Devices and methods for biochip multiplexing

• US 7,312,087 B2
• Filed: 01/11/2001
• Issued: 12/25/2007
• Est. Priority Date: 01/11/2000
• Status: Expired due to Term

• Current Assignee: Roche Molecular Systems Inc (Roche Holding AG)
• Original Assignee: Clinical Micro Sensors Inc. (Roche Holding AG)
• Inventors: Duong, Hau H., Blackburn, Gary, Kayyem, Jon F., O'Connor, Stephen D., Olsen, Gary T., Pietri, Robert, Terbrueggen, Robert H.
• Primary Examiner(s): SINES, BRIAN J
• Application Number:
  US09/760,384
• Publication Number:
  US 20070189921A1
• Time in Patent Office: 2,539 Days
• Field of Search: 422/50, 422/55, 422/56, 422/58, 422/68.1, 422/81, 422/82.01, 422/82.05, 436/8, 436/43, 436/174, 436/501, 436/63, 436/64, 436/66, 436/149, 436/150, 73/10.1, 137/1, 137/87.01, 137/154, 251/205, 438/1, 205/775, 205/792, 205/793.5, 204/164, 204/461, 204/193, 204/194, 204/400, 204/403.01, 204/403.02, 204/403.03, 204/406, 204/407
• US Class Current: 436/149
• CPC Class Codes: G01N 2035/00158 Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
• CPC Class Codes: Y10T 436/11 Automated chemical analysis

Abstract

The invention is directed to devices and methods that allow for simultaneous multiple biochip analysis. The method of analyzing the plurality of biochips includes inserting a first biochp into a first station of an analysis device, inserting a second biochip into a second station of the analysis device, wherein each of the first and second biochips include a substrate, the substrates including an array of detection electrodes, each electrode including a different capture binding ligand, a different target analyte, and a label, and a plurality of electrical contracts, detecting current as an indication of the presence of the labels on the first biochip, and detecting current as an indication of the presence of the labels on the first second biochip. The devices and method may be used with multiple cartridges comprising biochips comprising arrays, such as nucleic acid arrays, and allow for high throughput analysis of samples.

Claims

1. A method of analyzing a plurality of biochips comprising

a) inserting a first biochip into a first station of an analysis device;

b) inserting a second biochip into a second station of the analysis device, wherein each of said first and second biochips comprise a substrate comprising;

an array of detection electrodes, each comprising;

i) a different capture binding ligand;

ii) a different target analyte; and

iii) a label; and

;

a plurality of electrical contacts;

c) detecting current as an indication of the presence of said labels on said first biochip; and

d) detecting current as an indication of the presence of said labels on said second biochip.

2. A method according to claim 1, wherein said capture binding ligands are nucleic acid capture probes, said target analytes are target nucleic acid sequences, and said nucleic acid capture probes hybridize to said target nucleic acid sequences to form hybridization complexes.

3. A method according to claim 2, wherein said hybridization complexes comprise said capture probes hybridized to said target sequences, respectively.

4. A method according to claim 2, wherein said labels are covalently attached to said target sequences.

5. A method according to claim 1 or 2, wherein said labels are hybridization indicators.

6. A method according to claim 5, wherein said hybridization indicators are intercalators.

7. A method according to claim 2, wherein said target sequences each comprise a first domain and a second domain, said hybridization complexes each comprise:

a) said capture probes hybridized to said first domains of said target sequences; and

b) label probes hybridized to said second domains of said target sequences.

8. A method according to claim 7 wherein said label probes each comprise three or more covalently attached labels.

9. A method according to claim 1, 2 or 8 wherein said labels are electron transfer moieties (ETMs).

10. A method according to claim 9 wherein said ETMs are transition metal complexes.

11. A method according to claim 10 wherein said transition metal complexes are metallocenes.

12. A method according to claim 1, further comprising:

a) receiving detection information from said first biochip at a processor; and

b) receiving detection information from said second biochip at said processor.

13. A method according to claim 12, further comprising analyzing said received detection information.

14. A method according to claim 13, wherein said analyzing step comprises analyzing higher harmonic frequencies.