Apparatus and method for determining tissue characteristics

• US 7,301,629 B2
• Filed: 02/09/2006
• Issued: 11/27/2007
• Est. Priority Date: 05/19/1998
• Status: Expired due to Term

• Current Assignee: Spectro Incorporated (Spectro Scientific, Inc.)
• Original Assignee: Spectro Incorporated (Spectro Scientific, Inc.)
• Inventors: Arche, Glenn Steven, Faupel, Mark L., Bambot, Shabbir
• Primary Examiner(s): STAFIRA, MICHAEL PATRICK
• Application Number:
  US11/351,054
• Publication Number:
  US 20060126064A1
• Time in Patent Office: 656 Days
• Field of Search: None
• US Class Current: 356/337
• CPC Class Codes: A61B 5/0071 by measuring fluorescence emission
• CPC Class Codes: A61B 5/0084 for introduction into the body, e.g. by catheters A61B5/1459 takes precedence
• CPC Class Codes: G01N 2021/6484 Optical fibres
• CPC Class Codes: G01N 21/474 Details of optical heads therefor, e.g. using optical fibres
• CPC Class Codes: G01N 21/49 within a body or fluid
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence

Abstract

An apparatus and method embodying the invention include utilizing a device with a limited number of interrogation devices to accomplish a large number of measurements on a target tissue (50). An instrument embodying the invention includes a plurality of detection devices (54) that are arranged in a predetermined pattern on a tissue contacting face of the instrument. The face of the instrument is located adjacent the target tissue (50), and a plurality of tissue characteristic measurement are simultaneously conducted. The detection devices (54) are moved to a new position, preferably without moving the tissue contacting face, and a second plurality of tissue characteristic measurements are simultaneously conducted. By conducting a series of measurements cycles in this manner, the ultimate resolution of the device is increased, while still obtaining a given resolution, which reduces potential cross-talk errors. Further, a plurality of tissue characteristics are simultaneously obtained from locations spaced across the target tissue (50) during each measurement cycle.

Claims

1. An instrument for determining characteristics of a target material:

comprising;

an outer housing;

an inner core that is rotatably mounted within the outer housing;

a plurality of interrogation devices mounted on the inner core in a predetermined pattern; and

a detent mechanism attached to the inner core, wherein the detent mechanism is configured to allow the inner core to be rotated between a plurality of predetermined rotational positions relative to the outer housing; and

wherein stops are formed on an inner surface of the outer housing, and wherein the detent mechanism comprises at least one detent mount that is attached to the inner core and that is configured to interact with the stops to hold the inner core in the plurality of predetermined rotational positions.

2. The instrument of claim 1, wherein the plurality of interrogation devices are mounted on the inner core so that when the inner core is positioned at a first predetermined rotational position, the interrogation devices are positioned adjacent a first plurality interrogation positions relative to the outer housing, and wherein

rotation of the inner core from the first predetermined rotational position to a second predetermined rotational position causes the plurality of interrogation devices to be repositioned adjacent a second plurality of interrogation positions.

3. The instrument of claim 2, wherein the instrument is configured such that the plurality of interrogation devices are repositioned to a plurality of predetermined interrogation positions each time the inner core is rotated to a corresponding predetermined rotational position.

4. The instrument of claim 3, wherein none of the predetermined interrogation positions are coincident.

5. The instrument of claim 1, wherein the predetermined pattern in which the plurality of interrogation devices are mounted on the inner core minimizes cross-talk between adjacent interrogation devices.

6. The instrument of claim 1, wherein sensing portions of the plurality of interrogation devices are mounted on a face of the inner core, and wherein the predetermined pattern in which the plurality of interrogation devices are mounted on the inner core distributes the plurality of interrogation devices substantially evenly across the face of the inner core.

7. The instrument of claim 1, wherein the plurality of interrogation devices comprise a plurality of optical fibers.

8. The instrument of claim 7, wherein at least two optical fibers are located at each interrogation position, wherein at least one optical fiber at each interrogation position is configured to conduct excitation light to the interrogation position, and wherein at least one optical fiber at each interrogation position is configured to receive light that is scattered from or generated by a target material.

9. The instrument of claim 7, further comprising a detector array, wherein light scattered from or generated by a target material is conducted to the detector array by at least some of the optical fibers.

10. The instrument of claim 1, wherein each stop includes a depression, wherein each at least one detent mount includes a biased member, and wherein each biased member is configured to nest in a depression of a stop to hold the inner core in one of the plurality of predetermined rotational positions.

11. The instrument of claim 1, wherein the detent mechanism is configured to support at least a portion of the. inner core inside the outer housing.

12. The instrument of claim 1, wherein the outer housing includes an end cap, and wherein the plurality of interrogation devices are configured to project excitation light through the end cap and to detect light from a target material that passes through the end cap.

13. The instrument of claim 12, wherein an index matching agent is located between the end cap and the plurality of interrogation devices.

14. A method of detecting characteristics of a target material, comprising the steps of:

positioning a plurality of interrogation devices that are arranged in a pattern adjacent a first plurality of interrogation positions on a target material;

exciting at least a portion of the target material;

detecting characteristics of the target material at the first plurality of interrogation positions in response to excitation;

within a housing, sequentially positioning the capture of the plurality of interrogation devices so that they are adjacent at least one additional plurality of interrogation positions on the target material, wherein the first and at least one additional plurality of positions are not coincident; and

sequentially detecting characteristics of the target material at the at least one additional plurality of interrogation positions.

15. The method of claim 14, wherein each detecting step comprises the steps of:

detecting a first type of characteristics of the target material at a plurality of interrogation positions; and

detecting a second type of characteristics of the target material at a plurality of interrogation positions.

16. An instrument for determining characteristics of a target material, comprising:

an outer housing;

an excitation device capable of providing excitation energy to a target material;

an interrogation device capable of determining characteristics of a target material at a plurality of predetermined transmitting and interrogation locations arranged in a predetermined pattern; and

a positioner to adjust the detector for selectively reading tissue characteristics at different interrogation locations amongst a plurality of precisely predetermined positions relative to the outer housing;

a sequencer for activating the excitation and interrogation devices and said positioner so that the target material excited and interrogated in adjacent positions sequentially to determine characteristics of a target material at a plurality of adjacent interrogations locations, and wherein none of the interrogation locations are coincident but close enough, that if interrogated simultaneously, would not experience cross-talk between said locations.

17. A method of detecting characteristics of a target material, comprising the steps of:

positioning at least one excitation device and plurality of interrogation devices arranged in a pattern adjacent a first plurality of interrogation positions on a target material;

exciting the target;

detecting characteristics of the target material at the first plurality of interrogation positions;

sequentially re-aiming the plurality of interrogation devices within a housing so that they are adjacent at least one additional plurality of interrogation positions on the target material, wherein the first and at least one additional plurality of positions are not coincident but are sufficiently close that, if interrogated simultaneously would not experience cross-talk therebetween; and

detecting characteristics of the target material at the at least one additional plurality of interrogation positions.