Substrates for isolating, reacting and microscopically analyzing materials
First Claim
1. A device constructed for immobilizing material for exposure to liquid during an assay, the device comprising a polymer layer on a rigid support, the layer having an outer deposit-receiving region that has binding properties for a bio-material, wherein the layer comprises nitrocellulose in the form of a coating of substantially solid film of thickness less than about 1 micron and a deposit of the biomaterial is immobilized on the deposit-receiving region.
9 Assignments
0 Petitions
Accused Products
Abstract
An immobilizing device for biological material comprises a rigid support (12) carrying a substrate layer (20, 20′) of polymer having biological immobilizing properties, e.g. for amino and nucleic acids. Substantially solid ultra-thin substrate layers (20′) having a thickness less than about 5 micron, preferably between about 0.1 and 0.5 micron, and micro-porous, ultra-thin substrate layers (20′) having a thickness less than about 5 micron, preferably less than 3 micron, 2 or 1 micron are shown, which may be segmented by isolating moats M. The substrate layer is on a microscope slide (302), round disc (122), bio-cassette, at the bottom of a well of a multiwell plate, and as a coating inside a tube. Fluorescence or luminescence intensity and geometric calibration spots (420) are shown. Reading is enhanced by the intensity calibration spots. (420) to enable normalization of readings under uneven illumination conditions, as when reading by dark field, side illumination mode. The reference spots are shown being printed simultaneously with printing an array of biological spots or with the same equipment. Methods of forming layers of the device include controlled drawing from a bath of coating composition and drying, and spinning of C-D shaped substrates. Post-forming treatment is shown by corona treatment and radiation. Adherent metal oxides (14), silica-based materials and other materials are used to unite layers of the composite. In multi-well plates the oxide promotes joining of a bottom plate (95, 95′) and upper, well-defining structure (94) of dissimilar material. The oxides (14) also provide beneficial opacity to prevent light entering the glass support, for applying potential to the substrate, etc.
32 Citations
78 Claims
- 1. A device constructed for immobilizing material for exposure to liquid during an assay, the device comprising a polymer layer on a rigid support, the layer having an outer deposit-receiving region that has binding properties for a bio-material, wherein the layer comprises nitrocellulose in the form of a coating of substantially solid film of thickness less than about 1 micron and a deposit of the biomaterial is immobilized on the deposit-receiving region.
- 38. A method of forming a device constructed for immobilizing material for exposure to liquid during an assay, the device comprising a polymer layer on a rigid support, the layer having an outer deposit-receiving region that has binding properties for a bio-material, wherein the layer comprises nitrocellulose in the form of a coating of substantially solid film of thickness less than about 1 micron and a deposit of the biomaterial is immobilized on the deposit-receiving region, the method including applying directly or indirectly to said rigid support a coating composition comprising nitrocellulose and at least one solvent while maintaining forming conditions to produce a substantially solid film coating of nitrocellulose of thickness less than about 1 micron.
- 42. A method of conducting an assay including providing a device constructed for immobilizing material for exposure to liquid during an assay, the device comprising a polymer layer on a rigid support, the layer having an outer deposit-receiving region that has binding properties for a bio-material, wherein the layer comprises nitrocellulose in the form of a coating of substantially solid film of thickness less than about 1 micron and a deposit of the bio-material is immobilized on the deposit-receining region, the deposit comprising an array of spots of biomaterial, conducting an assay which tags at least some of the spots with a fluorescent label, and, after washing the array, reading the array by optically stimulating emission from the fluorescent labels and fluorescent detection of the stimulated emission.
- 72. The method of claom 41 in which the rigid support comprises a glass body bearing a layer of adhesion promoting substance that defines the support surface.
Specification