Adaptation sites of porcine reproductive and respiratory syndrome virus

• US 7,312,030 B2
• Filed: 04/04/2003
• Issued: 12/25/2007
• Est. Priority Date: 04/05/2002
• Status: Active Grant

• Current Assignee: Boehringer Ingelheim Vetmedica GmbH (C.H. Boehringer Sohn AG & Co. KG)
• Original Assignee: Boehringer Ingelheim Vetmedica GmbH (C.H. Boehringer Sohn AG & Co. KG)
• Inventors: Meulenberg, Johanna Jacoba M., van Rijn, Petrus Antonius
• Primary Examiner(s): Campell; Bruce R.
• Assistant Examiner(s): Boesen; Agnieszka
• Application Number:
  US10/407,822
• Publication Number:
  US 20030219732A1
• Time in Patent Office: 1,726 Days
• Field of Search: 435/69.1
• US Class Current: 435/5
• CPC Class Codes: A61K 2039/522 avirulent or attenuated
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 2770/10021 Viruses as such, e.g. new isolates, mutants or their genomic sequences
• CPC Class Codes: C12N 2770/10022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2770/10061 Methods of inactivation or attenuation
• CPC Class Codes: C12N 2770/10064 by serial passage
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00

Abstract

A method for determining the capability of an arterivirus to replicate in a permissive cell is disclosed. The method includes determining an amino acid at a position that corresponds to an amino acid of a protein of a porcine reproductive and respiratory syndrome virus. The invention also discloses a method for determining the capability of an arterivirus to replicate in a green monkey cell line. The invention further discloses a method for producing arterivirus in a green monkey cell line wherein the virulence of the arterivirus is maintained and the virus yield is increased. Methods for determining the attenuation of an arterivirus and for attenuating the virulence of the arterivirus by changing amino acids are further disclosed.

Claims

1. A method for increasing the capability of an Arterivirus to replicate in a permissive cell, said method comprising:

determining amino acids of the Arterivirus at positions that correspond to amino acid positions 75-107 of GP2a (SEQ. ID. NO.

     6) of PRRSV isolate I-1102; and

changing at least one of the determined amino acids of the Arterivirus to a different amino acid, wherein at least one of the determined amino acids of the Arterivirus corresponds to position 88 of GP2a of PRRSV isolate I-1102, and wherein the determined amino acid of the Arterivirus that corresponds to position 88 of GP2a of PRRV isolate I-1102 is changed from a valine to another amino acid.

2. The method according to claim 1, wherein the determined amino acid of the Arterivirus that corresponds to position 88 of GP2a of PRRV isolate I-1102 is changed from valine to phenylalanine.

3. The method according to claim 1, wherein the permissive cell is a green monkey cell.