Compositions and methods for capturing, isolating, detecting, analyzing and quantifying macromolecules

• US 7,288,415 B2
• Filed: 05/21/2003
• Issued: 10/30/2007
• Est. Priority Date: 02/18/2000
• Status: Expired due to Fees

• Current Assignee: Aspira Biosystems, Inc. (Nanomune Technologies, Inc.)
• Original Assignee: Aspira Biosystems, Inc. (Nanomune Technologies, Inc.)
• Inventors: Huang, Chin-Shiou
• Primary Examiner(s): Chin; Christopher L.
• Application Number:
  US10/443,613
• Publication Number:
  US 20040018642A1
• Time in Patent Office: 1,623 Days
• Field of Search: None
• US Class Current: 436/518
• CPC Class Codes: B01J 20/268 Polymers created by use of a template, e.g. molecularly imprinted polymers
• CPC Class Codes: G01N 2600/00 Assays involving molecular imprinted polymers/polymers created around a molecular template
• CPC Class Codes: G01N 33/544 the carrier being organic
• CPC Class Codes: Y10S 436/804 Radioisotope, e.g. radioimmunoassay
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays
• CPC Class Codes: Y10S 977/927 Diagnostic contrast agent

Abstract

The present invention provides imprint compositions useful for capturing, isolating, detecting and/or quantifying macromolecules in a sample, methods of making and using the same. Generally, the imprint compositions comprise a matrix material defining an imprint of a template molecule, and the template molecule typically corresponds to a portion of a macromolecule of interest.

Claims

1. A method of capturing a macromolecule, comprising:

contacting the macromolecule with an imprint composition comprising a matrix, said matrix defining an imprint cavity of a template molecule that corresponds to a portion of the macromolecule, under conditions in which the macromolecule binds the imprint composition.

2. The method of claim 1, further comprising quantifying the amount of the macromolecule bound to the imprint composition.

3. The method of claim 1, further comprising removing the template molecule from the matrix.

4. A method of isolating a macromolecule, comprising:

contacting the macromolecule with an imprint composition comprising a matrix, said matrix defining an imprint cavity of a template molecule that corresponds to a portion of the macromolecule under conditions in which the macromolecule binds the imprint composition; and

recovering the macromolecule from the imprint composition.

5. The method of claim 4, further comprising quantifying the amount of the macromolecule bound to the imprint composition.

6. A method of capturing a plurality of macromolecules, comprising:

contacting the plurality of macromolecules with a spatially identifiable array of imprint cavities, wherein each imprint cavity is of a template molecule that corresponds to a portion of one of the plurality of the macromolecules, under conditions in which the macromolecules bind the imprint cavities.

7. The method of claim 6, further comprising recovering the plurality of the macromolecules from the imprint cavities.

8. The method of claim 6, further comprising removing the template molecule from the imprint cavities.

9. The method of claim 6, further comprising measuring a property of the array to quantify the plurality of macromolecules captured.

10. The method of claim 6, wherein the array includes at least two different sites defined by respective first and second imprint cavities of respectively first and second template molecules that correspond to portions respectively of two different ones of the plurality of macromolecules.

11. The method of claim 6, wherein the template molecules are peptides of three to seven amino acids in length.

12. The method of claim 6, further comprising detecting the capturing of the macromolecules bound to the array by detecting radioactive emissions from the array, wherein the macromolecules are radioactively labeled.

13. The method of claim 6, further comprising detecting the capturing of the macromolecules bound to the array by detecting an optical property of the array.

14. The method of claim 6, further comprising individually quantifying multiple different ones of the plurality of different macromolecules captured respectively at different sites on the array.

15. A method of quantifying the relative amounts of a plurality of different macromolecules in a sample, comprising:

contacting the sample with a spatially identifiable array of imprint cavities, wherein each imprint cavity is of a template molecule that corresponds to a portion of one of the plurality of the macromolecules, under conditions in which the macromolecules bind the imprint cavities;

quantifying the amount of the sample bound to at least a subset of the array of imprint cavities; and

comparing quantified amounts associated with imprint cavities corresponding with ones of the plurality of different macromolecules to determine the relative amounts.

16. The method of claim 15, further comprising recovering the plurality of the macromolecules from the imprint cavities.

17. The method of claim 15, further comprising removing the template molecule from the imprint cavities.

18. The method of claim 15, wherein quantifying the amount of the sample bound includes measuring a property of the array.

19. The method of claim 15, wherein quantifying the amount of the sample bound includes counting radioactive emissions from macromolecules that are radioactively labeled and bound to the array.

20. The method of claim 15, wherein quantifying the amount of the sample bound includes measuring an optical property of the array.