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Waveguides for performing enzymatic reactions

  • US 7,292,742 B2
  • Filed: 02/02/2007
  • Issued: 11/06/2007
  • Est. Priority Date: 05/17/2000
  • Status: Expired due to Term
First Claim
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1. A method for analyzing an enzymatic reaction comprising:

  • (a) providing a waveguide comprising;

    a substrate layer;

    a cladding layer disposed upon the substrate layer; and

    a core comprising a hole disposed in the cladding layer, wherein the hole is dimensioned such that electromagnetic radiation entering the core provides an effective illumination volume that is substantially internal to the core of said waveguide;

    (b) introducing an enzymatic reaction mixture comprising a nucleic acid polymerizing enzyme, a target nucleic acid molecule, a primer sequence, and a nucleotide analog into the core of the waveguide; and

    (c) directing excitation radiation at the core to illuminate the enzymatic reaction mixture in the effective illumination volume; and

    (d) analyzing the enzymatic reaction in the illumination volume.

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