Waveguides for performing enzymatic reactions

• US 7,292,742 B2
• Filed: 02/02/2007
• Issued: 11/06/2007
• Est. Priority Date: 05/17/2000
• Status: Expired due to Term

• Current Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Original Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Inventors: Craighead, Harold G., Korlach, Jonas, Turner, Stephen W., Webb, Watt W., Levene, Michael J.
• Primary Examiner(s): HEALY, BRIAN
• Application Number:
  US11/670,906
• Publication Number:
  US 20070134716A1
• Time in Patent Office: 277 Days
• Field of Search: 385/12, 385/14, 385/27, 385/28, 385/123, 385/125, 385/129, 385/130, 385/131, 385/132, 385/146, 435/6, 435/7.1, 435/287.2, 435/91.1, 435/287.1
• US Class Current: 385/12
• CPC Class Codes: B82Y 20/00 Nanooptics, e.g. quantum optics or photonic crystals
• CPC Class Codes: B82Y 35/00 Methods or apparatus for measurement or analysis of nanostructures
• CPC Class Codes: C12Q 1/25 involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: G01N 21/47 Scattering, i.e. diffuse reflection G01N21/25, G01N21/41 take precedence G01N21/55 takes precedence
• CPC Class Codes: G01N 21/6408 with measurement of decay time, time resolved fluorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/65 Raman scattering
• CPC Class Codes: G01N 2333/9015 Ligases (6)
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01Q 60/22 Probes, their manufacture, or their related instrumentation, e.g. holders
• CPC Class Codes: G02B 6/10 of the optical waveguide type G02B6/02, G02B6/24 take precedence; devices or arrangements for the control of light by electric, magnetic, electro-magnetic or acoustic means G02F1/00; transferring the modulation of modulated light G02F2/00; optical logic elements G02F3/00; optical analogue/digital converters G02F7/00
• CPC Class Codes: G02B 6/241 Light guide terminations

Abstract

The present invention is directed to a method and an apparatus for analysis of an analyte. The method involves providing a zero-mode waveguide which includes a cladding surrounding a core where the cladding is configured to preclude propagation of electromagnetic energy of a frequency less than a cutoff frequency longitudinally through the core of the zero-mode waveguide. The analyte is positioned in the core of the zero-mode waveguide and is then subjected, in the core of the zero-mode wave guide, to activating electromagnetic radiation of a frequency less than the cut-off frequency under conditions effective to permit analysis of the analyte in an effective observation volume which is more compact than if the analysis were carried out in the absence of the zero-mode waveguide.

Claims

1. A method for analyzing an enzymatic reaction comprising:

(a) providing a waveguide comprising;

a substrate layer;

a cladding layer disposed upon the substrate layer; and

a core comprising a hole disposed in the cladding layer, wherein the hole is dimensioned such that electromagnetic radiation entering the core provides an effective illumination volume that is substantially internal to the core of said waveguide;

(b) introducing an enzymatic reaction mixture comprising a nucleic acid polymerizing enzyme, a target nucleic acid molecule, a primer sequence, and a nucleotide analog into the core of the waveguide; and

(c) directing excitation radiation at the core to illuminate the enzymatic reaction mixture in the effective illumination volume; and

(d) analyzing the enzymatic reaction in the illumination volume.

2. The method of claim 1, wherein the substrate layer is substantially transparent to electromagnetic energy.

3. The method of claim 2, wherein the electromagnetic radiation enters the core through the substrate layer.

4. The method of claim 1, wherein the waveguide further comprises a superstructure formed on top of the cladding layer having channels to direct the enzymatic reaction mixture into the core for analysis.

5. The method of claim 1, wherein the nucleotide analog is fluorescently labeled.

6. The method of claim 1, wherein the enzymatic reaction mixture comprises more than one type of nucleotide analog.

7. The method of claim 1, wherein at least one of the nucleic acid polymerizing enzyme and the target nucleic acid molecule is immobilized within the core.

8. The method of claim 1, wherein the hole comprises a diameter that is between 1 nm to 100 nm.

9. The method of claim 1, wherein the hole comprises a diameter that is between 30 nm to 100 nm.

10. A method for analyzing an enzymatic reaction comprising:

(a) providing an array of waveguides comprising;

a transparent substrate layer;

a cladding layer disposed upon the substrate layer; and

a plurality of cores, wherein each of the plurality of cores comprises a hole disposed in the cladding layer and wherein the plurality of cores are configured to substantially preclude electromagnetic energy of a frequency less than a cutoff frequency entering the core from propagating longitudinally through said waveguides;

(b) introducing an enzymatic reaction mixture comprising a nucleic acid polymerizing enzyme, a target nucleic acid molecule, a primer sequence, and a nucleotide analog into at least one of the cores of the waveguide; and

(c) directing excitation radiation at the at least one of the cores to illuminate the enzymatic reaction mixture;

(d) analyzing the enzymatic reaction within the core.

11. The method of claim 10, wherein the electromagnetic radiation enters said at least one core through the substrate layer.

12. The method of claim 10, wherein the array further comprises a superstructure formed on top of the cladding layer, the surperstructure comprising one or more channels to direct the enzymatic reaction mixture into at least one of the cores for analysis.

13. The method of claim 10, wherein the nucleotide analog is fluorescently labeled.

14. The method of claim 10, wherein the enzymatic reaction mixture comprises more than one type of nucleotide analog.

15. The method of claim 10, wherein at least one of the nucleic acid polymerizing enzyme and the target nucleic acid molecule is immobilized within the at least one of the cores.

16. The method of claim 10, wherein the hole comprises a diameter that is between 1 nm to 100 nm.

17. The method of claim 10, wherein the hole comprises a diameter that is between 30 nm to 100 nm.