Assay involving detection and identification with encoded particles
First Claim
1. An assay method comprising:
- (i) providing a planar array of particles randomly-positioned with respect to particle type, where different types of particles are differently encoded by incorporation of an optically detectable characteristic into the particles that permits said differently encoded particles to be distinguished, and wherein said differently encoded particles have different binding molecules attached thereto;
(ii) determining the spatial coordinates of particular particle types within the planar array;
(iii) contacting said array of particles with a solution such that, if a particular type of analyte is present in said solution, said analyte binds to the corresponding binding molecules of said particles and the binding of said analyte is indicated by an optically detectable signal;
(iv) determining the presence of any such bound analyte by determining the presence of said optically detectable signal; and
(v) identifying said optically detectable characteristic of particles having binding molecules bound to an analyte, without moving or separating said particles being interrogated from other particles in the array, by correlating the spatial coordinates of said particles with the location of the optically detectable signals in order to identify said binding molecules bound to an analyte.
3 Assignments
0 Petitions
Accused Products
Abstract
A method and apparatus for the manipulation of colloidal particles and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components.
-
Citations
24 Claims
-
1. An assay method comprising:
-
(i) providing a planar array of particles randomly-positioned with respect to particle type, where different types of particles are differently encoded by incorporation of an optically detectable characteristic into the particles that permits said differently encoded particles to be distinguished, and wherein said differently encoded particles have different binding molecules attached thereto; (ii) determining the spatial coordinates of particular particle types within the planar array; (iii) contacting said array of particles with a solution such that, if a particular type of analyte is present in said solution, said analyte binds to the corresponding binding molecules of said particles and the binding of said analyte is indicated by an optically detectable signal; (iv) determining the presence of any such bound analyte by determining the presence of said optically detectable signal; and (v) identifying said optically detectable characteristic of particles having binding molecules bound to an analyte, without moving or separating said particles being interrogated from other particles in the array, by correlating the spatial coordinates of said particles with the location of the optically detectable signals in order to identify said binding molecules bound to an analyte. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 23, 24)
-
-
19. A method of performing an assay using at least one planar array of particles, the method comprising:
-
forming the planar array with a number of differently encoded particles, on a defined area of a substrate but wherein differently-encoded particles are randomly-positioned within said area, said differently encoded particles having different analyte binding molecules attached thereto, said particle types being encoded by incorporation of an optically detectable characteristic into the particles, that distinguishes said differently encoded particles and the binding molecules attached thereto; determining the spatial coordinates of particular particle types within the planar array; contacting said plurality of particles with a solution such that, if particular analytes are present in said solution, said analytes bind to the corresponding binding molecules attached to said particles to form a paired entity and said analytes carry an optically detectable characteristic; detecting said paired entities; and identifying, the optically detectable characteristic of the particles to which binding molecules are attached which form paired entities by correlating the spatial coordinates of said particles with the spatial coordinates of he optically detectable characteristic in order to identify said binding molecules forming said paired entities. - View Dependent Claims (20, 21, 22)
-
Specification