Photocleavable bioreactive agents
First Claim
1. A method, comprising,a) providing a mixture, said mixture comprising a conjugate, said conjugate comprising a bioreactive agent bound to a substrate, wherein said substrate comprises a nucleic acid, said bioreactive agent comprises a photocleavable moiety and a detectable moiety, wherein said photocleavable moiety is positioned between said detectable moiety and said substrate;
- b) separating said conjugate from said mixture to create a separated conjugate; and
c) treating said separated conjugate with electromagnetic radiation releasing said substrate from said detectable moiety under conditions so as to create released substrate.
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Abstract
This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. Useful compositions include biological agents such as nucleic acids, proteins, lipids and cytokines. Conjugates can also be used to monitor the pathway and half-life of pharmaceutical composition in vivo and for diagnostic, therapeutic and prophylactic purposes. The invention also relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and nearly any individual substance in a complex background of substances.
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Citations
16 Claims
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1. A method, comprising,
a) providing a mixture, said mixture comprising a conjugate, said conjugate comprising a bioreactive agent bound to a substrate, wherein said substrate comprises a nucleic acid, said bioreactive agent comprises a photocleavable moiety and a detectable moiety, wherein said photocleavable moiety is positioned between said detectable moiety and said substrate; -
b) separating said conjugate from said mixture to create a separated conjugate; and c) treating said separated conjugate with electromagnetic radiation releasing said substrate from said detectable moiety under conditions so as to create released substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 11, 14, 16)
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8. A method, comprising
a) providing a mixture, said mixture comprising a conjugate, said conjugate comprising a bioreactive agent bound to a substrate, said bioreactive agent comprising a biotinyl moiety linked to a photoreactive moiety with a spacer arm to form a photocleavable biotinyl moiety, wherein said photocleavable moiety is positioned between said biotinyl moiety and said substrate; -
b) separating said conjugate from said mixture to create a separated conjugate; and c) treating said separated conjugate with electromagnetic radiation releasing said substrate from said biotinyl moiety under conditions so as to create released substrate, wherein said released substrate is unmodified. - View Dependent Claims (9, 10, 12, 13, 15)
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Specification