Readable probe array for in-vivo use

• US 7,302,289 B2
• Filed: 06/14/2001
• Issued: 11/27/2007
• Est. Priority Date: 01/20/1998
• Status: Expired due to Fees

• Current Assignee: Boston Scientific Scimed, Inc. (Boston Scientific Corporation)
• Original Assignee: Scimed Life Systems Incorporated (Boston Scientific Corporation)
• Inventors: Crowley, Robert J.
• Primary Examiner(s): Casler; Brian L.
• Assistant Examiner(s): JUNG, WILLIAM C
• Application Number:
  US09/881,283
• Publication Number:
  US 20010029328A1
• Time in Patent Office: 2,357 Days
• Field of Search: 600/310, 600/317, 600/473, 600/476, 600/478, 600/326, 600/342, 600/433, 600/434, 600/435, 600/407, 600/423, 600/472, 604/21, 604/66, 604/507, 604/508, 604/503, 604/504, 604/506
• US Class Current: 600/478
• CPC Class Codes: A61B 5/0071 by measuring fluorescence emission
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/0084 for introduction into the body, e.g. by catheters A61B5/1459 takes precedence
• CPC Class Codes: A61B 5/0086 using infrared radiation
• CPC Class Codes: A61B 5/0097 by applying acoustic waves and detecting light, i.e. acoustooptic measurements
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates

Abstract

A disposable high density optically readable polydeoxynucleotide array with integral fluorescence excitation and fluorescence emission channels is described. The compact array size allows integration into several types of interventional devices such as catheters, guidewires, needles, trocars and may be used intraoperatively. Highly sensitive monitoring of the metabolic and disease pathways of cells in vivo under varying chemical, genetic and environmental conditions is afforded.

Claims

1. A body-insertable apparatus comprising:

an array of probes, each of the probes comprising at least one probe material having an affinity for one or more constituent molecules in a body;

a light source capable of generating light, wherein the generated light impinges upon at least one probe material and causes a secondary light to be emitted from the probe material when the probe material is linked to a molecule to which the probe material has an affinity;

a detector for detecting the secondary emission of light of at least one of the probe materials and for converting the secondary emission of light to electrical signals, the light source, the probes, and the detector adapted for placement together in an area of interest within a body.

2. The apparatus of claim 1 wherein said probe material binds to an oligonucleotide.

3. The apparatus of claim 1 wherein said probe material binds to a protein.

4. The apparatus of claim 1 wherein said probe material is fluorescently labeled.

5. The apparatus of claim 1 wherein the probes comprise a readable polydeoxynucleotide array.

6. The apparatus of claim 5 wherein the probes are positioned adjacent to said detector.

7. The apparatus of claim 1 wherein the probes are disposed in a plurality of chambers within a frame.

8. The apparatus of claim 7 wherein said frame comprises a molded material.

9. The apparatus of claim 7 wherein said frame comprises a foraminous material.

10. The apparatus of claim 1 further comprising optics that direct the light to at least one of the probes.

11. The apparatus of claim 10 wherein said optics comprises a mirror.

12. The apparatus of claim 11 wherein said mirror is adjustable.

13. The apparatus of claim 1 wherein said body-insertable apparatus is electrically connected to a processing unit.

14. The apparatus of claim 1 wherein said body-insertable apparatus is electrically connected to an amplifier.

15. The apparatus of claim 1 wherein said body-insertable apparatus is electrically connected to a display.

16. The apparatus of claim 1 wherein the light source comprises a light-emitting diode.

17. The apparatus of claim 1 wherein the light source is capable of generating light at wavelengths in a range from about 1100 nm to about 250 nm.

18. The apparatus of claim 1 wherein said detector comprises a photodiode responsive to light emitted by said probe material.

19. The apparatus of claim 1 wherein said detector comprises a light wavelength detection system.

20. The apparatus of claim 19 wherein said light wavelength detection system comprises a bandpass filter.

21. The apparatus of claim 1 wherein said body-insertable apparatus comprises a catheter.

22. The apparatus of claim 1 wherein said body-insertable apparatus defines one or more lumens extending through the length of said body-insertable apparatus.

23. The apparatus of claim 22 wherein said lumen delivers a drug, a reagent or a device to or beyond a distal tip of said body-insertable apparatus.

24. The apparatus of claim 22 wherein said lumen provides suction sufficient to draw an analyte into proximity with the light source, at least one of the probes and the detector such that the analyte can be analyzed.

25. The apparatus of claim 22 wherein said lumen comprises an infusion lumen.

26. The apparatus of claim 1 wherein said detector detects light emission at multiple wavelengths.

27. The apparatus of claim 26 wherein said detector comprises a photodiode.

28. A method of performing in vivo examination of a mammalian body, said method comprising:

(a) providing a device comprising a light source, an array of probes, and a detector wherein each of the probes comprises at least one probe material having an affinity for an analyte exposed to the analyte;

(b) inserting said device into said mammalian body until said probe contacts an analyte in an area of interest;

(c) generating light from the light source to illuminate at least one of the probes containing at least one probe material that emits a secondary light when the probe material is in contact with the analyte to which the probe material has an affinity;

(d) detecting the secondary light emitted by at least one of the probe materials through said detector; and

(e) converting said secondary light to an electrical signal using said detector.

29. The method of claim 28 comprising contacting at least one of the probes with an oligonucleotide.

30. The method of claim 28 comprising contacting at least one of the probes with a protein.

31. The method of claim 28 comprising providing at least one probe that is fluorescently labeled.

32. The method of claim 28 further comprising providing the probes with a readable polydeoxynucleotide array.

33. The method of claim 28 further comprising disposing the probes in a plurality of chambers within a frame.

34. The method of claim 33 further comprising providing said frame at least partly in a molded material.

35. The method of claim 33 further comprising providing said frame at least partly in a foraminous material.

36. The method of claim 28 further comprising using optics to direct the light generated from the light source to at least one of the probes.

37. The method of claim 36 wherein said step of using optics comprises adjusting a mirror.

38. The method of claim 28 further comprising transmitting and processing said electrical signal.

39. The method of claim 28 further comprising amplifying said electrical signal.

40. The method of claim 28 further comprising displaying said electrical signal.

41. The method of claim 28 further comprising mixing the probe material with an ink to form a probe-material-filled ink and depositing said probe-material-filled ink upon a substrate.

42. The method of claim 41 further comprising depositing a plurality of probe-material-filled inks upon said substrate in a specific ink pattern.

43. The method of claim 42 further comprising protecting said ink pattern with a topcoat.

44. The method of claim 43 wherein said topcoat comprises a dissolvable gel.

45. The method of claim 43 wherein said topcoat comprises a polymer material dissolvable only upon application of a solvent.

46. The method of claim 28 comprising providing the light source with a light-emitting diode.

47. The method of claim 28 wherein step (c) comprises generating radiation of wavelengths in a range from about 1100 nm to about 250 nm.

48. The method of claim 28 comprising providing said detector with a photodiode responsive to said secondary light emitted from the at least one of the probe materials.

49. The method of claim 28 comprising providing said detector with a light wavelength detection system.

50. The method of claim 49 further comprising providing said light wavelength detection system with a bandpass filter.

51. The method of claim 28 comprising providing said device with a catheter.

52. The method of claim 28 comprising providing said device with at least one lumen extending through the length of said device.

53. The method of claim 52 further comprising delivering a drug, a reagent or a device through said lumen to or beyond a distal tip of said device to affect said area of interest.

54. The method of claim 52 further comprising using said lumen to provide suction such that said analyte is drawn into contact with at least one of the probes.

55. The method of claim 28 further comprising implanting said device in said mammalian body.

56. The method of claim 28 further comprising using a carrying device to deliver said device to the area of interest.

57. The method of claim 56 further comprising selecting said carrying device from the group consisting of a hollow needle, a guide wire, a balloon catheter, an ultrasound catheter, an introducer sheath, and a balloon angioplasty catheter.

58. A method of performing in vivo examination of a mammalian body, said method comprising:

(a) providing a device comprising a light source, an array of probes, and a detector wherein each of the probes comprises at least one optically detectable probe material having an affinity for an analyte and an optically detectable property when the probe material is exposed to the analyte;

(b) inserting said device into said mammalian body until said probe contacts an analyte in an area of interest;

(c) generating light from the light source to illuminate at least one of the probes;

(d) detecting an optical signal representative of the optically detectable property of at least one of the probes through said detector;

(e) converting said optical signal to an electrical signal using said detector; and

(f) introducing to said area of interest a lysing system to facilitate contact between said analyte and at least one of the probes.

59. The method of claim 58 further comprising using ultrasonic energy to rupture a cell membrane at said area of interest.

60. The method of claim 58 further comprising using a pressurization and evacuation system to rupture a cell membrane at said area of interest.

61. The method of claim 58 further comprising using a mechanical force to rupture a cell membrane at said area of interest.

62. The method of claim 58 further comprising using a lysing head driven by a driveshaft to rupture said cell membrane.

63. A method of performing in vivo examination of a mammalian body, said method comprising:

(a) providing a device comprising a light source, an array of probes, and a detector wherein each of the probes comprises at least one optically detectable probe material having an affinity for an analyte and an optically detectable property when the probe material is exposed to the analyte;

(b) inserting said device into said mammalian body until said probe contacts an analyte in an area of interest;

(c) generating light from the light source to illuminate at least one of the probes;

(d) detecting an optical signal representative of the optically detectable property of at least one of the probes through said detector;

(e) converting said optical signal to an electrical signal using said detector; and

(f) anchoring said device in said area of interest through an anchor.

64. The method of claim 63 further comprising providing said anchor with a therapeutic tip for administering a therapeutic agent.

65. The method of claim 64 further comprising separating said therapeutic tip from the rest of said device and leaving said therapeutic tip within the area of interest after removal of said device.

66. The method of claim 64 further comprising retrieving said therapeutic tip.

67. The method of claim 66 further comprising retrieving said therapeutic tip through a tether attached to said therapeutic tip.

68. The method of claim 64 further comprising controlling a function of said therapeutic tip from outside said body by transmitting an electrical signal through a tether attached to said therapeutic tip.

69. A method of performing in vivo examination of a mammalian body, said method comprising:

(a) providing a device comprising a light source, an array of probes, and a detector, wherein each of the probes comprises at least one optically detectable probe material;

(b) inserting said device into said mammalian body until said probe contacts an analyte in an area of interest;

(c) introducing to said area of interest a lysing system to facilitate contact between said analyte and at least one of the probes;

(d) generating light from the light source to illuminate at least one of the probes;

(e) detecting an optical signal representative of an optical property of at least one of the probes through said detector; and

(f) converting said optical signal to an electrical signal using said detector.

70. The method of claim 69 further comprising using ultrasonic energy to rupture a cell membrane at said area of interest.

71. The method of claim 69 further comprising using a pressurization and evacuation system to rupture a cell membrane at said area of interest.

72. The method of claim 69 further comprising using a mechanical force to rupture a cell membrane at an area of interest.

73. The method of claim 72 further comprising using a lysing head driven by a drive shaft to rupture said cell membrane.

74. A method for performing in vivo examination of a mammalian body, the method comprising:

(a) providing a device comprising a light source, an array of probes, and a detector, wherein each of the probes comprises at least one optically detectable probe material;

(b) inserting said device into a mammalian body until said probe contacts an analyte in an area of interest;

(c) anchoring said device in said area of interest with an anchor;

(d) generating light from the light source to illuminate at least one of the probes;

(e) detecting an optical signal representative of an optical property of at least one of the probes through said detector; and

(f) converting said optical signal to an electrical signal using said detector.

75. The method of claim 74 further comprising providing said anchor with a therapeutic tip for administering a therapeutic agent.

76. The method of claim 75 further comprising separating said therapeutic tip from the rest of said device and leaving said therapeutic tip within the area of interest after removal of said device.

77. The method of claim 75 further comprising retrieving said therapeutic tip.

78. The method of claim 77 further comprising retrieving said therapeutic tip through a tether attached to said therapeutic tip.

79. The method of claim 75 further comprising controlling a function of said therapeutic tip from outside the body by transmitting an electrical signal through a tether attached to said therapeutic tip.