and wherein said peptide sequence comprises 5 epitopes binding stably to at least one of 6 HLA molecules of the following types;

A1, A2, B18, B35, B44 or B62, said epitopes being selected from the group consisting of;

(3)GDTPTLHEY(11) (residues 3 to 11 of SEQ ID NO;

     12) binding stably to HLA molecules of the B44 type,

(5)TPTLHEYML(13) (residues 5 to 13 of SEQ ID NO;

     12) binding stably to HLA molecules of the B35 type,

(11)YMLDLQPETT(20) (residues 11 to 20 of SEQ ID NO;

     12) binding stably to HLA molecules of the A2 type,

(15)LQPETTDL Y(23) (residues 15 to 23 of SEQ ID NO;

     12) binding stably to HLA molecules of the B62 type, and

(16)QPETTDLYCY(25) (residues 16 to 25 of SEQ ID NO;

     12) binding stably to HLA molecules of the Al or B18 type.

2. A peptide sequence consisting of the amino acids of SEQ ID NO:

14, wherein said peptide sequence binds stably to at least one of 6 HLA molecules selected from the group consisting of A1, A2, B18, B35, B44 and B62.