Mass spectrometer

• US 7,309,861 B2
• Filed: 09/03/2003
• Issued: 12/18/2007
• Est. Priority Date: 09/03/2002
• Status: Active Grant

• Current Assignee: Micromass UK Ltd (Waters Corporation)
• Original Assignee: Micromass UK Ltd (Waters Corporation)
• Inventors: Bateman, Robert Harold, Brown, Jeffery Mark
• Primary Examiner(s): Wells; Nikita
• Application Number:
  US10/513,378
• Publication Number:
  US 20060076484A1
• Time in Patent Office: 1,567 Days
• Field of Search: None
• US Class Current: 250/290
• CPC Class Codes: H01J 49/062 Ion guides linear ion traps performing mass selection H01J49/4225, mass filters H01J49/421

Abstract

A mass spectrometer is disclosed comprising a guide wire ion guide 1 having an outer cylindrical electrode 2 and an inner guide wire electrode 3. AC and DC potential differences are maintained between the outer electrode 2 and the inner electrode 3 so that ions are radially confined within the ion guide 1 in an annular potential well. The outer electrode 2 may be segmented and axial potential wells created along the length of the ion guide 1 may be translated along the length of the ion guide 1 by applying additional transient DC potentials to the segments forming the outer electrode 2.

Claims

1. A mass spectrometer comprising an ion guide, said ion guide comprising an outer electrode and an inner electrode disposed within said outer electrode, wherein in use said inner and outer electrodes are maintained at a DC potential difference such that ions experience a first radial force towards said inner electrode and wherein in use an AC or RF voltage is applied to said inner and/or said outer electrodes so that ions experience a second radial force towards said outer electrode.

2. A mass spectrometer as claimed in claim 1, wherein said AC or RF voltage is a single phase AC or RF voltage applied to said inner electrode.

3. A mass spectrometer as claimed in claim 1, wherein said AC or RF voltage is a single phase AC or RF voltage applied to said outer electrode.

4. A mass spectrometer as claimed in claim 1, wherein said AC or RF voltage is a two phase AC or RF voltage and wherein a first phase is applied to said inner electrode and a second phase is applied to said outer electrode.

5. A mass spectrometer as claimed in claim 1, wherein said AC or RF voltage has a frequency selected from the group consisting of:

(i)<

100 kHz;

(ii) 100-200 kHz;

(iii) 200-300 kHz;

(iv) 300-400 kHz;

(v) 400-500 kHz;

(vi) 0.5-1.0 MHz;

(vii) 1.0-1.5 MHz;

(viii) 1.5-2.0 MHz;

(ix) 2.0-2.5 MHz;

(x) 2.5-3.0 MHz;

(xi) 3.0-3.5 MHz;

(xii) 3.5-4.0 MHz;

(xiii) 4.0-4.5 MHz;

(xiv) 4.5-5.0 MHz;

(xv) 5.0-5.5 MHz;

(xvi) 5.5-6.0 MHz;

(xvii) 6.0-6.5 MHz;

(xviii) 6.5-7.0 MHz;

(xix) 7.0-7.5 MHz;

(xx) 7.5-8.0 MHz;

(xxi) 8.0-8.5 MHz;

(xxii) 8.5-9.0 MHz;

(xxiii) 9.0-9.5 MHz;

(xxiv) 9.5-10.0 MHz; and

(xxv)>

10.0 MHz.

6. A mass spectrometer as claimed in claim 1, wherein the amplitude of said AC or RF voltage is selected from the group consisting of:

(i)<

50 V peak to peak;

(ii) 50-100 V peak to peak;

(iii) 100-150 V peak to peak;

(iv) 150-200 V peak to peak;

(v) 200-300 V peak to peak;

(vi) 300-400 V peak to peak;

(vii) 400-500 V peak to peak;

(viii) 500-600 V peak to peak;

(ix) 600-700 V peak to peak;

(x) 700-800 V peak to peak;

(xi) 800-900 V peak to peak;

(xii) 900-1000 V peak to peak;

(xiii) 1000-1100 V peak to peak;

(xiv) 1100-1200 V peak to peak;

(xv) 1200-1300 V peak to peak;

(xvi) 1300-1400 V peak to peak;

(xvii) 1400-1500 V peak to peak; and

(xviii)>

1500 V peak to peak.

7. A mass spectrometer as claimed in claim 1, wherein said outer electrode is maintained, in use, at a DC potential selected from the group consisting of:

(i)<

−

500 V;

(ii) −

500 to −

400 V;

(iii) −

400 to −

300 V;

(iv) −

300 to −

200 V;

(v) −

200 to −

100 V;

(vi) −

100 to −

75 V;

(vii) −

75 to −

50 V;

(viii) −

50 to −

25 V;

(ix) −

25 to 0V;

(x) 0V;

(xi) 0-25 V;

(xii) 25-50 V;

(xiii) 50-75 V;

(xiv) 75-100 V;

(xv) 100-200 V;

(xvi) 200-300 V;

(xvii) 300-400 V;

(xviii) 400-500 V;

(xix)>

500 V.

8. A mass spectrometer as claimed in claim 1, wherein said inner electrode is maintained, in use, at a DC potential selected from the group consisting of:

(i) <

−

500 V;

(ii) −

500 to −

400 V;

(iii) −

400 to −

300 V;

(iv) −

300 to −

200 V;

(v) −

200 to −

100 V;

(vi) −

100 to −

75 V;

(vii) −

75 to −

50 V;

(viii) −

50 to −

25 V;

(ix) −

25 to 0V;

(x) 0V;

(xi) 0-25 V;

(xii) 25-50 V;

(xiii) 50-75 V;

(xiv) 75-100 V;

(xv) 100-200 V;

(xvi) 200-300 V;

(xvii) 300-400 V;

(xviii) 400-500 V;

(xix)>

500 V.

9. A mass spectrometer as claimed in claim 1, wherein said outer electrode is maintained at a DC potential which is more positive than the DC potential at which said inner electrode is maintained, in use, by a potential difference selected from the group consisting of:

(i) 0.1-5 V;

(ii) 5-10 V;

(iii) 10-15 V;

(iv) 15-20 V;

(v) 20-25 V;

(vi) 25-30 V;

(vii) 30-40 V;

(viii) 40-50 V; and

(ix)>

50 V.

10. A mass spectrometer as claimed in claim 1, wherein said outer electrode is maintained at a DC potential which is more negative than the DC potential at which said inner electrode is maintained, in use, by a potential difference selected from the group consisting of:

(i) 0.1-5 V;

(ii) 5-10 V;

(iii) 10-15 V;

(iv) 15-20 V;

(v) 20-25 V;

(vi) 25-30 V;

(vii) 30-40 V;

(viii) 40-50 V; and

(ix)>

50 V.

11. A mass spectrometer as claimed in claim 1, wherein said inner electrode comprises a guide wire.

12. A mass spectrometer as claimed in claim 1, wherein at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95% or 100% of said inner electrode comprises a semiconductor or resistive wire and wherein, in use, an axial DC potential gradient is maintained along at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95% or 100% of said inner electrode by applying a DC potential difference across 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95% or 100% of said inner electrode.

13. A mass spectrometer as claimed in claim 1, wherein said inner electrode comprises a cylindrical electrode.

14. A mass spectrometer as claimed in claim 13, wherein said inner electrode comprises a plurality of concentric cylindrical electrodes.

15. A mass spectrometer as claimed in claim 14, wherein, in use, an axial DC potential gradient is maintained along at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95% or 100% of said inner electrode by maintaining at least some of said plurality of concentric cylindrical electrodes at different DC potentials.

16. A mass spectrometer as claimed in claim 1, wherein said inner electrode comprises a plurality of electrodes.

17. A mass spectrometer as claimed in claim 16, wherein in a mode of operation an axial DC potential gradient is maintained along at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95% or 100% of the length of said inner electrode so that ions are urged along at least a portion of said ion guide.

18. A mass spectrometer as claimed in claim 17, wherein said axial DC potential gradient is maintained substantially constant with time as ions pass along said ion guide.

19. A mass spectrometer as claimed in claim 17, wherein said axial DC potential gradient varies with time as ions pass along said ion guide.

20. A mass spectrometer as claimed in claim 1, wherein said outer electrode comprises a plurality of electrodes.

21. A mass spectrometer as claimed in claim 20, wherein in a mode of operation an axial DC potential gradient is maintained along at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95% or 100% of the length of said outer electrode so that ions are urged along at least a portion of said ion guide.

22. A mass spectrometer as claimed in claim 21, wherein said axial DC potential gradient is maintained substantially constant with time as ions pass along said ion guide.

23. A mass spectrometer as claimed in claim 21, wherein said axial DC potential gradient varies with time as ions pass along said ion guide.

24. A mass spectrometer as claimed in claim 1, wherein said ion guide comprises 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or >

30 segments, wherein each segment comprises 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or >

30 electrodes and wherein the electrodes in a segment are maintained at substantially the same DC potential.

25. A mass spectrometer as claimed in claim 24, wherein a plurality of segments are maintained at substantially the same DC potential.

26. A mass spectrometer as claimed in claim 24, wherein each segment is maintained at substantially the same DC potential as the subsequent nth segment wherein n is 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or >

30.

27. A mass spectrometer as claimed in claim 1, wherein ions are constrained axially within said ion guide by a real potential barrier or well.

28. A mass spectrometer as claimed in claim 1, wherein the transit time of ions through said ion guide is selected from the group consisting of:

(i) less than or equal to 20 ms;

(ii) less than or equal to 10 ms;

(iii) less than or equal to 5 ms;

(iv) less than or equal to 1 ms; and

(v) less than or equal to 0.5 ms.

29. A mass spectrometer as claimed in claim 1, wherein in use one or more transient DC voltages or one or more transient DC voltage waveforms are initially provided at a first axial position and are then subsequently provided at second, then third different axial positions along said ion guide.

30. A mass spectrometer as claimed in claim 1, wherein in use one or more transient DC voltages or one or more transient DC voltage waveforms move in use from one end of said ion guide to another end of said ion guide so that ions are urged along said ion guide.

31. A mass spectrometer as claimed in claim 29, wherein said one or more transient DC voltages create:

(i) a potential hill or barrier;

(ii) a potential well;

(iii) multiple potential hills or barriers;

(iv) multiple potential wells;

(v) a combination of a potential hill or barrier and a potential well;

or (vi) a combination of multiple potential hills or barriers and multiple potential wells.

32. A mass spectrometer as claimed in claim 29, wherein said one or more transient DC voltage waveforms comprise a repeating waveform.

33. A mass spectrometer as claimed in claim 32, wherein said one or more transient DC voltage waveforms comprise a square wave.

34. A mass spectrometer as claimed in claim 29, wherein the amplitude of said one or more transient DC voltages or said one or more transient DC voltage waveforms remains substantially constant with time.

35. A mass spectrometer as claimed in claim 29, wherein the amplitude of said one or more transient DC voltages or said one or more transient DC voltage waveforms varies with time.

36. A mass spectrometer as claimed in claim 35, wherein the amplitude of said one or more transient DC voltages or said one or more transient DC voltage waveforms either:

(i) increases with time;

(ii) increases then decreases with time;

(iii) decreases with time;

or (iv) decreases then increases with time.

37. A mass spectrometer as claimed in claim 29, wherein said ion guide comprises an upstream entrance region, a downstream exit region and an intermediate region, wherein:

in said entrance region the amplitude of said one or more transient DC voltages or said one or more transient DC voltage waveforms has a first amplitude;

in said intermediate region the amplitude of said one or more transient DC voltages or said one or more transient DC voltage waveforms has a second amplitude; and

in said exit region the amplitude of said one or more transient DC voltages or said one or more transient DC voltage waveforms has a third amplitude.

38. A mass spectrometer as claimed in claim 37, wherein the entrance and/or exit region comprise a proportion of the total axial length of said ion guide selected from the group consisting of:

(i)<

5%;

(ii) 5-10%;

(iii) 10-15%;

(iv) 15-20%;

(v) 20-25%;

(vi) 25-30%;

(vii) 30-35%;

(viii) 35-40%; and

(ix) 40-45%.

39. A mass spectrometer as claimed in claim 37, wherein said first and/or third amplitudes are substantially zero and said second amplitude is substantially non-zero.

40. A mass spectrometer as claimed in claim 37, wherein said second amplitude is larger than said first amplitude and/or said second amplitude is larger than said third amplitude.

41. A mass spectrometer as claimed in claim 1, wherein one or more transient DC voltages or one or more transient DC voltage waveforms pass in use along said ion guide with a first velocity.

42. A mass spectrometer as claimed in claim 41, wherein said first velocity:

(i) remains substantially constant;

(ii) varies;

(iii) increases;

(iv) increases then decreases;

(v) decreases;

(vi) decreases then increases;

(vii) reduces to substantially zero;

(viii) reverses direction;

or (ix) reduces to substantially zero and then reverses direction.

43. A mass spectrometer as claimed in claim 41, wherein said one or more transient DC voltages or said one or more transient DC voltage waveforms causes ions within said ion guide to pass along said ion guide with a second velocity.

44. A mass spectrometer as claimed in claim 43, wherein the difference between said first velocity and said second velocity is less than or equal to 100 m/s, 90 m/s, 80 m/s, 70 m/s, 60 m/s, 50 m/s, 40 m/s, 30 m/s, 20 m/s, 10 m/s, 5 m/s or 1 m/s.

45. A mass spectrometer as claimed in claim 41, wherein said first velocity is selected from the group consisting of:

(i) 10-250 m/s;

(ii) 250-500 m/s;

(iii) 500-750 m/s;

(iv) 750-1000 m/s;

(v) 1000-1250 m/s;

(vi) 1250-1500 m/s;

(vii) 1500-1750 m/s;

(viii) 1750-2000 m/s;

(ix) 2000-2250 m/s;

(x) 2250-2500 m/s;

(xi) 2500-2750 m/s; and

(xii) 2750-3000 m/s.

46. A mass spectrometer as claimed in claim 43, wherein said second velocity is selected from the group consisting of:

(i) 10-250 m/s;

(ii) 250-500 m/s;

(iii) 500-750 m/s;

(iv) 750-1000 m/s;

(v) 1000-1250 m/s;

(vi) 1250-1500 m/s;

(vii) 1500-1750 m/s;

(viii) 1750-2000 m/s;

(ix) 2000-2250 m/s;

(x) 2250-2500 m/s;

(xi) 2500-2750 m/s; and

(xii) 2750-3000 m/s.

47. A mass spectrometer as claimed in claim 43, wherein said second velocity is substantially the same as said first velocity.

48. A mass spectrometer as claimed in claim 29, wherein said one or more transient DC voltages or said one or more transient DC voltage waveforms has a frequency, and wherein said frequency:

(i) remains substantially constant;

(ii) varies;

(iii) increases;

(iv) increases then decreases;

(v) decreases;

or (vi) decreases then increases.

49. A mass spectrometer as claimed in claim 29, wherein said one or more transient DC voltages or said one or more transient DC voltage waveforms has a wavelength, and wherein said wavelength:

(i) remains substantially constant;

(ii) varies;

(iii) increases;

(iv) increases then decreases;

(v) decreases;

or (vi) decreases then increases.

50. A mass spectrometer as claimed in claim 1, wherein two or more transient DC voltages or two or more transient DC voltage waveforms pass simultaneously along said ion guide.

51. A mass spectrometer as claimed in claim 50, wherein said two or more transient DC voltages or said two or more transient DC voltage waveforms are arranged to move:

(i) in the same direction;

(ii) in opposite directions;

(iii) towards each other;

or (iv) away from each other.

52. A mass spectrometer as claimed in claim 1, wherein one or more transient DC voltages or one or more transient DC voltage waveforms are repeatedly generated and passed in use along said ion guide, and wherein the frequency of generating said one or more transient DC voltages or said one or more transient DC voltage waveforms:

(i) remains substantially constant;

(ii) varies;

(iii) increases;

(iv) increases then decreases;

(v) decreases;

or (vi) decreases then increases.

53. A mass spectrometer as claimed in claim 1, further comprising an ion detector, said ion detector being arranged to be substantially phase locked in use with pulses of ions emerging from the exit of said ion guide.

54. A mass spectrometer as claimed in claim 1, further comprising a Time of Flight mass analyser comprising an electrode for injecting ions into a drift or flight region, said electrode being arranged to be energised in use in a substantially synchronised manner with the pulses of ions emerging from the exit of said ion guide.

55. A mass spectrometer as claimed in claim 1, further comprising an ion trap arranged downstream of said ion guide, said ion trap being arranged to store and/or release ions from said ion trap in a substantially synchronised manner with pulses of ions emerging from the exit of said ion guide.

56. A mass spectrometer as claimed in claim 1, further comprising a mass filter arranged downstream of said ion guide, wherein a mass to charge ratio transmission window of said mass filter is varied in a substantially synchronised manner with pulses of ions emerging from the exit of said ion guide.

57. A mass spectrometer as claimed in claim 1, wherein said ion guide comprises one, two, or more than two entrances for receiving ions and one, two, or more than two exits from which ions emerge from said ion guide.

58. A mass spectrometer as claimed in claim 1, wherein said inner electrode is substantially Y-shaped.

59. A mass spectrometer as claimed in claim 1, wherein said outer electrode is substantially Y-shaped.

60. A mass spectrometer as claimed in claim 1, wherein said ion guide comprises at least one entrance for receiving ions along a first axis and at least one exit from which ions emerge from said ion guide along a second axis, wherein said outer electrode and/or said inner electrode are curved between said entrance and said exit.

61. A mass spectrometer as claimed in claim 60, wherein said ion guide is substantially “

S”

-shaped and/or has a single point of inflexion.

62. A mass spectrometer as claimed in claim 60, wherein said second axis is laterally displaced from said first axis.

63. A mass spectrometer as claimed in claim 1, wherein said ion guide comprises at least one entrance for receiving ions along a first axis and at least one exit from which ions emerge from said ion guide along a second axis, wherein said second axis is inclined at an angle θ

to said first axis and wherein θ

>

0°

.

64. A mass spectrometer as claimed in claim 63, wherein θ

falls within the range;

(i)<

10°

;

(ii) 10-20°

;

(iii) 20-30°

;

(iv) 30-40°

;

(v) 40-50°

;

(vi) 50-60°

;

(vii) 60-70°

;

(viii) 70-80°

;

(ix) 80-90°

;

(x) 90-100°

;

(xi) 100-110°

;

(xii) 110-120°

;

(xiii) 120-130°

;

(xiv) 130-140°

;

(xv) 140-150°

;

(xvi) 150-160°

;

(xvii) 160-170°

; and

(xviii) 170-180°

.

65. A mass spectrometer as claimed in claim 1, wherein at least a portion of said ion guide either:

(i) varies in size and/or shape along the length of said ion guide;

or (ii) has a width and/or height which progressively tapers in size.

66. A mass spectrometer as claimed in claim 1, wherein said inner electrode is arranged offset from the central axis of said outer electrode.

67. A mass spectrometer as claimed in claim 1, wherein the distance between said inner electrode and said outer electrode varies along at least a portion of said ion guide.

68. A mass spectrometer as claimed in claim 1, further comprising an ion source, said ion source being selected from the group consisting of:

(i) an Electrospray (“

ESI”

) ion source;

(ii) an Atmospheric Pressure Chemical Ionisation (“

APCI”

) ion source;

(iii) an Atmospheric Pressure Photo Ionisation (“

APPI”

) ion source;

(iv) a Matrix Assisted Laser Desorption Ionisation (“

MALDI”

) ion source;

(v) a Laser Desorption Ionisation (“

LDI”

) ion source;

(vi) an Inductively Coupled Plasma (“

ICP”

) ion source;

(vii) an Electron Impact (“

EI”

) ion source;

(viii) a Chemical Ionisation (“

CI”

) ion source;

(ix) a Fast Atom Bombardment (“

FAB”

) ion source; and

(x) a Liquid Secondary Ions Mass Spectrometry (“

LSIMS”

) ion source.

69. A mass spectrometer as claimed in claim 1, further comprising a pulsed ion source.

70. A mass spectrometer as claimed in claim 1, further comprising a continuous ion source.

71. A mass spectrometer as claimed in claim 1, said ion guide having an entrance for receiving ions and an exit from which ions are released, wherein said entrance and/or exit of the ion guide are maintained at a potential so that ions are reflected at said entrance and/or exit.

72. A mass spectrometer as claimed in claim 71, further comprising at least one ring lens, plate electrode or grid electrode arranged at said entrance and/or exit of said ion guide and wherein said at least one ring lens, plate electrode or grid electrode is arranged to be maintained at a potential so that ions are reflected at said entrance and/or exit.

73. A mass spectrometer as claimed in claim 72, wherein an AC or RF voltage and/or a DC voltage is supplied to said at least one ring lens, plate electrode or grid electrode so that ions are reflected at said entrance and/or exit.

74. A mass spectrometer as claimed in claim 1, further comprising a mass analyser arranged downstream of said ion guide, said mass analyser selected from the group consisting of:

(i) a Time of Flight mass analyser;

(ii) a quadrupole mass analyser;

(iii) a Fourier Transform Ion Cyclotron Resonance (“

FTICR”

) mass analyser;

(iv) a 2D (linear) quadrupole ion trap;

(v) a 3D (Paul) quadrupole ion trap; and

(vi) a magnetic sector mass analyser.

75. A mass spectrometer as claimed in claim 1, wherein in a mode of operation said ion guide is maintained in use at a pressure selected from the group consisting of:

(i) greater than or equal to 0.0001 mbar;

(ii) greater than or equal to 0.0005 mbar;

(iii) greater than or equal to 0.001 mbar;

(iv) greater than or equal to 0.005 mbar;

(v) greater than or equal to 0.01 mbar;

(vi) greater than or equal to 0.05 mbar;

(vii) greater than or equal to 0.1 mbar;

(viii) greater than or equal to 0.5 mbar;

(ix) greater than or equal to 1 mbar;

(x) greater than or equal to 5 mbar; and

(xi) greater than or equal to 10 mbar.

76. A mass spectrometer as claimed in claim 1, wherein in a mode of operation said ion guide is maintained in use at a pressure selected from the group consisting of:

(i) less than or equal to 10 mbar;

(ii) less than or equal to 5 mbar;

(iii) less than or equal to 1 mbar;

(iv) less than or equal to 0.5 mbar;

(v) less than or equal to 0.1 mbar;

(vi) less than or equal to 0.05 mbar;

(vii) less than or equal to 0.01 mbar;

(viii) less than or equal to 0.005 mbar;

(ix) less than or equal to 0.001 mbar;

(x) less than or equal to 0.0005 mbar; and

(xi) less than or equal to 0.0001 mbar.

77. A mass spectrometer as claimed in claim 1, wherein in a mode of operation said ion guide is maintained in use at a pressure selected from the group consisting of:

(i) between 0.0001 and 10 mbar;

(ii) between 0.0001 and 1 mbar;

(iii) between 0.0001 and 0.1 mbar;

(iv) between 0.0001 and 0.01 mbar;

(v) between 0.0001 and 0.001 mbar;

(vi) between 0.001 and 10 mbar;

(vii) between 0.001 and 1 mbar;

(viii) between 0.001 and 0.1 mbar;

(ix) between 0.001 and 0.01 mbar;

(x) between 0.01 and 10 mbar;

(xi) between 0.01 and 1 mbar;

(xii) between 0.01 and 0.1 mbar;

(xiii) between 0.1 and 10 mbar;

(xiv) between 0.1 and 1 mbar; and

(xv) between 1 and 10 mbar.

78. A mass spectrometer as claimed in claim 1, wherein a mode of operation said ion guide is maintained in use at a pressure selected from the group consisting of:

(i) greater than or equal to 1×

10<sup>−

7</sup> mbar;

(ii) greater than or equal to 5×

10<sup>−

7</sup> mbar;

(iii) greater than or equal to 1×

10<sup>−

6</sup> mbar;

(iv) greater than or equal to 5×

10<sup>−

6</sup> mbar;

(v) greater than or equal to 1×

10<sup>−

5</sup> mbar; and

(vi) greater than or equal to 5×

10<sup>−

5</sup> mbar.

79. A mass spectrometer as claimed in claim 1, wherein in a mode of operation said ion guide is maintained in use at a pressure selected from the group consisting of:

(i) less than or equal to 1×

10<sup>−

4</sup> mbar;

(ii) less than or equal to 5×

10<sup>−

5</sup> mbar;

(iii) less than or equal to 1×

10<sup>−

5</sup> mbar;

(iv) less than or equal to 5×

10<sup>−

6</sup> mbar;

(v) less than or equal to 1×

10<sup>−

6</sup> mbar;

(vi) less than or equal to 5×

10<sup>−

7</sup> mbar; and

(vii) less than or equal to 1×

10<sup>−

7</sup> mbar.

80. A mass spectrometer as claimed in claim 1, wherein in a mode of operation said ion guide is maintained, in use, at a pressure selected from the group consisting of:

(i) between 1×

10<sup>−

7</sup> and 1×

10<sup>−

4</sup> mbar;

(ii) between 1×

10<sup>−

7</sup> and 5×

10<sup>−

5</sup> mbar;

(iii) between 1×

10<sup>−

7</sup> and 1×

10<sup>−

5</sup> mbar;

(iv) between 1×

10<sup>−

7</sup> and 5×

10<sup>−

6</sup> mbar;

(v) between 1×

10<sup>−

7</sup> and 1×

10<sup>−

6</sup> mbar;

(vi) between 1×

10<sup>−

7</sup> and 5×

10<sup>−

7</sup> mbar;

(vii) between 5×

10<sup>−

7</sup> and 1×

10<sup>−

4</sup> mbar;

(viii) between 5×

10<sup>−

7</sup> and 5×

10<sup>−

5</sup> mbar;

(ix) between 5×

10<sup>−

7</sup> and 1×

10<sup>−

5</sup> mbar;

(x) between 5×

10<sup>−

7</sup> and 5×

10<sup>−

6</sup> mbar;

(xi) between 5×

10<sup>−

7</sup> and 1×

10<sup>−

6</sup> mbar;

(xii) between 1×

10<sup>−

6</sup> mbar and 1×

10<sup>−

4</sup> mbar;

(xiii) between 1×

10<sup>−

6</sup> and 5×

10<sup>−

5</sup> mbar;

(xiv) between 1×

10<sup>−

6</sup> and 1×

10<sup>−

5</sup> mbar;

(xv) between 1×

10<sup>−

6</sup> and 5×

10<sup>−

6</sup> mbar;

(xvi) between 5×

10<sup>−

6</sup> mbar and 1×

10<sup>−

4</sup> mbar;

(xvii) between 5×

10<sup>−

6</sup> and 5×

10<sup>−

5</sup> mbar;

(xviii) between 5×

10<sup>−

6</sup> and 1×

10<sup>−

5</sup> mbar;

(xix) between 1×

10<sup>−

5</sup> mbar and 1×

10<sup>−

4</sup> mbar;

(xx) between 1×

10<sup>−

5</sup> and 5×

10<sup>−

5</sup> mbar; and

(xxi) between 5×

10<sup>−

5</sup> and 1×

10<sup>−

4</sup> mbar.

81. A mass spectrometer comprising an ion guide, said ion guide comprising a guide wire, cylindrical or rod electrode and an outer cylindrical electrode wherein, in use, both an AC and a DC potential difference is maintained between said guide wire, cylindrical or rod electrode and said outer cylindrical electrode.

82. A method of mass spectrometry, comprising:

guiding ions along an ion guide comprising an outer electrode and an inner electrode disposed within said outer electrode;

maintaining said inner and outer electrodes at a DC potential difference such that ions experience a first radial force towards said inner electrode; and

applying an AC or RF voltage to said inner and/or said outer electrodes so that ions experience a second radial force towards said outer electrode.

83. A mass spectrometer comprising:

an ion guide comprising a guide wire held centrally in an electrically conductive cylindrical tube electrode wherein both AC and DC voltages are applied, in use, between the guide wire and the cylindrical tube electrode in order to radially retain ions whilst said ions are being transported axially through said ion guide.

84. A mass spectrometer as claimed in claim 83, wherein said guide wire comprises a semiconductor or resistive wire so that an axial DC field is maintained, in use, along said ion guide by the application of a DC voltage between the ends of said guide wire.

85. A mass spectrometer comprising:

an ion guide comprising a guide wire held centrally in a plurality of outer concentric cylindrical electrodes wherein both AC and DC voltages are applied, in use, between the guide wire and the plurality of outer concentric cylindrical electrodes in order to radially retain ions whilst said ions are being transported axially through said ion guide.

86. A mass spectrometer as claimed in claim 85, wherein an axial DC field is maintained, in use, along said ion guide by the application of DC voltages to said plurality of outer cylindrical electrodes.

87. A mass spectrometer as claimed in claim 85, wherein travelling potential wave functions are applied, in use, to said outer cylindrical electrodes to assist in ion transmission.

88. A mass spectrometer comprising:

an ion guide comprising a guide wire held centrally in an electrically conductive cylindrical tube electrode wherein both AC and DC voltages are applied, in use, between the guide wire and the cylindrical tube electrode and wherein ions are arranged, in use, to impact the inside wall of said cylindrical tube electrode or the guide wire to produce secondary ion disassociation by adjusting the AC or DC voltages.

89. A mass spectrometer comprising:

an ion guide comprising a guide wire held centrally in an electrically conductive cylindrical tube electrode wherein both AC and DC voltages are applied, in use, between the guide wire and the cylindrical tube electrode and wherein said AC voltage or said DC voltage is adjusted so as to cause an increase in the internal energy of ions within said ion guide thereby inducing collisional fragmentation or collisional induced disassociation of said ions.

90. A mass spectrometer comprising:

an ion guide comprising an inner cylindrical electrode held centrally in an electrically conductive cylindrical tube electrode wherein both AC and DC voltages are applied, in use, between the inner cylindrical electrode and the cylindrical tube electrode in order to radially retain ions whilst said ions are being transported axially through said ion guide.

91. A mass spectrometer comprising:

an ion guide comprising a guide wire held centrally in an electrically conductive cylindrical tube electrode wherein both AC and DC voltages are applied, in use, between the guide wire and the cylindrical tube electrode in order to radially retain ions whilst said ions are being transported axially through said ion guide and wherein said guide wire splits into two or more wires.

92. A mass spectrometer as claimed in claim 91, wherein different AC or DC voltages are applied to said two or more wires.

93. A mass spectrometer comprising:

an ion guide comprising a guide wire held centrally in an electrically conductive cylindrical tube electrode wherein both AC and DC voltages are applied, in use, between the guide wire and the cylindrical tube electrode in order to radially retain ions whilst said ions are being transported axially through said ion guide and wherein said guide wire is not straight.

94. A mass spectrometer as claimed in claim 93, wherein said guide wire is circular.

95. A mass spectrometer comprising an ion guide, said ion guide comprising a Y-shaped outer cylindrical electrode and a Y-shaped inner guide wire electrode, wherein in use said outer electrode and said inner electrode are supplied with both an AC voltage and a DC voltage and wherein said ion guide is arranged so that an ion beam is split or ion beams are joined.

96. A mass spectrometer comprising:

an ion guide comprising a guide wire held centrally in an electrically conductive cylindrical tube electrode wherein both AC and DC voltages are applied, in use, between the guide wire and the cylindrical tube electrode in order to radially retain ions whilst said ions are being transported axially through said ion guide, said ion guide further comprising a ring lens, plate or grid and wherein an additional DC or AC voltage is applied, in use, to said ring lens, plate or grid so that ions are reflected backwards and are trapped or stored within said ion guide.