Cleavable oligonucleotide arrays
First Claim
Patent Images
1. An oligonucleotide array comprising a substrate having a surface and a plurality of features on the substrate, each feature of the plurality of features comprising A) a first population of oligonucleotides bound via a cleavable linker moiety to the surface, and B) a second population of oligonucleotides bound to the surface other than through said cleavable linker moiety;
- said first population of oligonucleotides defining an interior area of each feature;
said second population of oligonucleotides defining an edge area of each feature, the edge area substantially surrounding the interior area.
1 Assignment
0 Petitions
Accused Products
Abstract
Oligonucleotide arrays having features that include cleavable oligonucleotides are disclosed, as well as methods of making such arrays. Methods of synthesizing an oligonucleotide on a surface of a substrate are described.
74 Citations
21 Claims
-
1. An oligonucleotide array comprising a substrate having a surface and a plurality of features on the substrate, each feature of the plurality of features comprising A) a first population of oligonucleotides bound via a cleavable linker moiety to the surface, and B) a second population of oligonucleotides bound to the surface other than through said cleavable linker moiety;
- said first population of oligonucleotides defining an interior area of each feature;
said second population of oligonucleotides defining an edge area of each feature, the edge area substantially surrounding the interior area. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
- said first population of oligonucleotides defining an interior area of each feature;
-
12. A method of synthesizing an oligonucleotide on a surface of a substrate, the method comprising,
a) covalently binding a cleavable linker moiety to the surface, the cleavable linker moiety defining an interior area on the surface, b) covalently binding a nucleotide moiety to the surface, the nucleotide moiety defining a combined area on the surface, wherein the combined area includes the interior area and an edge area substantially surrounding the interior area, wherein the nucleotide moiety in the interior area is bound to the surface via the cleavable linker moiety, wherein the nucleotide moiety in the edge area is bound to the surface other than via the cleavable linker moiety; - and
c) repeating b) as necessary to result in synthesis of the oligonucleotide on the surface. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21)
- and
Specification