Hookworm vaccine

• US 7,303,752 B2
• Filed: 04/16/2004
• Issued: 12/04/2007
• Est. Priority Date: 10/17/2001
• Status: Expired due to Fees

• Current Assignee: George Washington University
• Original Assignee: George Washington University
• Inventors: Goud, Gaddam, Bottazzi, Maria Elena, Bethony, Jeffrey, Zhan, Bin, Wang, Yan, Jones, Brian, Loukas, Alexander, Hotez, Peter, Bdamchian, Mahnaz, Mendez, Susana, Hawdon, John, Williamson, Angela, Ashcom, James
• Primary Examiner(s): Zeman; Robert A.
• Application Number:
  US10/825,692
• Publication Number:
  US 20050042232A1
• Time in Patent Office: 1,327 Days
• Field of Search: 424/184.1, 424/185.1, 424/191.1, 530/350
• US Class Current: 424/191.1
• CPC Class Codes: A61K 2039/55577 Saponins; Quil A; QS21; ISCOMS
• CPC Class Codes: A61K 39/0003 Invertebrate antigens
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

Preparations which elicit an immune response to hookworm antigens and which may be utilized as hookworm vaccines are provided. In addition, a method of increasing the effectiveness of vaccinations against infectious diseases in patients infected with hookworm is provided. The method involves chemically treating the hookworm infestation prior to administering the vaccine.

Claims

1. A method of reducing the worm burden of human hookworm Necator americanus in a mammal or eliciting an immune response in said mammal comprising the step of:

administering to said mammal an effective amount of a composition comprising Na-ASP-2 having the amino acid sequence of SEQ ID NO;

69;

an adult stage hookworm antigen selected from the group consisting of Na-APR-1 having the amino acid sequence of SEQ ID NO;

8 and Na-CP-2 having the amino acid sequence of SEQ ID NO;

84; and

at least one adjuvant, wherein the immune response is directed against the Necator americanus antigens of the administered composition.

2. The method of claim 1, wherein said adult stage hookworm antigen is Na-APR-1 having the amino acid of SEQ ID NO:

8.

3. The method of claim 1, wherein said at least one adjuvant comprises two different adjuvants.

4. The method of claim 3, wherein one of said two different adjuvants is an aluminum hydroxide gel.