Method and a device for monitoring a medical microsample in the flow measuring cell of an analyzer

• US 7,297,241 B2
• Filed: 08/22/2003
• Issued: 11/20/2007
• Est. Priority Date: 08/23/2002
• Status: Active Grant

• Current Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: Huemer, Herfried, Hajnsek, Martin, Kontschieder, Heinz
• Primary Examiner(s): Nguyen; Nam
• Assistant Examiner(s): Vathyam; Surekha
• Application Number:
  US10/646,109
• Publication Number:
  US 20060011493A1
• Time in Patent Office: 1,551 Days
• Field of Search: 204409-412, 204/401, 204/403.1, 205/775, 205/792
• US Class Current: 204/403.01
• CPC Class Codes: G01N 2035/1018 Detecting inhomogeneities, e.g. foam, bubbles, clots
• CPC Class Codes: G01N 27/10 Investigation or analysis specially adapted for controlling or monitoring operations or for signalling

Abstract

The invention relates to a method and device for the monitoring of a medical microsample in the flow measuring cell of an analyzer with regard to position and absence of bubbles by means of an alternating voltage applied to the measuring cell, the measuring cell being provided with a multitude of electrode systems placed one behind the other, each system comprising a number of single electrodes for measuring a substance contained in the microsample by means of a measurement voltage which essentially is a DC voltage. To monitor the exact position of the microsample and/or to detect air bubbles in the area of each electrode system, the alternating voltage and the measurement voltage are simultaneously and directly applied to the single electrodes of the corresponding electrode system, and the measured AC component respectively the measured impedance gives a measure for the position of the microsample and the absence of bubbles.

Claims

1. A device for monitoring a microsample in a flow measuring cell of an analyzer comprising:

at least one electrode system comprising at least two single electrodes positioned within said flow measuring cell, wherein

said electrode system is configured for measurement of at least one substance contained in the microsample by application of a measurement voltage which is essentially a DC voltage, and detection of absence of bubbles and/or positioning of said microsample by application of an alternating voltage to said flow measuring cell,

both the alternating voltage and the measurement voltage are simultaneously and directly applied to the single electrodes of the respective electrode system, and

the measured AC component or the measured impedance provides a measure for the position of the microsample and/or the absence of bubbles; and

a circuit for producing the voltages to be applied to the single electrodes, which circuit has a summation point at which the alternating voltage for the purpose of monitoring the microsample with regard to position and/or absence of bubbles is superposed on the DC voltage serving as measurement voltage.

2. A device according to claim 1, wherein an electrode system includes a working electrode and a reference electrode, both electrodes serving as electrical contacts for measuring the impedance between working electrode and reference electrode.

3. A device according to claim 1, wherein an electrode system includes a working electrode, a counter-electrode and a reference electrode, the working electrode and the counter-electrode serving as electrical contacts for measuring the impedance between working electrode and counter-electrode.

4. A device according to claim 3, wherein the working electrode is positioned in front of the reference electrode, and the counter-electrode is positioned behind the reference electrode in flow direction of the microsample.

5. A device according to claim 3, wherein counter-electrodes are placed both in front of and behind the working electrode in flow direction of the microsample, both counter-electrodes being electrically short-circuited.

6. A device according to claim 3, wherein the counter-electrode and the working electrode are positioned opposite each other in the measuring cell.

7. A device according to claim 1, wherein the summation point is connected with the inverting input terminal of an operational amplifier.

8. A device according to claim 1, wherein each electrode system is provided with a device for measuring impedance, which is configured as a circuit for superposing an alternating voltage on a DC voltage.

9. A method for monitoring a microsample in a flow measuring cell of an analyzer comprising:

providing an analyzer including a flow measuring cell and a device comprising at least one electrode system, each said system comprising at least two single electrodes;

introducing a microsample into said flow measuring cell, said microsample passing said electrode system;

measuring at least one substance contained in the microsample by applying a measurement voltage to said flow measuring cell, which said measurement voltage is essentially a DC voltage; and

detecting the absence of bubbles and/or position of said microsample in an area of said at least one electrode system by applying an alternating voltage to said flow measuring cell simultaneous with said measurement voltage via two single electrodes of said at least one electrode system, and detecting the AC component or impedance.

10. A method according to claim 9, wherein the microsample in the flow measuring cell is moved along until a predetermined value for impedance or conductance is obtained, which indicates that the microsample is positioned precisely in the area of the respective electrode system.

11. A method according to claim 9, wherein the sample position and/or absence of bubbles of the microsample are determined in the area of each electrode system.