Methods and materials for optimization of electronic transportation and hybridization reactions
First Claim
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1. A method for the transport of a nucleic acid to an electrode site comprising the steps of:
- providing a nucleic acid and a low conductivity, zwitterionic buffer with a reducing agent to an active electronic system comprising an electrode;
applying current and voltage to the electrode to effect electrophoretic transportation of the nucleic acid towards the electrode; and
hybridizing the nucleic acid with a probe located at the electrode, whereby the local pH above the electrode is below the pH of the buffer at its isoelectric point.
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Abstract
Methods for the transport and hybridization of a nucleic acid on an electrode device by providing a low conductivity buffer with a reducing agent to the device. The low conductivity buffer may also contain a zwitterion. A current and voltage is applied to a location of the device to effect electrophoretic transportation of the nucleic acid towards the location. The nucleic acid is then hybridized to a nucleic probed located at the location. The reducing agent in the low conductivity buffer may also be acting as a chaotropic agent.
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19 Claims
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1. A method for the transport of a nucleic acid to an electrode site comprising the steps of:
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providing a nucleic acid and a low conductivity, zwitterionic buffer with a reducing agent to an active electronic system comprising an electrode; applying current and voltage to the electrode to effect electrophoretic transportation of the nucleic acid towards the electrode; and hybridizing the nucleic acid with a probe located at the electrode, whereby the local pH above the electrode is below the pH of the buffer at its isoelectric point. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification