Electrochemical biosensor strip

• US 7,316,766 B2
• Filed: 05/27/2005
• Issued: 01/08/2008
• Est. Priority Date: 05/27/2005
• Status: Active Grant

• Current Assignee: TaiDoc Technology Corporation
• Original Assignee: TaiDoc Technology Corporation
• Inventors: Lee, Tsai-Yun, Chen, Chun-Tung, Wu, Chia-Chi, Wu, Shu-Mei, Chen, Chao-Wang, Pi, Chun-Hui
• Primary Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US11/138,403
• Publication Number:
  US 20060266645A1
• Time in Patent Office: 956 Days
• Field of Search: 205/777.5, 205/778, 205/792, 20440301-40315
• US Class Current: 204/403.01
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

An electrochemical biosensor strip has a base, an electrode system, an optional spacer and a cover. The electrode system is laid on the base. The spacer is laid on the electrode system and exposes a portion of the electrode system for electrical connection with a meter and a different portion of the electrode system for application of a test reagent. The cover is covered on the spacer to form a cavity. Between the test reagent and the base, a hydrophilic layer is laid between them for increasing the binding effect of the test reagent on the base. The hydrophilic layer is laid on the area excluding from the electrode system and laid on the electrode system about 50% to 0% of the electrode system corresponding to the test reagent. The hydrophilic layer will not interfere with signal transmission of the electrode system so the test is more accurate.

Claims

1. An electrochemical biosensor strip, comprising:

a base;

an electrode system laid on the base and a portion of the electrode system exposed for an electrical connection adapted to a meter and a predetermined portion of the electrode system exposed for applying a test reagent;

a cover cooperating with the base to form a cavity for drawing an analyte-containing fluid therein to react with the test reagent; and

a hydrophilic layer laid under the test reagent and laid on an area of the base excluded from the electrode system corresponding to the test reagent and also laid on the electrode system about 50% to 0% of the electrode system corresponding to the test reagent, wherein the hydrophilic layer has a size corresponding to the test reagent.

2. The electrochemical biosensor strip as claimed in claim 1, wherein the hydrophilic layer is water soluble cellulose, surfactant, thickening agent, organic polar compound or a combination thereof.

3. The electrochemical biosensor strip as claimed in claim 1, wherein the hydrophilic layer is methylcellulose, carboxymethyl cellulose, methylhydroxyethylcellulose, methylhydroxypropylcellulose, hydroxyethylcellulose, hydroxyethylcarboxymethylcellulose, carboxymethylhydroxyethylcellulose or a combination thereof.

4. The electrochemical biosensor strip as claimed in claim 1, wherein the electrode system comprises at least one working electrode and the hydrophilic layer laid both on an area of the base and the electrode system excluded from the working electrode corresponding to the test reagent and also laid on the working electrode about 50% to 0% of the working electrode corresponding to the test reagent.

5. The electrochemical biosensor strip as claimed in claim 4, wherein the hydrophilic layer laid on the working electrode is at least one strip-like shape.

6. The electrochemical biosensor strip as claimed in claim 5, wherein the hydrophilic layer laid on the working electrode is at least one strip-like shape formed on a middle of the working electrode.

7. The electrochemical biosensor strip as claimed in claim 4, wherein the hydrophilic layer laid on the area excluded from the working electrode is a plurality of strip-like shapes formed under the test reagent.

8. The electrochemical biosensor strip as claimed in claim 1, wherein the cover further comprises a concave unit formed corresponding to the test reagent and outside of the test reagent and the concave unit is semicircle-shape or semiellipse-shape.

9. The electrochemical biosensor strip as claimed in claim 1, further comprising a spacer covered on the electrode system to expose the portion of the electrode system for an electrical connection adapted to a meter, having an opening formed in one side of the spacer to expose the predetermined portion of the electrode system for applying the test reagent.

10. The electrochemical biosensor strip as claimed in claim 9, wherein the spacer further comprises an exhausting channel formed extendedly from the opening and opened to another side of the spacer.

11. The electrochemical biosensor strip as claimed in claim 9, wherein the cover further has a hole corresponding to the test reagent.

12. The electrochemical biosensor strip as claimed in claim 11, wherein the cover further has a channel formed corresponding to the opening of the spacer and communicating to the hole.

13. The electrochemical biosensor strip as claimed in claim 1, further comprising a spacer covered on the electrode system to expose the portion of the electrode system for an electrical connection adapted to a meter, having an opening formed in one end of the spacer to expose the predetermined portion of the electrode system for applying the test reagent.

14. The electrochemical biosensor strip as claimed in claim 13, wherein the spacer further has an exhausting channel communicated with the opening to form a T-shape.

15. The electrochemical biosensor strip as claimed in claim 14, further comprising an insulating substance laid on the electrode system which has an opening formed corresponding to the opening of the spacer.

16. The electrochemical biosensor strip as claimed in claim 1, further comprising a rough unit formed on the base corresponding to the test reagent and adjacent to the electrode system.

17. The electrochemical biosensor strip as claimed in claim 16, wherein the rough unit is a plurality of lines.

18. The electrochemical biosensor strip as claimed in claim 1, wherein the electrode system comprises at least three electrodes and a short circuit is formed between two selected electrodes of the at least three electrodes.

19. The electrochemical biosensor strip as claimed in claim 1, wherein the electrode system comprises a silver layer laid on the base and a carbon layer laid on the silver layer and the silver layer has a X-shaped unit formed on the base and between the electrodes and the carbon layer has an arrowhead shape unit formed corresponding to the X-shaped unit.