Detection of disease by analysis of emissions

• US 7,306,953 B2
• Filed: 01/14/2005
• Issued: 12/11/2007
• Est. Priority Date: 07/18/2002
• Status: Expired due to Term

• Current Assignee: University of The West of England Bristol
• Original Assignee: University of The West of England Bristol
• Inventors: Ratcliffe, Norman Mark, Probert, Christopher Simon J.
• Primary Examiner(s): WALLENHORST, MAUREEN
• Application Number:
  US11/036,206
• Publication Number:
  US 20060008918A1
• Time in Patent Office: 1,061 Days
• Field of Search: 436/63, 436/181, 436/173, 436/161, 436/106
• US Class Current: 436/181
• CPC Class Codes: G01N 33/497 of gaseous biological material, e.g. breath
• CPC Class Codes: Y10T 436/17 Nitrogen containing
• CPC Class Codes: Y10T 436/24 Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
• CPC Class Codes: Y10T 436/25875 Gaseous sample or with change of physical state

Abstract

A method of determining the cause of disease is described, which method uses the detection of “signature” or “fingerprint” volatile compounds in an emission, especially flatus, from a patient.

Claims

1. A method of analysing fluid emissions from a body to diagnose a disease state in said body, the method comprising the steps of:

collecting a sample of fluid emission from a body, and

analysing the emission solely for the presence or absence of at least one predetermined volatile compound wherein the emission is flatus or gas evolved from stool, wherein the presence or absence of said predetermined volatile compound in said emission is indicative of a disease state, to determine the presence or absence of said disease state in said body.

2. A method according to claim 1, wherein the disease is a disease of the gastrointestinal tract.

3. A method according to claim 1, wherein the emission is collected non-invasively.

4. A method according to claim 3, wherein the emission is collected by use of a solid sorbent.

5. A method according to claim 4, wherein the solid sorbent is a polydimethylsiloxane-coated fibre, Tenax, or a smart membrane.

6. A method according to claim 5, wherein the emission is analysed at least one of mass spectroscopy, gas chromatography and electronic nose.

7. A method according to claim 5, wherein the emission is de-sorbed prior to analysis.

8. A method according to claim 5, wherein the predetermined volatile compound is selected from the group consisting of furans, ammonia, amines, acids, carboxylic acids, esters, ethers, alcohols, sulfides, sulphates, thiols, aldehydes, ketones, alkanes, alkenes, terpenes, phenols, indoles, nitrates, aromatic compounds, hydrocarbons and sugars.

9. A method according to claim 4, wherein the emission is analysed at least one of mass spectroscopy, gas chromatography and electronic nose.

10. A method according to claim 4, wherein the emission is de-sorbed prior to analysis.

11. A method according to claim 4, wherein the predetermined volatile compound is selected from the group consisting of furans, ammonia, amines, acids, carboxylic acids, esters, ethers, alcohols, sulfides, sulphates, thiols, aldehydes, ketones, alkanes, alkenes, terpenes, phenols, indoles, nitrates, aromatic compounds, hydrocarbons and sugars.

12. A method according to claim 3, wherein the emission is collected by use of a liquid sorbent.

13. A method according to claim 12, wherein the emission is analysed by at least one of mass spectroscopy, gas chromatography and electronic nose.

14. A method according to claim 12, wherein the emission is de-sorbed prior to analysis.

15. A method according to claim 12, wherein the predetermined volatile compound is selected from the group consisting of furans, ammonia, amines, acids, carboxylic acids, esters, ethers, alcohols, sulfides, sulphates, thiols, aldehydes, ketones, alkanes, alkenes, terpenes, phenols, indoles, nitrates, aromatic compounds, hydrocarbons and sugars.

16. A method according to claim 3, wherein the emission is analysed by at least one of mass spectroscopy, gas chromatography and electronic nose.

17. A method according to claim 3, wherein the predetermined volatile compound is selected from the group consisting of furans, ammonia, amines, acids, carboxylic acids, esters, ethers, alcohols, sulfides, sulphates, thiols, aldehydes, ketones, alkanes, alkenes, terpenes, phenols, indoles, nitrates, aromatic compounds, hydrocarbons and sugars.

18. A method according to claim 1, wherein the emission is analysed by at least one of mass spectroscopy, gas chromatography and an electronic nose.

19. A method according to claim 18, wherein the predetermined volatile compound is selected from the group consisting of furans, ammonia, amines, acids, carboxylic acids, esters, ethers, alcohols, sulfides, sulphates, thiols, aldehydes, ketones, alkanes, alkenes, terpenes, phenols, indoles, nitrates, aromatic compounds, hydrocarbons and sugars.

20. A method according to claim 1, wherein the emission is de-sorbed prior to analysis.

21. A method according to claim 20, wherein the predetermined volatile compound is selected from the group consisting of furans, ammonia, amines, acids, carboxylic acids, esters, ethers, alcohols, sulfides, sulphates, thiols, aldehydes, ketones, alkanes, alkenes, terpenes, phenols, indoles, nitrates, aromatic compounds, hydrocarbons and sugars.

22. A method according to claim 1, wherein the predetermined volatile compound is selected from the group consisting of furans, ammonia, amines, acids, carboxylic acids, esters, ethers, alcohols, sulfides, sulphates, thiols, aldehydes, ketones, alkanes, alkenes, terpenes, phenols, indoles, nitrates, aromatic compounds, hydrocarbons and sugars.

23. A method according to claim 1, wherein said analysis is performed by use of a single sensor.

24. The method according to claim 1, wherein said analyzing of said emission for the presence or absence of at least one predetermined volatile compound comprises obtaining a signature pattern of said predetermined volatile compound, wherein the signature pattern of said predetermined volatile compound is indicative of a disease state.

25. A method of diagnosing the presence of a causative agent of a particular disease state in a patient suffering from diarrhoea, the method comprising the steps of:

collecting a sample of an emission from the patient, wherein the emission is flatus or gas evolved from stool,

analysing the emission for the presence or absence of at least one predetermined volatile compound to obtain a result for said predetermined volatile compound that is indicative of the causative agent of a particular disease state, and

diagnosing the presence of said causative agent from said result.

26. A method according to claim 25, wherein the disease is acute.

27. A method according to claim 25, wherein the disease is an infection.

28. A method according to claim 27, wherein the infection is selected from the group consisting of Clostridium difficle, rotavirus, Campylobacter spp., adenovirus, astrovirus, and small round-structured virus.

29. A method according to claim 25, wherein the disease results from parasitic infestation.

30. A method according to claim 29, wherein the parasitic infestation is selected from the group consisting of infestation with Giarda lamblia, Cryptospiridium spp., Leishmania spp., Schistoma spp., Onchocerca and lola (filariasis).

31. A method according to claim 25, wherein the disease is chronic.

32. A method according to claim 31, wherein the disease is selected from the group consisting of Crohn'"'"'s disease, Coeliac disease, ulcerative colitis, irritable bowel syndrome, inflammatory bowel disease, chronic pancreatitis, malabsorption syndrome, gastroenteritis, food intolerance and cancer.

33. A method according to claim 25, wherein the emission is collected non-invasively.

34. A method according to claim 25, wherein the emission is analysed by at least one of mass spectroscopy, gas chromatography electronic nose.

35. A method according to claim 25 wherein the predetermined volatile compound is selected from the group consisting of furans, ammonia, amines, acids, carboxylic acids, esters, ethers, alcohols, sulfides, sulphates, thiols, aldehydes, ketones, alkanes, alkenes, terpenes, phenols, indoles, nitrates, aromatic compounds, hydrocarbons and sugars.

36. A method according to claim 25, wherein said analysis is performed by use of a single sensor.

37. A method of analysing fluid emissions from a body to diagnose a disease state in said body, the method comprising the steps of:

collecting a sample of fluid emission from a body wherein the emission is flatus or gas evolved from a stool, and determining the absence of a predetermined volatile compound in said emission, wherein the absence of said predetermined volatile compound is indicative of a disease states

to diagnose said disease state in said body.

38. A method of diagnosing the presence of a causative agent of a particular disease state in a patient suffering from diarrhoea, the method comprising the steps of:

collecting a sample of an emission from the patient, wherein the emission is flatus or gas evolved from stool,

analysing the emission for the absence of at least one predetermined volatile compound to obtain a result for said predetermined volatile compound that is indicative of the causative agent of a particular disease state, and

diagnosing the presence of said causative agent from said result.