BMP products

• US 7,300,772 B2
• Filed: 03/09/2001
• Issued: 11/27/2007
• Est. Priority Date: 07/01/1986
• Status: Expired due to Fees

• Current Assignee: Genetics Institute LLC (Pfizer Inc.)
• Original Assignee: Genetics Institute LLC (Pfizer Inc.)
• Inventors: Wozney, John M., Wang, Elizabeth A., Rosen, Vicki A.
• Primary Examiner(s): ROMEO, DAVID S
• Application Number:
  US09/804,625
• Publication Number:
  US 20030049826A1
• Time in Patent Office: 2,454 Days
• Field of Search: 435/69.1, 435/252.3, 435/325, 435/320.1, 536/23.5, 536/24.1, 530/300, 530/350
• US Class Current: 435/69.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61L 24/043 Mixtures of macromolecular materials
• CPC Class Codes: A61L 27/227 Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
• CPC Class Codes: C07K 14/51 Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00
• CPC Class Codes: Y10S 930/12 Growth hormone, growth factor other than t-cell or b-cell growth factor, and growth hormone releasing factor; related peptides

Abstract

Purified BMP-2 and BMP-4 proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and cartilage defects and in wound healing and related tissue repair.

Claims

1. An isolated polynucleotide comprising:

a) nucleotide 1252 through 1543 of SEQ ID NO;

3;

or

b) nucleotides encoding amino acids 299 through 396 of SEQ ID NO;

4.

4. A vector comprising a polynucleotide of claim 1 in operative association with an expression control sequence therefor.

5. A host cell transformed with a vector of claim 4.

6. A method for producing a bone morphogenetic protein, said method comprising the steps of:

a) culturing a host cell transformed with a polynucleotide of claim 1, and

b) recovering said bone morphogenetic protein.

2. An isolated polynucleotide comprising nucleotides 1252 through 1543 of SEQ ID NO:

3.

3. An isolated polynucleotide comprising nucleotides encoding amino acids 299 through 396 of SEQ ID NO:

4.