Fluid analytical devices

• US 7,320,776 B2
• Filed: 07/05/2005
• Issued: 01/22/2008
• Est. Priority Date: 12/22/2004
• Status: Active Grant

• Current Assignee: Apex Biotechnology Corporation, Industrial Technology Research Institute
• Original Assignee: Apex Biotechnology Corporation, Industrial Technology Research Institute
• Inventors: Hsieh, Wen-Pin, Jian, Jian-Je, Liau, Iany-H, Chuang, Chin-Tang, Yang, Ke-Shieng
• Primary Examiner(s): Warden; Jill
• Assistant Examiner(s): TURK, NEIL N
• Application Number:
  US11/175,131
• Publication Number:
  US 20060133958A1
• Time in Patent Office: 931 Days
• Field of Search: 436/45
• US Class Current: 422/72
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor

Abstract

A fluid analytical device for separating and analyzing a fluid including a first component and a second component. The specific weight of the first component exceeds that of the second component. A rotating base includes a receiving chamber, a first separation well, a second separation well, a first connection channel, a second connection channel, and a vent channel. The receiving chamber receives the fluid. The first connection channel connects the receiving chamber to the first separation well. The second connection channel connects the first separation well to the second separation well. The vent channel is connected to the receiving chamber. The distance from the first separation well to the receiving chamber exceeds that from the second separation well to the receiving chamber. When the rotating base rotates, the first and second components of the fluid flow into the first and second separation wells by centrifugal force, respectively.

Claims

1. A fluid analytical device for separating and analyzing a fluid comprising a first component and a second component, wherein the specific weight of the first component exceeds that of the second component, comprising:

a rotating base comprising a receiving chamber, a first separation well, a second separation well, a first connection channel, a second connection channel, and a vent channel, wherein the receiving chamber receives the fluid, the first connection channel directly connects the receiving chamber to the first separation well, the second connection channel directly connects the first separation well to the second separation well, the vent channel is directly connected between the second separation well and the receiving chamber, the minimum distance between edges of the first separation well and the receiving chamber exceeds the minimum distance between edges of the second separation well and the receiving chamber,

whereby, when the rotating base rotates, the first component of the fluid flows into the first separation well via the first connection channel by centrifugal force and friction while the second component flows into the second separation well via the first connection channel, first separation well, and second connection channel by the difference of componential vectors of the centrifugal force and the specific weight of the second component less than that of the first component.

2. The fluid analytical device as claimed in claim 1, wherein the rotating base further comprises an input hole connected to the receiving chamber, and the fluid is input to the receiving chamber via the input hole.

3. The fluid analytical device as claimed in claim 1, wherein a first included angle between the first and second connection channels is between 0°

and 90°

.

4. The fluid analytical device as claimed in claim 1, wherein the proportion of the cross-sectional area of the first connection channel to that of the second connection channel exceeds or equals 1.

5. The fluid analytical device as claim in claim 1 wherein the cross-sectional area of the vent channel is less than 1 mm².

6. The fluid analytical device as claimed in claim 1, wherein the fluid further comprises a third component, the rotating base further comprises a third separation well and a third connection channel, the specific weight of the second component exceeds that of the third component, the third connection channel connects the second separation well to the third separation well, the minimum distance between the edges of the second separation well and the receiving chamber exceeds the minimum distance between edges of the third separation well and the receiving chamber, whereby, when the rotating base rotates, the third component flows into the third separation well via the first connection channel, first separation well, second connection channel, second separation well, and third connection channel by the centrifugal force.

7. The fluid analytical device as claimed in claim 6, wherein the rotating base further comprises an input hole connected to the receiving chamber, and the fluid is input to the receiving chamber via the input hole.

8. The fluid analytical device as claimed in claim 6, wherein the proportion of the minimum distance between the edges of the first separation well and the receiving chamber to the minimum distance between the edges of the second separation well and the receiving chamber is between 1 and 8, and the proportion of the minimum distance between the edges of the first separation well and the receiving chamber to the minimum distance between the edges of the third separation well and the receiving chamber is between 1 and 10.

9. The fluid analytical device as claimed in claim 6, wherein a first included angle between the first and second connection channels is between 0°

and 90°

, a second included angle between the second and third connection channels is less than 180°

.

10. The fluid analytical device as claimed in claim 6, wherein the proportion of the cross-sectional area of the first connection channel to that of the second connection channel is between 1 and 5, and the proportion of the cross-sectional area of the first connection channel to that of the third connection channel is between 1 and 8.

11. The fluid analytical device as claim in claim 9, wherein the cross-sectional area of the vent channel is less than 1 mm².

12. The fluid analytical device as claimed in claim 1, wherein the receiving chamber is disposed on a rotational center of the rotating base.