Simultaneous multianalyte electrochemical assay based on spatial resolution

• US 7,297,312 B2
• Filed: 03/12/1999
• Issued: 11/20/2007
• Est. Priority Date: 03/16/1998
• Status: Expired due to Term

• Current Assignee: University of Cincinnati
• Original Assignee: University of Cincinnati
• Inventors: Heineman, William R., Ding, Ying, Halsall, Brian
• Primary Examiner(s): GABEL, GAILENE
• Application Number:
  US09/268,437
• Publication Number:
  US 20010029048A1
• Time in Patent Office: 3,175 Days
• Field of Search: 422/82.01, 422/82.02, 422/82.03, 422/50, 422/68.1, 422/90, 422/98, 435/6, 435/7.1, 435/7.9, 435/7.92, 435/93, 435/94, 435/95, 435/971, 435/973, 435/176, 435/285.2, 435/288.4, 436/525, 436/806, 436/524, 436/149
• US Class Current: 422/82.01
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10S 435/971 Capture of complex after antigen-antibody reaction
• CPC Class Codes: Y10S 435/973 Simultaneous determination of more than one analyte

Abstract

A simultaneous multianalyte electrochemical assay includes a cell which has a surface and the surface includes analyte binding sites i.e., antibodies or antigens on a solid phase at distinct separate locations. Separate working electrodes are located within proximity to these separate locations. Enzyme labeled antibodies or antigens depending on the assay format are added and the enzyme reaction product measured, by simultaneous amperometric measurement with the independent electrode in each area. The electrodes are spatially separated from adjacent analytes so that a measurement can be taken before cross-interference due to diffusion of product from adjacent analyte areas.

Claims

1. A simultaneous electrochemical assay device comprising a cell adapted to receive a sample, said cell having a surface having a plurality of analyte binding areas, each of said analyte binding areas having a different specific analyte binding substrate;

and a plurality of working electrodes adapted to quantitatively measure enzymatic reaction product, each working electrode adjacent to one analyte binding area and separated from the nearest adjacent analyte binding area by a distance and a common reference electrode for said plurality of working electrodes wherein said device does not have means to mix a sample in said cell.

2. The device claimed in claim 1 wherein said binding substrates each comprise a plurality of different analyte specific proteins.

3. The device claimed in claim 1 wherein said binding substrates each comprise a different antigen.

4. The device claimed in claim 1 wherein said binding substrate comprises a different antibody.

5. The device claimed in claim 1 further comprising at least one auxiliary electrode in said cell.

6. The assay device claimed in claim 1 wherein said device has a common reference electrode for said plurality of working electrodes.