Endospore detector

• US 7,306,942 B1
• Filed: 05/28/2004
• Issued: 12/11/2007
• Est. Priority Date: 11/19/2001
• Status: Expired due to Fees

• Current Assignee: Antony R. Shoaf
• Original Assignee: Antony R. Shoaf
• Inventors: Shoaf, Antony R.
• Primary Examiner(s): REDDING, DAVID A
• Application Number:
  US10/857,760
• Time in Patent Office: 1,292 Days
• Field of Search: None
• US Class Current: 435/288.7
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH
• CPC Class Codes: Y10T 436/19 Halogen containing
• CPC Class Codes: Y10T 436/193333 In aqueous solution
• CPC Class Codes: Y10T 436/196666 Carbon containing compound [e.g., vinylchloride, etc.]

Abstract

The invention herein provides for the detection of certain calcium containing endospores, and particularly pertains to the detection of bacillus anthracis by first chelating calcium ions of said endospores and then reacting the chelated calcium ions with aequorin to generate a light pulse which can then be detected by a standard liquid scintillation spectrometer.

Claims

1. A product for detecting calcium containing endospores comprising:

a reaction vessel, an air pump, an air intake conduit, said air pump communicating with said air intake conduit, a chemiluminescent liquid, said chemiluminescent liquid contained within said reaction vessel, said air intake conduit submerged within said chemiluminescent liquid, said chemiluminescent liquid comprising aequorin and a calcium chelating agent, a photon counter, said photon counter comprising a photomultiplier and a ratemeter, said photon counter positioned proximate said reaction vessel for sensing photons of light immediately upon occurrence in said reaction vessel, a computer, said photon counter connected to said computer, whereby calcium containing endospores directed into said reaction vessel will immediately react with said chemiluminescent liquid emitting photons of light for detection by said photon counter and reading by said computer.

2. The product of claim 1 wherein said photomultiplier contacts said reaction vessel.

3. The product of claim 1 wherein said calcium chelating agent comprises ethylenediamine tetraacetate, tetrasodium salt.

4. The product of claim 1 wherein said aequorin is extracted from jellyfish.

5. The product of claim 1 wherein said chemiluminescent liquid comprises aequorin dissolved in an ethylenediamine tetraacetate buffer solution.

6. The product of claim 1 wherein said photon counter comprises a liquid scintillator spectrometer.

7. The product of claim 1 wherein said photon counter comprises a pair of photomultipliers.

8. The product of claim 1 wherein said air intake conduit has a diameter of 4 mm.

9. The product of claim 1 wherein said air pump forces air through said air intake conduit at approximately 12.5 liters per minute.

13. The product of claim 1 further comprising an air tube, a particulate filter, said particulate filter contained within said air tube and said air tube communicating with said air pump.

10. A product for detecting calcium containing endospores comprising:

a reaction vessel, an intake conduit, said intake conduit in fluid communication with said reaction vessel, a chemiluminescent liquid, said chemiluminescent liquid contained within said reaction vessel, an air pump, said air pump mounted proximate said intake conduit, said conduit submerged within said chemiluminescent liquid, a liquid scintillation spectrometer, said liquid scintillation spectrometer proximate said reaction vessel for immediately detecting photons of light as they occur in said reaction vessel, said scintillation spectrometer comprising a pair of photomultipliers, a ratemeter, said photomultipliers in electrical communication with said ratemeter, a chart recorder, said chart recorder in electrical communication with said ratemeter, a printer, said printer in electrical communication with said chart recorder, said chemiluminescent liquid comprising aequorin, a chelating agent, said chelating agent mixed with said aequorin, whereby endospores containing calcium forced by said air pump into said chemiluminescent liquid will immediately react with said aequorin to generate light pulses for detection by said liquid scintillation spectrometer.

11. The product of claim 10 wherein said chelating agent comprises ethylenediamine tetraacetate, tetrasodium salt.

12. A product for detecting calcium containing endospores comprising:

a reaction vessel, a chemiluminescent liquid, said chemiluminescent liquid contained within said reaction vessel, an air intake conduit, said air intake conduit comprising a terminal end, said terminal end submerged within said chemiluminescent liquid, said chemiluminescent liquid comprising aequorin and a calcium chelating agent, a photon counter, said photon counter positioned proximate said reaction vessel to sense photons of light immediately upon occurrence in said reaction vessel, whereby calcium containing endospores directed through said air intake conduit and into said chemiluminescent liquid will immediately react with said chemiluminescent liquid emitting photons of light for detection.

14. The product of claim 12 further comprising an air tube, a particulate filter, said particulate filter contained within said air tube and said air tube communicating with said air pump.