Optical interrogation system and method for 2-D sensor arrays

• US 7,292,333 B2
• Filed: 04/05/2005
• Issued: 11/06/2007
• Est. Priority Date: 06/24/2003
• Status: Expired due to Fees

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Yuen, Po Ki, Fontaine, Norman H., Mozdy, Eric J.
• Primary Examiner(s): Toatley, Jr.; Gregory J.
• Assistant Examiner(s): Geisel; Kara E.
• Application Number:
  US11/100,199
• Publication Number:
  US 20050236554A1
• Time in Patent Office: 945 Days
• Field of Search: 356/300, 356/445
• US Class Current: 356/300
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide

Abstract

An optical interrogation system and method are described herein that can interrogate a two-dimensional (2D) array of optical sensors (e.g., grating coupled waveguide sensors) located in a 2D specimen plate (e.g., microplate).

Claims

1. An optical interrogation system comprising:

a launch system for directing an array of light beams towards an array of sensors in a two-dimensional specimen plate; and

a receive system including;

a beam expander; and

a detector;

said beam expander for receiving the array of said light beams reflected from the array of sensors, and for reducing the dimension of the array of said light beams and directing each received light beam to a unique region on a detection plane in said detector, wherein said detector is located past an image plane associated with said beam expander for the purpose of measuring response angles in the array of said light beams reflected from the array of sensors in the two-dimensional specimen plate.

2. The optical interrogation system of claim 1, wherein said beam expander is a Kieplerian beam expander that includes:

a plano-convex object lens; and

a positive ocular lens.

3. The optical interrogation system of claim 1, wherein said receive system further includes:

an analyzer for substantially eliminating reflections within the received light beams that are caused by optical components in said launch system.

4. The optical interrogation system of claim 1, wherein said receive system further includes:

a bandpass filter for substantially rejecting stray light that can be detected by said detector.

5. The optical interrogation system of claim 1, wherein said launch system further includes a spherical lens which causes each light beam to illuminate a spot on each sensor in the two-dimensional specimen plate.

6. The optical interrogation system of claim 1, wherein said launch system further includes (a) one or more spherical lenses and one or more cylindrical lenses or (b) one or more line generating lenses which have the effect of causing each light beam to illuminate a line across each sensor in the two-dimensional specimen plate.

7. The optical interrogation system of claim 6, wherein said receive system detects a plurality of optical responses in each received light beam from the line across the illuminated region of each sensor in the two-dimensional specimen plate.

8. The optical interrogation system of claim 7, wherein said receive system spatially divides each received optical response into at least two separate responses, so as to generate separate signal and reference responses that can be subsequently used for self referencing.

9. The optical interrogation system of claim 8, where the signal and reference responses are differenced to produce a referenced response.

10. The optical interrogation system of claim 1, further comprising a processor for analyzing one of the received light beams to determine whether or not a biomolecular binding event occurred, or to determine the rate of binding in a specimen located on one of the sensors in the two-dimensional specimen plate.

11. The optical interrogation system of claim 1, further comprising a processor for analyzing one of the received light beams to determine whether or not there was a cell-based mass transport in a specimen located on one of the sensors in the two-dimensional specimen plate.

36. The optical interrogation system of claim 1, wherein said beam expander is a Galilean beam expander.

12. A receive system comprising:

an object lens;

an image lens; and

a detector;

wherein said object lens receives an array of light beams reflected from a plurality of sensors;

wherein said image lens directs each received light beam to a unique region on a detection plane in said detector; and

wherein said detector is located past an image plane associated with said object and image lenses for the purpose of measuring the response angles from said plurality of sensors.

13. The receive system of claim 12, further comprising an analyzer located between said object lens and said image lens.

14. The receive system of claim 12, further comprising a bandpass filter located between said image lens and said detector.

15. A method for interrogating an array of sensors in a two-dimensional specimen plate, said method comprising the steps of:

using a launch system to direct an array of light beams towards the array of sensors in the two-dimensional specimen plate; and

using a receive system that has a beam expander to receive the array of the light beams reflected from the array of sensors and direct each received light beam to a unique region on a detection plane in a detector, wherein said detector is located past an image plane associated with said beam expander for the purpose of measuring response angles in the array of said light beams reflected from the array of sensors in the two-dimensional specimen plate.

16. The method of claim 15, wherein said detector has a smaller area than the array of sensors.

17. The method of claim 15, further comprising the step of analyzing each received light beam to determine whether or not a biomolecular binding event occurred, or to determine the rate of binding in a specimen located on each sensor.

18. The method of claim 15, further comprising the step of analyzing each received light beam to determine whether or not there was a cell-based mass transport in a specimen located on each sensor.

19. The method of claim 15, further comprising the step of using said launch system which has a spherical lens that causes each light beam to illuminate a spot on each sensor in the two-dimensional specimen plate.

20. The method of claim 15, further comprising the step of using said launch system which has a spherical lens and a cylindrical lens or a line generating lens that causes each light beam to illuminate a line across each sensor in the two-dimensional specimen plate.

21. The method of claim 20, further comprising the step of using said receive system to determine a plurality of optical responses in each received light beam that was illuminated across the line in each sensor in the two-dimensional specimen plate.

22. The method of claim 20, further comprising the step of using said receive system to analyze the optical responses in each received light beam to self-reference each sensor in the two-dimensional specimen plate.

23. The method of claim 15, wherein each sensor has a binding region and a non-binding region.

24. The method of claim 15, wherein each sensor is a grating-coupled waveguide sensor.

25. The method of claim 15, wherein each sensor is associated with a micron-sized deep flow channel.

26. The method of claim 25, wherein each sensor has portions that are associated with parallel flows of fluids in contact with each other in a micron-sized deep flow chamber.

27. The method of claim 15, wherein said two-dimensional specimen plate is a microplate.

28. An optical interrogation system comprising:

a launch system for directing an array of light beams towards an array of sensors in a two-dimensional specimen plate; and

a receive system including;

a beam expander; and

a detector;

said beam expander for receiving the array of said light beams reflected from the array of sensors, and for reducing the dimension of the array of said light beams and directing each received light beam to a unique region on a detection plane in said detector; and

said beam expander is a Keplerian beam expander that includes;

a plano-convex object lens; and

a positive ocular lens.

29. An optical interrogation system comprising:

a launch system for directing an array of light beams towards an array of sensors in a two-dimensional specimen plate; and

a receive system including;

a beam expander; and

a detector;

said beam expander for receiving the array of said light beams reflected from the array of sensors, and for reducing the dimension of the array of said light beams and directing each received light beam to a unique region on a detection plane in said detector; and

said launch system further includes (a) one or more spherical lenses and one or more cylindrical lenses or (b) one or more line generating lenses which have the effect of causing each light beam to illuminate a line across each sensor in the two-dimensional specimen plate.

30. The optical interrogation system of claim 29, wherein said receive system detects a plurality of optical responses in each received light beam from the line across the illuminated region of each sensor in the two-dimensional specimen plate.

31. The optical interrogation system of claim 30, wherein said receive system spatially divides each received optical response into at least two separate responses, so as to generate separate signal and reference responses that can be subsequently used for self referencing.

32. The optical interrogation system of claim 31, where the signal and reference responses are differenced to produce a referenced response.

33. A method for interrogating an array of sensors in a two-dimensional specimen plate, said method comprising the steps of:

using a launch system to direct an array of light beams towards the array of sensors in the two-dimensional specimen plate, wherein said launch system has a spherical lens and a cylindrical lens or a line generating lens that causes each light beam to illuminate a line across each sensor in the two-dimensional specimen plate; and

using a receive system that has a beam expander to receive the array of the light beams reflected from the array of sensors and direct each received light beam to a unique region on a detection plane in a detector.

34. The method of claim 33, further comprising the step of using said receive system to determine a plurality of optical responses in each received light beam that was illuminated across the line in each sensor in the two-dimensional specimen plate.

35. The method of claim 34, further comprising the step of using said receive system to analyze the optical responses in each received light beam to self-reference each sensor in the two-dimensional specimen plate.