Choline transport like (CTL) membrane proteins involved in choline transport

• US 7,303,895 B1
• Filed: 11/03/2000
• Issued: 12/04/2007
• Est. Priority Date: 11/05/1999
• Status: Expired due to Fees

• Current Assignee: Centre National De La Recherche Scientifique
• Original Assignee: Centre National De La Recherche Scientifique
• Inventors: Traiffort, Elisabeth, Ruat, Martial, O'Regan, Seana, Meunier, François
• Primary Examiner(s): Chernyshev; Olga N.
• Application Number:
  US10/129,440
• Time in Patent Office: 2,587 Days
• Field of Search: 435/69.1, 435/320.1, 435/7.1, 435/6, 536/23.1, 536/23.5, 530/350
• US Class Current: 435/69.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61P 1/00 Drugs for disorders of the alimentary tract or the digestive system
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 25/02 for peripheral neuropathies
• CPC Class Codes: A61P 25/14 for treating abnormal movements, e.g. chorea, dyskinesia
• CPC Class Codes: A61P 25/16 Anti-Parkinson drugs
• CPC Class Codes: A61P 25/22 Anxiolytics
• CPC Class Codes: A61P 25/28 for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C12N 2799/021 for the expression of a heterologous nucleic acid

Abstract

The invention concerns the identification of a novel family of CTL (Choline Transporter Like) genes, in particular hCTL1 and hCTL2, involved in the metabolism and/or transport of choline in cells such as the intestinal tract cells, nervous cells, in particular motoneurons, sensitive neurons, neurons of the nucleus dorsalis of the spinal cord and oligodendrocytes. The invention opens up new prospects in particular for the treatment of familial dysautonomia, and Tangier disease. More generally, the identification of CTL genes enables to develop new strategies for treating diseases of the nervous system, in particular neurodegenerative demyelenating diseases, particularly Alzheimer disease, Parkinson disease and Huntington disease.

Claims

1. A purified or isolated nucleic acid comprising a nucleic acid sequence selected from the group consisting of:

a) the sequence SEQ ID NO;

1;

b) the complementary sequence of SEQ ID NO;

1; and

c) the RNA sequence corresponding to SEQ ID NO;

1.

2. An isolated nucleic acid encoding a polypeptide selected from the group consisting of:

SEQ ID NO;

3; and

SEQ ID NO;

9.

3. A vector comprising a nucleic acid sequence selected from the group consisting of:

a) the sequence SEQ ID NO;

1; and

b) the complementary sequence or the RNA sequence corresponding to SEQ ID NO;

1.

5. The vector of claim 3 or 4, wherein the sequence is fused with a promoter which is effective in eukaryotic or prokaryotic cells.

6. An isolated cell transformed with the vector of claim 3 or 4.

4. A vector comprising a nucleic acid sequence encoding a polypeptide selected from the group consisting of:

SEQ ID NO;

3; and

SEQ ID NO;

9.

7. A method for the amplification and/or detection of a target nucleic acid in a sample, said target nucleic acid having a sequence selected from the group consisting of:

a) the sequence SEQ ID NO;

1;

b) the complementary sequence or the RNA sequence corresponding to a sequence as defined in a); and

c) a sequence capable of hybridizing to a sequence as defined in a) or b), the method comprising;

hybridizing the sample with a primer and/or probe comprising at least 12 contiguous nucleotides comprising a sequence complementary to said target polynucleotide in the sample, and which probe specifically hybridizes to said target polynucleotide, under conditions whereby a hybridization complex is formed between said probe and said target nucleic acid.

8. A method for the amplification and/or detection of a target nucleic acid in a sample, said target nucleic acid encoding a polypeptide selected from the group consisting of:

a) SEQ ID NO;

3; and

b) SEQ ID NO;

9;

or a sequence capable of hybridizing to a nucleic acid sequence encoding a) or b) the method comprising;

hybridizing the sample with a primer and/or probe comprising at least 12 contiguous nucleotides comprising a sequence complementary to said target polynucleotide in the sample, and which probe specifically hybridizes to said target polynucleotide, under conditions whereby a hybridization complex is formed between said probe and said target nucleic acid.