Scaffold and method for implanting cells

• US 7,299,805 B2
• Filed: 06/06/2003
• Issued: 11/27/2007
• Est. Priority Date: 06/07/2002
• Status: Active Grant

• Current Assignee: P TECH, LLC
• Original Assignee: MarcTec LLC
• Inventors: Bonutti, Peter M.
• Primary Examiner(s): Snow; Bruce
• Assistant Examiner(s): MILLER, CHERYL L
• Application Number:
  US10/457,100
• Publication Number:
  US 20040078073A1
• Time in Patent Office: 1,635 Days
• Field of Search: 623/1.1, 623/23.72, 623/23.64, 623/23.65, 623/903, 623/921, 623/916, 623/920, 128/898
• US Class Current: 128/898
• CPC Class Codes: A61F 2/0077 Special surfaces of prostheses, e.g. for improving ingrowth A61F2/30767 takes precedence
• CPC Class Codes: A61F 2/02 Prostheses implantable into the body closure means for urethra or rectum or for artificial body openings therefor A61F2/0004
• CPC Class Codes: A61F 2/28 Bones joints A61F2/30
• CPC Class Codes: A61F 2/4601 for introducing bone substitute, for implanting bone graft implants or for compacting them in the bone cavity
• CPC Class Codes: A61F 2002/2817 Bone stimulation by chemical reactions or by osteogenic or biological products for enhancing ossification, e.g. by bone morphogenetic or morphogenic proteins [BMP] or by transforming growth factors [TGF]
• CPC Class Codes: A61F 2002/2835 Bone graft implants for filling a bony defect or an endoprosthesis cavity, e.g. by synthetic material or biological material
• CPC Class Codes: A61F 2210/0004 bioabsorbable
• CPC Class Codes: A61L 24/0015 Medicaments; Biocides
• CPC Class Codes: A61L 24/08 Polysaccharides A61L24/043 takes precedence
• CPC Class Codes: C12N 2501/10 Growth factors
• CPC Class Codes: C12N 2501/105 Insulin-like growth factors [IGF]
• CPC Class Codes: C12N 2501/11 Epidermal growth factor [EGF]
• CPC Class Codes: C12N 2501/135 Platelet-derived growth factor [PDGF]
• CPC Class Codes: C12N 2501/155 Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
• CPC Class Codes: C12N 2513/00 3D culture
• CPC Class Codes: C12N 2533/54 Collagen; Gelatin
• CPC Class Codes: C12N 2533/90 Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
• CPC Class Codes: C12N 5/0062 General methods for three-dimensional culture
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0654 Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
• CPC Class Codes: C12N 5/0691 : Vascular smooth muscle cells; 3D culture thereof, e.g. models of blood vessels
• CPC Class Codes: Y10S 623/92 : Method or apparatus for preparing or treating prosthetic

Abstract

An improved method of implanting cells in the body of a patient includes positioning viable cells on a support structure. One or more blood vessels may be connected with the support structure to provide a flow of blood through the support structure. A support structure may be positioned at any desired location in a patient'"'"'s body. The support structure may be configured to replace an entire organ or a portion of an organ. An organ or portion of an organ may be removed from a body cells and/or other tissue is removed to leave a collagen matrix support structure having a configuration corresponding to the configuration of the organ or portion of an organ. Alternatively, a synthetic support structure may be formed. The synthetic support structure may have a configuration corresponding to a configuration of an entire organ or only a portion of an organ.

Claims

1. A method of implanting cells into a body of a patient, said method comprising the steps of:

providing a support structure,

harvesting a polysaccharide-based modified biofilm from bacteria,

attaching viable cells for implantation to the support structure with the polysaccharide-based modified biofilm, and

connecting one portion of a blood vessel in the patient'"'"'s body with a first portion of the support structure, and connecting another portion of a blood vessel in the patient'"'"'s body with a second portion of the support structure.

2. A method as set forth in claim 1 further including the step of positioning the support structure in an organ in the body of the patient with the viable cells disposed on the support structure.

3. A method as set forth in claim 1 further including the step of conducting a flow of blood from the one portion of a blood vessel in the patient'"'"'s body through the support structure to the other portion of the blood vessel in the patient'"'"'s body.

4. A method as set forth in claim 1 wherein said step of connecting one portion of a blood vessel with a first portion of the support structure includes connecting an artery with the first portion of the support structure, said step of connecting another portion of a blood vessel with a second portion of the support structure includes connecting a vein with the second portion of the support structure.

5. A method as set forth in claim 1 wherein said step of connecting one portion of a blood vessel with a first portion of the support structure includes connecting a first portion of an artery with the first portion of the support structure, said step of connecting another portion of a blood vessel with a second portion of the support structure includes connecting a second portion of an artery with the second portion of the support structure.

6. A method as set forth in claim 1 wherein said step of connecting one portion of a blood vessel with a first portion of the support structure includes connecting a first portion of a vein with the first portion of the support structure, said step of connecting another portion of a blood vessel with a second portion of the support structure includes connecting a second portion of a vein with the second portion of the support structure.

7. A method as set forth in claim 1 wherein said step of connecting one portion of a blood vessel with a first portion of the support structure includes connecting a first blood vessel with the first portion of the support structure, said step of connecting another portion of a blood vessel with the second portion of the support structure includes connecting a second blood vessel with the second portion of the support structure.

8. A method as set forth in claim 1 wherein said step of providing a support structure includes providing a support structure with first and second conduits extending from the support structure, said step of connecting one portion of a blood vessel in the patient'"'"'s body with the first portion of the support structure includes connecting the first conduit with an artery in the patient'"'"'s body, said step of connecting another portion of a blood vessel in the patient'"'"'s body with the second portion of the support structure includes connecting the second conduit with a vein in the patient'"'"'s body.

9. A method as set forth in claim 1 wherein said step of providing a support structure includes providing a support structure with first and second conduits extending from the support structure, said step of connecting one portion of a blood vessel in the patient'"'"'s body with the first portion of the support structure includes connecting the first conduit with a portion of an artery in the patient'"'"'s body, said step of connecting another portion of a blood vessel in the patient'"'"'s body includes connecting the second conduit with a portion of an artery in the patient'"'"'s body.

10. A method as set forth in claim 1 wherein said step of providing a support structure includes providing a support structure with first and second conduits extending from the support structure, said step of connecting one portion of a blood vessel in the patient'"'"'s body with the first portion of the support structure includes connecting the first conduit with a portion of a vein the patient'"'"'s body, said step of connecting another portion of a blood vessel in the patient'"'"'s body with the second portion of the support structure includes connecting the second conduit with a portion of a vein in the patient'"'"'s body.

11. A method as set forth in claim 1 wherein said step of providing a support structure includes providing a support structure with first and second conduits extending from the support structure, said step of connecting one portion of a blood vessel in the patient'"'"'s body with the first portion of the support structure includes connecting the first conduit with a first blood vessel in the patient'"'"'s body, said step of connecting another portion of a blood vessel in the patient'"'"'s body with the second portion of the support structure includes connecting the second conduit with a second blood vessel in the patient'"'"'s body.

12. A method as set forth in claim 1 wherein said step of providing a support structure includes providing a support structure with first and second openings, said step of connecting one portion of a blood vessel in the patient'"'"'s body with the first portion of the support structure includes moving the one portion of a blood vessel into the first opening, said step of connecting another portion of a blood vessel in the patient'"'"'s body with the second portion of the support structure includes moving the other portion of a blood vessel into the second opening.

13. A method as set forth in claim 12 further including the steps of at least partially sealing a joint between the one portion of a blood vessel and the first opening, and at least partially sealing a joint between the other portion of a blood vessel and the second opening.

14. A method as set forth in claim 1 wherein said step of providing a support structure includes providing a support structure with first and second openings, said step of connecting one portion of a blood vessel in the patient'"'"'s body with the first portion of the support structure includes moving a portion of an artery into the first opening, said step of connecting another portion of a blood vessel in the patient'"'"'s body with the second portion of the support structure includes moving a portion of a vein into the second opening.

15. A method as set forth in claim 14 further including the steps of at least partially sealing a joint between the artery and the first opening, and at least partially sealing a joint between the vein and the second opening.

16. A method as set forth in claim 1 wherein said step of providing a support structure includes providing a support structure with first and second openings, said step of connecting a portion of a blood vessel in a patient'"'"'s body with the first portion of the support structure includes moving a portion of an artery into the first opening, said step of connecting another portion of a blood vessel in the patient'"'"'s body with the second portion of the support structure includes moving a portion of an artery into the second opening.

17. A method as set forth in claim 1 wherein said step of providing a support structure includes providing a support structure with first and second openings, said step of connecting a portion of a blood vessel in a patient'"'"'s body with the first portion of the support structure includes moving a portion of a vein into the first opening, said step of connecting another portion of a blood vessel in the patient'"'"'s body with the second portion of the support structure includes moving a portion of a vein into the second opening.

18. A method as set forth in claim 1 wherein said steps of connecting one portion of a blood vessel in the patient'"'"'s body with a first portion of the support structure and connecting another portion of a blood vessel in the patient'"'"'s body with a second portion or the support structure are performed with the first and second portions of the support structure disposed on opposite sides of the support structure.

19. A method as set forth in claim 1 wherein said steps of connecting one portion of a blood vessel in the patient'"'"'s body with a first portion of the support structure and connecting another portion of a blood vessel in the patient'"'"'s body with a second portion of the support structure are performed with the first and second portions of the support structure disposed on the same side of the support structure.

20. A method as set forth in claim 1 wherein said step of providing a support structure includes providing a support structure containing a barrier, said method further includes directing blood flow from the first portion of the support structure to the second portion of the support structure with the barrier.

21. A method as set forth in claim 1 wherein said step of providing a support structure includes providing a support structure with outer sides which retard a flow of blood through the outer sides of the support structure during a flow of blood through the support structure.

22. A method as set forth in claim 1 further including the step of connecting a portion of a blood vessel in a patient'"'"'s body with a third portion of the support structure, conducting a flow of blood to the support structure through the first portion of the support structure, conducting a flow of blood from the support structure through the second portion of the support structure, and conducting a flow of blood either to or from the support structure through the third portion of the support structure.

23. A method as set forth in claim 1 wherein said step of attaching viable cells on the support structure includes attaching viable progenitor cells on a three dimensional support structure.

24. A method as set forth in claim 1 further including the steps of removing a portion of an organ from the patient'"'"'s body, and connecting the support structure with the organ at a location where the portion of an organ was removed.

25. A method as set forth in claim 1 wherein said step of providing a support structure includes providing a support structure having a surface area with a configuration corresponding to a configuration of a portion of a surface area of an organ in the patient'"'"'s body, removing a portion of the organ on which the portion of a surface area of the organ is disposed, positioning the support structure in engagement with the organ at a location where the portion of the organ was removed, said step of positioning the support structure in engagement with the organ includes aligning the surface area on the support structure with a surface area on the organ.

26. A method as set forth in claim 1 wherein said step of providing a support structure includes removing an organ from a body, and removing cells from the organ which is removed from the body to leave a support structure having a configuration corresponding to the configuration of the organ, said step of attaching viable cells on the support structure includes attaching the viable cells on the support structure having a configuration corresponding to the configuration of the organ which is removed from a body.

27. A method as set forth in claim 26 wherein said step of removing an organ from a body includes removing the organ from a cadaver.

28. A method as set forth in claim 26 wherein said step of removing an organ from a body includes removing an organ from the body of the patient.

29. A method as set forth in claim 28 wherein said step of attaching viable cells on the support structure having a configuration corresponding to the configuration of the organ which is removed from a body includes attaching a first type of viable cell on a first portion of the support structure and attaching a second type of viable cell on a second portion of the support structure.

30. A method as set forth in claim 29 wherein said step of attaching a first type of cell on a first portion of the support structure includes attaching endocrine cells on the first portion of the support structure and said step of attaching a second type of cell on a second portion of the support structure includes attaching stromal cells on the second portion of the support structure.

31. A method as set forth in claim 29 wherein said step of attaching a first type of cell on a first portion of the support structure includes attaching endothelial cells on the first portion of the support structure and said step of attaching a second type of cell on a second portion of the support structure includes attaching stromal cells on the second portion of the support structure.

32. A method as set forth in claim 26 wherein said step of removing an organ from a body includes removing an organ from a body of a living human.

33. A method as set forth in claim 26 wherein said step of removing an organ from a body includes removing an organ from a body of an animal.

34. A method as set forth in claim 1 wherein said step of providing a support structure includes removing a portion of an organ from a body, and removing cells from the portion of an organ which is removed from the body to leave a support structure having a configuration corresponding to the configuration of the portion of an organ, said step of positioning viable cells on the support structure includes positioning the viable cells on the support structure having a configuration corresponding to the configuration of the portion of an organ which is removed from a body.

35. A method as set forth in claim 34 wherein said step of removing a portion of an organ from a body includes removing the portion of an organ from a cadaver.

36. A method as set forth in claim 34 wherein said step of removing a portion of an organ from a body includes removing a portion of an organ from the body of the patient.

37. A method as set forth in claim 34 wherein said step of removing a portion of an organ from a body includes removing a portion of an organ from a body of a living human.

38. A method as set forth in claim 34 wherein said step of removing a portion of an organ from a body includes removing a portion of an organ from a body of an animal.

39. A method as set forth in claim 34 wherein said step of positioning viable cells on the support structure having a configuration corresponding to the configuration of the portion of an organ which is removed from a body includes positioning a first type of cell on a first portion of the support structure and positioning a second type of cell on a second portion of the support structure.

40. A method as set forth in claim 39 wherein said step of positioning a first type of cell on a first portion of the support structure includes positioning endocrine cells on the first portion of the support structure and positioning stromal cells on the second portion of the support structure.

41. A method as set forth in claim 39 wherein said step of positioning a first type of cell on a first portion of the support structure includes positioning endothelial cells on the first portion of the support structure and fibroblasts on the second portion of the support structure.

42. A method as set forth in claim 1 wherein said step of attaching viable cells on the support structure includes attaching at least two different types of cells on the support structure.

43. A method as set forth in claim 1 wherein said step of providing a support structure includes shaping the support structure to have a configuration corresponding to a configuration of an organ in a human body.

44. A method as set forth in claim 1 wherein said step of providing a support structure includes shaping the support structure to have a configuration corresponding to a configuration of at least a portion of an organ in a human body.

45. A method as set forth in claim 1 wherein said step of providing support structure includes spin molding the support structure to have a configuration corresponding to a configuration of at least a portion of an organ in a human body.

46. A method as set forth in claim 1 wherein said step of proving a support structure includes weaving a three dimensional support structure.

47. A method as set forth in claim 1 wherein said step of providing a support structure includes providing a pattern having a configuration corresponding to a configuration of at least a portion of an organ in a human body, shaping mold material against the pattern, separating the pattern and mold material to leave a mold cavity, and shaping material in the mold cavity to form the support structure.

48. A method as set forth in claim 1 wherein said step of attaching viable cells on the support structure includes attaching mesenchymal cells on the support structure.

49. A method as set forth in claim 1 wherein said step of attaching viable cells on the support structure includes attaching fetal cells on the support structure.

50. A method as set forth in claim 1 wherein said step of attaching viable cells on the support structure includes attaching embryonal cells on the support structure.

51. A method as set forth in claim 1 wherein said step of attaching viable cells on the support structure includes attaching endocrine cells on the support structure.

52. A method as set forth in claim 1 wherein said step of attaching viable cells on the support structure includes attaching stromal cells on the support structure.

53. A method as set forth in claim 1 wherein said step of attaching viable cells on the support structure includes attaching fibroblasts on the support structure.

54. A method as set forth in claim 1 wherein said step of attaching viable cells on the support structure includes attaching endothelial cells on the support structure.

55. A method as set forth in claim 1 wherein said step of providing a support structure includes providing a tubular support structure, said step of connecting one portion of a blood vessel in the patient'"'"'s body with a first portion of the support structure includes connecting the one portion of a blood vessel with a first end of the tubular support structure, said step of connecting another portion of a blood vessel in the patient'"'"'s body with a second portion of the support structure includes connecting another portion of a blood vessel with a second end of the tubular support structure.

56. A method as set forth in claim 55 wherein said step of attaching viable cells on the support structure includes attaching endothelial cells on the support structure.

57. A method as set forth in claim 1 wherein said step of providing a support structure includes the step of providing a support structure formed of a biodegradable material.

58. A method as set forth in claim 1 wherein said step of providing a support structure includes providing a support structure formed of a nonbiodegradable material.

59. A method as set forth in claim 1 wherein said step of providing a support structure includes providing a support structure formed of collagen.

60. A method as set forth in claim 1 wherein said step of attaching viable cells on the support structure includes attaching osteoblast on the support structure.

61. A method as set forth in claim 1, which further comprises sterilizing the polysaccharide-based modified biofilm before attaching the biofilm to the support structure.

62. A method as set forth in claim 1 wherein the polysaccharide-based modified biofilm is a glue-like substance.

63. A method as set forth in claim 1 wherein the polysaccharide-based modified biofilm provides initial attachment of viable cells to the support structure.

64. A method as set forth in claim 1 wherein attaching viable cells includes applying the polysaccharide-based modified biofilm to the support structure intracorporeally.

65. A method of implanting cells into a body of a patient, said method comprising the steps of providing a plurality of support structures, preparing a polysaccharide-based modified biofilm from bacteria;

attaching viable cells for implantation on each of the support structures with the polysaccharide-based modified biofilm, forming a plurality of openings in an organ in the patient'"'"'s body, and positioning the plurality of support structures in the plurality of openings in the organ in the patient'"'"'s body with the viable cells disposed on the support structures.

66. A method of implanting cells into a body of a patient, said method comprising the steps of removing an organ from a body, removing cells from the organ which is removed from the body to leave a support structure having a configuration corresponding to the configuration of the organ, preparing a polysaccharide-based modified biofilm from bacteria positioning viable cells for implantation on the support structure having a configuration corresponding to the configuration of the organ which is removed from a body, attaching the viable cells to the support structure with the polysaccharide-based modified biofilm, and positioning the support structure in the patient'"'"'s body with the viable cells disposed on the support structure.

67. A method as set forth in claim 66 wherein said step of removing an organ from a body includes removing an organ from a cadaver.

68. A method as set forth in claim 66 wherein said step of removing an organ from a body includes removing an organ from the body of a patient.

69. A method as set forth in claim 66 wherein said step of removing an organ from a body includes removing an organ from a body of an animal.

70. A method as set forth in claim 66 wherein said step of removing an organ from a body includes severing first and second blood vessels connected with the organ, said step of positioning the support structure in the patient'"'"'s body includes connecting one portion of a blood vessel in the patient'"'"'s body with a portion of the first severed blood vessel connected with the organ and connecting another portion of a blood vessel in the patient'"'"'s body with a portion of the second severed blood vessel connected with the organ.

71. A method as set forth in claim 66 wherein said step of positioning viable cells on the support structure having a configuration corresponding to a configuration of the organ which is removed from a body includes positioning a first type of viable cell on a first portion of the support structure and positioning a second type of viable cell on a second portion of the support structure.

72. A method as set forth in claim 71 wherein said step of positioning a first type of cell on a first portion of the support structure includes positioning endocrine cells on the first portion of the support structure and positioning stromal cells on a second portion of the support structure.

73. A method as set forth in claim 71 wherein said step of positioning a first type of cell on a first portion of the support structure includes positioning renal cells on the first portion of the support structure and said step of positioning a second type of cell on a second portion of the support structure includes positioning stromal cells on the second portion of the support structure.

74. A method as set forth in claim 71 wherein said step of positioning a first type of cell on a first portion of the support structure includes positioning endothelial cells on the first portion of the support structure and said step of positioning a second type of cell on a second portion of the support structure includes positioning stromal cells on the second portion of the support structure.

75. A method as set forth in claim 66 wherein said step of positioning viable cells on the support structure includes positioning progenitor cells on the support structure.

76. A method as set forth in claim 66 wherein said step of positioning viable cells on the support structure includes positioning endothelial cells on the support structure.

77. A method as set forth in claim 66 wherein said step of positioning viable cells on the support structure includes positioning fibroblasts on the support structure.

78. A method as set forth in claim 66 wherein said step of positioning viable cells on the support structure includes positioning stromal cells on the support structure.

79. A method as set forth in claim 66 wherein said step of positioning viable cells on the support structure includes positioning endocrine cells on the support structure.

80. A method as set forth in claim 66 wherein said step of positioning viable cells on the support structure includes positioning embryonal cells on the support structure.

81. A method as set forth in claim 66 wherein said step of positioning viable cells on the support structure includes positioning fetal cells on the support structure.

82. A method as set forth in claim 66 wherein said step of positioning viable cells on the support structure includes positioning mesenchymal cells on the support structure.

83. A method as set forth in claim 66 wherein said step of removing from the organ includes removing cells from the organ while at least partially maintaining a collagen matrix of the organ to leave a support structure which is at least partially formed of the collagen matrix of the organ.

84. A method as set forth in claim 66 wherein said step of positioning the support structure in the patient'"'"'s body includes connecting at least two portions of one or more blood vessels with the support structure.

85. A method as set forth in claim 84 wherein said step of connecting at least two portions of one or more blood vessels with the support structure includes connecting a portion of an artery with the support structure and connecting a portion of a vein with the support structure.

86. A method as set forth in claim 84 wherein said step of connecting at least two portions of one or more blood vessels with the support structure includes connecting a portion of one or more arteries with the support structure.

87. A method as set forth in claim 84 wherein said step of connecting at least two portions of one or more blood vessels with the support structure includes connecting a portion of one or more veins with the support structure.

88. A method as set forth in claim 66 wherein the modified biofilm is sterile.

89. A method as set forth in claim 66 wherein the polysaccharide-based modified biofilm is a glue-like substance.

90. A method as set forth in claim 66 wherein the polysaccharide-based modified biofilm provides initial attachment of viable cells to the support structure.

91. A method as set forth in claim 66 wherein attaching viable cells includes applying the polysaccharide-based modified biofilm to the support structure intracorporeally.

92. A method of implanting cells into a body of a patient, said method comprising the steps of removing a portion of an organ from a body, rendering the portion of an organ which is removed from the body non-antigenic to leave a support structure having a configuration corresponding to the configuration of the portion of an organ, preparing a polysaccharide-based modified biofilm from bacteria, attaching viable cells for implantation with the polysaccharide-based modified biofilm onto the support structure having a configuration corresponding to the configuration of the portion of an organ which is removed from a body, and positioning the support structure in the patient'"'"'s body with the viable cells disposed on the support structure.

93. A method of implanting cells into a body of a patient, said method comprising the steps of removing a portion of a blood vessel from the patient'"'"'s body, providing a tubular support structure, preparing a polysaccharide-based modified biofilm from bacteria, attaching viable cells for implantation with the polysaccharide-based modified biofilm onto the tubular support structure, connecting a first end portion of the tubular support structure with the blood vessel from which a portion was removed, and conducting a flow of blood between the tubular support structure and the blood vessel from which a portion was removed.

94. A method as set forth in claim 93 further including the step of connecting a second end portion of the tubular support structure with tissue in the patient'"'"'s body.

95. A method as set forth in claim 94 wherein said steps of connecting a first end portion of the tubular support structure with the blood vessel and connecting a second end portion of the tubular support structure with tissue includes positioning an expandable member with a first end portion of the expandable member enclosed by the blood vessel, a second end portion of the expandable member enclosed by the tissue, and a central portion of the expandable member enclosed by the tubular support structure, the central portion of the expandable member being disposed between the first and second end portions of the expandable member.

96. A method as set forth in claim 93 further including the step of connecting a second end portion of the tubular support structure with the blood vessel from which a portion was removed.

97. A method as set forth in claim 96 wherein said step of connecting a first end portion of the tubular support structure with the blood vessel and connecting a second end portion of the tubular support structure with the blood vessel includes positioning a balloon with a first end portion of the balloon enclosed by the blood vessel, a second end portion of the balloon enclosed by the blood vessel, and a central portion of the balloon enclosed by the tubular support structure, the central portion of the balloon being disposed between the first and second end portions of the balloon.

98. A method as set forth in claim 93 wherein said step of connecting a first end portion of the tubular support structure with the blood vessel includes positioning a balloon with a first portion of the balloon enclosed by the blood vessel and a second portion of the balloon enclosed by the tubular support structure.

99. A method as set forth in claim 93 wherein said step of connecting the first end portion of the tubular support structure with the blood vessel includes aligning the blood vessel with the first end portion of the tubular support structure by applying force against an inner surface area on the blood vessel.

100. A method as set forth in claim 99 wherein said step of applying force against an inner surface area on the blood vessel includes expanding an expandable member with at least a portion of the expandable member disposed in the blood vessel.

101. A method as set forth in claim 100 wherein said step of expanding an expandable member is performed with a portion of the expandable member in the tubular support structure.

102. A method as set forth in claim 93 wherein said step of providing a tubular support structure includes removing cells from the portion of the blood vessel removed from the patient'"'"'s body to leave a tubular support structure having a configuration corresponding to the configuration of the portion of the blood vessel removed from the patient'"'"'s body.

103. A method as set forth in claim 102 wherein said step of removing cells from the portion of the blood vessel removed from the patient'"'"'s body includes at least partially maintaining a collagen matrix of the portion of the blood vessel to leave a tubular support structure which is at least partially formed of the collagen matrix of the portion of the blood vessel removed from the patient'"'"'s body.

104. A method as set forth in claim 93 wherein said step of attaching viable cells on the tubular support structure includes attaching endothelial cells on the tubular support structure.

105. A method of implanting cells into a body of a patient, said method comprising the steps of:

providing a support structure containing a barrier being impervious to blood flow, the barrier being formed separately from the support structure and mounted in the support structure;

preparing a polysaccharide-based modified biofilm from bacteria

attaching viable cells for implantation onto the support structure adjacent the impervious barrier with the polysaccharide-based modified biofilm;

positioning the support structure in the patient'"'"'s body with the viable cells disposed on the support structure; and

directing a flow of blood throughout the support structure with the impervious barrier to supply said viable cells with the blood.

106. A method as set forth in claim 105 wherein said step of directing a flow of blood with the barrier includes conducting a flow of blood along opposite sides of the barrier.

107. A method as set forth in claim 105 further including the steps of connecting one portion of a blood vessel in the patient'"'"'s body with a first portion of the support structure and connecting another portion of a blood vessel in the patient'"'"'s body with a second portion of the support structure, the barrier being at least partially disposed between the first and second portions of the support structure.

108. A method as set forth in claim 105 wherein the barrier includes a plurality of walls disposed within the support structure and the flow of blood through the support structure is conducted along surfaces of the walls.

109. A method as set forth in claim 105 further including the steps of connecting a first portion of a blood vessel in the patient'"'"'s body with a first portion of the support structure, connecting a second portion of a blood vessel in the patient'"'"'s body with a second portion of the support structure, and connecting a third portion of a blood vessel in the patient'"'"'s body with a third portion of the support structure, said step of directing a flow of blood through the support structure includes directing a flow of blood between the first, second and third portions of the support structure.

110. A method as set forth in claim 105 wherein the barrier is connected with opposite sides of the support structure.

111. A method as set forth in claim 105 wherein the barrier is spaced from at least one side wall of the support structure.

112. A method as set forth in claim 105 wherein the barrier is integrally formed as one-piece with the structural support.

113. A method as set forth in claim 105 wherein the barrier includes a plurality of interconnected members.

114. A method as set forth in claim 105 wherein the barrier includes a plurality of bends forming a maze and wherein directing a flow of blood through the support structure includes directing a flow of blood through the maze.

115. A method as set forth in claim 105 wherein the barrier includes an arcuate configuration and wherein directing a flow of blood through the support structure includes directing a flow of blood with the arcuate configuration.

116. A method as set forth in claim 105 wherein the barrier includes a flow splitter and wherein directing a flow of blood through the support structure includes dividing a flow of flood with the flow splitter.

117. A method of implanting cells into a body of a patient, said method comprising the steps of:

shaping a support structure to have an external configuration corresponding to an external configuration of at least a portion of a selected organ, the support structure having a barrier disposed thereon, the barrier being impervious to blood flow;

preparing a polysaccharide-based modified biofilm from bacteria;

attaching viable cells for implantation with the polysaccharide-based modified biofilm onto the support structure, the viable cells positioned on opposite sides of the impervious barrier;

removing at least a portion of the selected organ from the patient'"'"'s body;

positioning the support structure in the patient'"'"'s body at the location where at least a portion of the selected organ was removed from the patient'"'"'s body; and

dispersing the blood flow throughout the support structure with the barrier.

118. A method as set forth in claim 117 wherein said step of positioning the support structure in the patient'"'"'s body includes connecting a plurality of blood vessels with the support structure.

119. A method as set forth in claim 117 wherein said step of shaping a support structure to have an external configuration corresponding to an external configuration of at least a portion of the selected organ includes providing a pattern having an external configuration of at least a portion of the selected organ, forming a mold around at least a portion of the pattern, separating the mold and pattern to leave a mold cavity, and shaping material in the mold cavity to form the support structure.

120. A method set forth in claim 119 wherein said step of shaping material in the mold cavity to form the support structure includes forming a porous support structure, said step of attaching viable cells on the support structure includes attaching viable cells in the support structure.

121. A method as set forth in claim 119 wherein said step of shaping material in the mold cavity to form the support structure includes intertwining one or more strands in the mold cavity to at least partially form the support structure.

122. A method as set forth in claim 121 wherein said step of attaching viable cells on the support structure includes attaching the viable cells in openings formed by intertwining one or more strands in the mold cavity.

123. A method as set forth in claim 122 wherein said step of attaching viable cells in openings formed by intertwining one or more strands in the mold cavity includes exposing the support structure to a liquid containing viable cells.

124. A method as set forth in claim 117 wherein said step of positioning viable cells on the support structure includes exposing the support structure to a liquid containing viable cells.

125. A method as set forth in claim 117 wherein the barrier is connected with opposite sides of the support structure.

126. A method as set forth in claim 117 wherein the barrier is spaced from side walls of the support structure.

127. A method as set forth in claim 117 wherein the barrier is integrally formed as one-piece with the structural support.

128. A method as set forth in claim 117 wherein the barrier includes a plurality of interconnected members.

129. A method as set forth in claim 117 wherein the barrier includes a plurality of bends forming a maze such that blood flow is directed through the maze.

130. A method as set forth in claim 117 wherein the barrier includes an arcuate configuration such that blood flow is directed by the arcuate configuration.

131. A method as set forth in claim 117 wherein the barrier includes a flow splitter such that blood flow is directed by the flow splitter.