Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
First Claim
1. A complex comprising:
- a probe containing a heteropolymeric probe sequence of nucleic acids or nucleic acid analogues containing purines and pyrimidines; and
a target containing a heteropolymeric target sequence of nucleic acids or nucleic acid analogues containing purines and pyrimidines, wherein;
(a) at least one of said probe and said target is purified or synthetic;
(b) bonding of said heteropolymeric probe sequence to said heteropolymeric target sequence consists of;
(i) Watson-Crick bonds between each pyrimidine of the heteropolymeric probe sequence and each opposing purine of the heteropolymeric target sequence, and between each purine of the heteropolymeric probe sequence and each opposing pyrimidine of the heteropolymeric target sequence;
or(ii) homologous bonds between each pyrimidine of the heteropolymeric probe sequence and each opposing pyrimidine of the heteropolymeric target sequence, and between each purine of the heteropolymeric probe sequence and each opposing purine of the heteropolymeric target sequence;
and(c) all bases of said probe and said target are selected from the group consisting of A, T, G, C and U, provided that when said complex is a duplex and said heteropolymeric probe sequence is antiparallel to said heteropolymeric target sequence, said heteropolymeric probe sequence is bonded to said heteropolymeric target sequence by homologous base interaction, and provided that when said complex is a triplex, said complex is free of recombination proteins.
2 Assignments
0 Petitions
Accused Products
Abstract
A complex includes: (1) a probe containing a heteropolymeric probe sequence of nucleic acids or nucleic acid analogues; and (2) a target containing a heteropolymeric target sequence of nucleic acids or nucleic acid analogues, wherein: (a) at least one of the probe and the target is purified or synthetic; and (b) the heteropolymeric probe sequence is bonded to the heteropolymeric target sequence by Watson-Crick complementary base interaction or by homologous base interaction, provided that when the complex is a duplex and the heteropolymeric probe sequence is antiparallel to the heteropolymeric target sequence, the heteropolymeric probe sequence is bonded to the heteropolymeric target sequence by homologous base interaction, and provided that when the complex is a triplex, the complex is free of recombination proteins. A method for assaying a target includes detecting formation of the complex.
-
Citations
47 Claims
-
1. A complex comprising:
-
a probe containing a heteropolymeric probe sequence of nucleic acids or nucleic acid analogues containing purines and pyrimidines; and a target containing a heteropolymeric target sequence of nucleic acids or nucleic acid analogues containing purines and pyrimidines, wherein; (a) at least one of said probe and said target is purified or synthetic; (b) bonding of said heteropolymeric probe sequence to said heteropolymeric target sequence consists of; (i) Watson-Crick bonds between each pyrimidine of the heteropolymeric probe sequence and each opposing purine of the heteropolymeric target sequence, and between each purine of the heteropolymeric probe sequence and each opposing pyrimidine of the heteropolymeric target sequence;
or(ii) homologous bonds between each pyrimidine of the heteropolymeric probe sequence and each opposing pyrimidine of the heteropolymeric target sequence, and between each purine of the heteropolymeric probe sequence and each opposing purine of the heteropolymeric target sequence; and (c) all bases of said probe and said target are selected from the group consisting of A, T, G, C and U, provided that when said complex is a duplex and said heteropolymeric probe sequence is antiparallel to said heteropolymeric target sequence, said heteropolymeric probe sequence is bonded to said heteropolymeric target sequence by homologous base interaction, and provided that when said complex is a triplex, said complex is free of recombination proteins. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 19, 20, 21, 23, 24, 25, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42)
-
-
2. A complex comprising:
-
a probe containing a heteropolymeric probe sequence of nucleic acids or nucleic acid analogues; and a target containing a heteropolymeric target sequence of nucleic acids or nucleic acid analogues, wherein;
(a) at least one of said probe and said target is purified or synthetic;
(b) said complex is a duplex;
(c) said heteropolymeric probe sequence and said heteropolymeric target sequence have parallel directionality; and
(d) said heteropolymeric probe sequence is bound to said heteropolymeric target sequence by Watson-Crick complementary base interaction.
-
-
11. A complex comprising:
-
a probe containing a heteropolymeric probe sequence of nucleic acids or nucleic acid analogues; and a target containing a heteropolymeric target sequence of nucleic acids or nucleic acid analogues, wherein;
(a) at least one of said probe and said target is purified or synthetic;
(b) said heteropolymeric probe sequence is bonded to said heteropolymeric target sequence by Watson-Crick complementary base interaction or by homologous base interaction; and
(c) said probe and said target are double stranded and said complex is a quadruplex. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 22, 44, 46)
-
-
26. A complex comprising:
-
a probe containing a heteropolymeric probe sequence of nucleic acids or nucleic acid analogues; and a target containing a heteropolymeric target sequence of nucleic acids or nucleic acid analogues. wherein;
(a) at least one of said probe and said target is purified or synthetic;
(b) said heteropolymeric probe sequence is bonded to said heteropolymeric target sequence by base interaction between bases of said probe and bases of said target, said base interaction being Watson-Crick complementary base interaction or homologous base interaction;
(c) the probe further contains a loop and a stem adjacent to the loop;
(d) the heteropolymeric probe sequence is located partially or entirely within the stem; and
(e) each of the loop and the stem comprises nucleic acids or nucleic acid analogues, provided that when said complex is a duplex and said heteropolymeric probe sequence is antiparallel to said heteropolymeric target sequence, said heteropolymeric probe sequence is bonded to said heteropolymeric target sequence by homologous base interaction, and provided that when said complex is a triplex, said complex is free of recombination proteins. - View Dependent Claims (47)
-
-
43. An isolated or synthetic probe comprising:
-
a loop containing nucleic acids or nucleic acid analogues; a stem adjacent to the loop, and containing at least two strands of nucleic acids or nucleic acid analogues; and a heteropolymeric probe sequence located entirely or partially within the loop, and containing a double or triple-stranded sequence of nucleic acids or nucleic acid analogues, wherein the at least two strands of the stem are adapted to be bound to each other in the absence of the heteropolymeric target sequence binding to the heteropolymeric probe sequence, and the at least two strands are adapted to separate from each other and open the loop when a heteropolymeric target sequence binds to the heteropolymeric probe sequence.
-
-
45. A complex comprising:
-
a probe containing a heteropolymeric probe sequence of nucleic acids or nucleic acid analogues; and a target containing a heteropolymeric target sequence of nucleic acids or nucleic acid analogues, wherein; (a) at least one of said probe and said target is purified or synthetic; (b) bonding of said heteropolymeric probe sequence to said heteropolymeric target sequence consists of; (i) Watson-Crick bonds comprising at least two members selected from the group consisting of an A-T bond, an A-U bond, and a G-C bond, or (ii) homologous bonds comprising at least four members selected from the group consisting of an A-A bond, a T-T bond, a G-G bond, a C-C bond and a U-U bond; and (c) all bases of said probe and said target are selected from the group consisting of A, T, G, C and U, provided that when said complex is a duplex and said heteropolymeric probe sequence is antiparallel to said heteropolymeric target sequence, said heteropolymeric probe sequence is bonded to said heteropolymeric target sequence by homologous base interaction, and provided that when said complex is a triplex, said complex is free of recombination proteins.
-
Specification