Detector arrangement based on surfaces plasmon resonance

• US 7,295,320 B2
• Filed: 11/30/2004
• Issued: 11/13/2007
• Est. Priority Date: 05/31/2002
• Status: Expired due to Fees

• Current Assignee: Gyros Patent AB (Mesa Laboratories Inc.)
• Original Assignee: Gyros AB (Mesa Laboratories Inc.)
• Inventors: Ostlin, Henrik, Agren, Tomas, Ljungstrom, Magnus, Eriksson, Lars
• Primary Examiner(s): Toatley, Jr.; Gregory J.
• Assistant Examiner(s): Valentin, II; Juan D
• Application Number:
  US10/999,532
• Publication Number:
  US 20050179901A1
• Time in Patent Office: 1,078 Days
• Field of Search: None
• US Class Current: 356/445
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2300/0806 Standardised forms, e.g. compact disc [CD] format
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: G01N 21/07 Centrifugal type cuvettes G01N21/09 takes precedence
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658
• CPC Class Codes: G01N 21/554 detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance
• CPC Class Codes: G01N 35/00069 whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk

Abstract

A detector arrangement, which comprises a rotatable microfluidic disc and a spectrophotometric detector unit. The arrangement is characterized in that the detector unit is based on surface plasmon resonance (SPR) and is capable of measuring an analyte within the detection microcavities, each of which is part of a microchannel structure.

A microfluidic disc having an axis of symmetry and comprising microchannel structures, each of which has an upstream functional part that is at a shorter radial position than a downstream functional part. The disc is characterized in that there are detection microcavities (DMCs) in at least a part of said microchannel structures, and that each of said DMCs has an SPR surface on an inner wall and a detection detection window extending from the SPR surface to the surface of the disc.

The use of the detector arrangement and microfluidic disc described above for determining: a) if the content in one or more of the DMCs is a liquid or a solid state or a gas, and/or b) a feature of an analyte that may be present in a liquid which is present in one or more of the DMCs.

Claims

1. A detector arrangement comprising:

a) a rotary member having an axis of rotation, said member comprises;

(i) one or more microfluidic discs, each disc comprises a plurality of essentially equal and enclosed microchannel structures which are designed for permitting liquid transport by centrifugal force created by spinning the disc around the axis of rotation, and a first plurality of surface plasmon resonance measuring cells (SPR-MCs), each SPR-MCs comprises a detection microcavity (DMC), wherein an SPR surface in an inner wall of the DMC and at least a part (P1) of a detection window (DW) that stretches from the SPR surface to an outer surface of the disc, and said outer surface of P1 for all SPR-MCs are at the same radial distances

(ii) a rotatable disc holder comprising the remaining part (P2) of each of the DWs,

b) a non-rotatable SPR detector comprising

(i) a light source (LS), and

(ii) a light detecting subunit (LDS),

c) a beam path comprising

(i) an incident beam path (ibp) going from LS to one of the SPR surfaces via the DW associated with this SPR surface, and

(ii) a reflected beam path (rpb) going from the same SPR surface to LDS via said DW, the SPR-MCs being interchangeable by rotating said rotatory member.

2. The arrangement of claim 1, wherein each DW is fully integrated with each disc.

3. The arrangement of claim 1, wherein the DWs and the disc holder are present at the same side of the microfluidic disc.

4. The arrangement of claim 3, wherein

(a) P1 and P2 of each of the DWs are connected to each other via an opto interface, and

(b) ibp and rbp are capable of passing through both of said parts for each SPR-MC by rotating the rotary member.

5. The arrangement of claim 4, wherein said opto interface for each DW is present in an opto interface plate that is placed between the microfluidic disc and the disc holder.

6. The arrangement of claim 4, wherein

(a) P2 for each DW is part of a separate plate that is a part of the disc holder, and

(b) the disc is resting on top of this plate, said separate plate is made of glass.

7. The arrangement of claim 3, wherein ibp reaches and/or rpb path for each SPR-MC leaves the DW via one or more openings in the disc holder.

8. The detector arrangement of claim 1, wherein the DWs and the disc holder are on the opposite sides of the microfluidic disc.

9. The detector arrangement of claim 1, wherein said SPR detector and said rotary member are laterally movable relative to each other.

10. The arrangement of claim 9, wherein each microfluidic disc comprises a second plurality of SPR-MCs that are located at radial distances that are different from the radial distances of said first plurality.

11. The arrangement of claim 1, wherein the breadth of a light beam passing through the beam paths is less than the dimension of each of the SPR surfaces and its underlying detection chamber.

12. The arrangement claim 1, wherein at least a part of the SPR-MCs including the SPR surfaces and detection windows is arranged annularly as one or more concentric circles around the axis of symmetry.

13. The arrangement of claim 1, wherein at least a part of the SPR-MCs including the SPR surfaces and detection windows is in a spoke arrangement.

14. The arrangement of claim 1, wherein P1 for each of the DWs is made in plastic material having a refractive index within the range of 1.45-1.55.

15. The detector arrangement of claim 1, wherein each of the microcavities are part of a microchannel structure that may be the same or different for different microcavities.

16. The detector arrangement of claim 1, wherein the surfaces at the entrance and the exit for ibp and rpb, respectively, are essentially perpendicular to the optical axis of the incident and reflected light.

17. The detector arrangement of claim 1, wherein each DW is delineated by a prism surface at each of the entrance and exit of light.

18. The detector arrangement of claim 1, wherein subpressure retains the microfluidic disc in the disc holder.