Method for the identification and/or the quantification of a target compound obtained from a biological sample upon chips
DCFirst Claim
1. A method for the identification and/or the quantification of a target compound obtained from a sample, comprising the steps of:
- putting into contact the target compound with a capture molecule in order to allow a specific binding between said target compound with said capture molecule, said capture molecule being fixed upon a surface of a solid support according to an array comprising discrete regions with a density of at least 20 of said discrete regions per cm2, each of said discrete regions being fixed with one species of capture molecules;
performing a catalytic reduction of a metal present in solution leading to formation of a metallic precipitate in one or more of said discrete regions;
determining the possible presence and quantification of precipitate(s) in said discrete region(s); and
correlating the presence and quantification of the precipitate(s) at the discrete region(s) with the identification and/or a quantification of said target compound.
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Abstract
The present invention is related to a method for the identification and/or the quantification of a target compound obtained from a sample, preferably a biological sample, comprising the steps of putting into contact the target compound with a capture molecule in order to allow a specific binding between the target compound with a capture molecule, the capture molecule being fixed upon a surface of a solid support according to an array comprising a density of at least 20 discrete regions per cm2, each of the discrete regions being fixed with one species of capture molecules, performing a reaction leading to a precipitate formed at the location of the binding, determining the possible presence of precipitate(s) in discrete region(s), and correlating the presence of the precipitate(s) at the discrete region(s) with the identification and/or a quantification of the target compound.
24 Citations
32 Claims
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1. A method for the identification and/or the quantification of a target compound obtained from a sample, comprising the steps of:
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putting into contact the target compound with a capture molecule in order to allow a specific binding between said target compound with said capture molecule, said capture molecule being fixed upon a surface of a solid support according to an array comprising discrete regions with a density of at least 20 of said discrete regions per cm2, each of said discrete regions being fixed with one species of capture molecules; performing a catalytic reduction of a metal present in solution leading to formation of a metallic precipitate in one or more of said discrete regions; determining the possible presence and quantification of precipitate(s) in said discrete region(s); and
correlating the presence and quantification of the precipitate(s) at the discrete region(s) with the identification and/or a quantification of said target compound. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 31, 32)
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16. A diagnostic and/or quantification apparatus comprising:
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a solid support comprising an array comprising at least 20 discrete regions per cm2 comprising multiple species of capture molecules, each of said discrete regions being fixed with one species of capture molecule which recognizes a target compound, wherein binding of said target compound to one or more of said multiple species of capture molecules leads to a catalytic reduction of a metal present in solution, and a formation of a metallic precipitate in one or more of said discrete region(s); a detection and quantification device for detecting and quantifying said precipitate in said discrete region(s); and a computer programmed to collect the results obtained from said detection and quantification device. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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Specification