Biosensor and measuring method using the same

• US 7,323,098 B2
• Filed: 09/03/2003
• Issued: 01/29/2008
• Est. Priority Date: 09/03/2002
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Inventors: Yoshioka, Toshihiko, Miyashita, Mariko
• Primary Examiner(s): Noguerola; Alex
• Application Number:
  US10/653,257
• Publication Number:
  US 20040040866A1
• Time in Patent Office: 1,609 Days
• Field of Search: 20440301-40315, 204409-412, 205/777.5, 205/778, 205/792
• US Class Current: 205/777.5
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

A biosensor capable of measuring the concentration of one or more specific substances in one or more sample solutions almost simultaneously is provided. The biosensor comprises a plurality of sensor units, and each of the sensor units comprises an electrode system including a working electrode and a counter electrode on an insulating base plate and a reagent system including an oxidoreductase and an electron mediator. The biosensor is so configured that sample solutions supplied to the respective sensor units reach the respective reagent systems at different times. Specifically, each of the sensor units has a controlling system between a sample supply inlet and the reagent system, and the controlling system controls the time it takes for the sample solution to reach the reagent system from the sample supply inlet.

Claims

1. A biosensor comprising:

a plurality of sensor units each comprising an electrode system including a working electrode and a counter electrode on an insulating base plate and a reagent system including an oxidoreductase and an electron mediator on or near the electrode system;

a sample supply inlet provided for each of the sensor units or for every some sensor units; and

a plurality of controlling systems for controlling the time it takes for a sample solution to reach the reagent system from the sample supply inlet, each controlling system being provided between the sample supply inlet and each of the reagent systems of the sensor units,

wherein said respective controlling systems are different from one another so that the sample solution supplied to the sample supply inlet reaches the respective reagent systems of the sensor units at different times; and

wherein said sample supply inlet is provided for each of the sensor units, and the biosensor has one working electrode terminal connected in parallel to the respective working electrodes of the sensor units and one counter electrode terminal connected in parallel to the respective counter electrodes of the sensor units.

2. A biosensor comprising:

a plurality of sensor units each comprising an electrode system including a working electrode and a counter electrode on an insulating base plate and a reagent system including an oxidoreductase and an electron mediator on or near the electrode system;

a sample supply inlet provided for each of the sensor units or for every some sensor units; and

a plurality of controlling systems for controlling the time it takes for a sample solution to reach the reagent system from the sample supply inlet, each controlling system being provided between the sample supply inlet and each of the reagent systems of the sensor units,

wherein said respective controlling systems are different from one another so that the sample solution supplied to the sample supply inlet reaches the respective reagent systems of the sensor units at different times; and

wherein said sample supply inlet is provided for each of the sensor units, and each of the sensor units further has a working electrode terminal connected to the working electrode and a counter electrode terminal connected to the counter electrode.

5. A method for determining the concentration of a specific substance contained in each of sample solutions supplied to an arbitrary number N of sensor units of the biosensor of claim 2,

which detects an electrochemical change of the electron mediator caused by a reaction between the specific substance in the sample solution and the oxidoreductase of the reagent system upon dissolution of the reagent system into the supplied sample solution after a lapse of a time period determined by said controlling system, said electrochemical change being detected by measuring a response current when a voltage is applied between the working electrode and the counter electrode,

said method comprising the steps of:

(a) supplying the sample solutions almost simultaneously to the respective sample supply inlets of the sensor units;

(b) applying a voltage between the working electrode terminal and the counter electrode terminal and measuring a response current N times at intervals determined by the respective controlling systems of the sensor units; and

(c) determining the concentration of the specific substance contained in each of the sample solutions supplied to the respective sensor units from the N measured values of the response current,

wherein the sample solutions supplied to the N sensor units are either the same sample solution or different sample solutions.

3. A biosensor comprising:

a plurality of sensor units each comprising an electrode system including a working electrode and a counter electrode on an insulating base plate and a reagent system including an oxidoreductase and an electron mediator on or near the electrode system;

a sample supply inlet provided for each of the sensor units or for every some sensor units; and

a plurality of controlling systems for controlling the time it takes for a sample solution to reach the reagent system from the sample supply inlet, each controlling system being provided between the sample supply inlet and each of the reagent systems of the sensor units,

wherein said respective controlling systems are different from one another so that the sample solution supplied to the sample supply inlet reaches the respective reagent systems of the sensor units at different times; and

wherein said controlling systems comprise a layer comprising a hydrophilic polymer, and said respective controlling systems of the senosr units are different from one another in the speed with which the layer comprising a hydrophilic polymer dissolves in the sample solution.

6. A method for determining the concentration of a specific substance contained in each of sample solutions supplied to an arbitrary number N of sensor units of the biosensor of claim 3,

which detects an electrochemical change of the electron mediator caused by a reaction between the specific substance in the sample solution and the oxidoreductase of the reagent system upon dissolution of the reagent system into the supplied sample solution after a lapse of a time period determined by said controlling system, said electrochemical change being detected by measuring a response current when a voltage is applied between the working electrode and the counter electrode,

said method comprising the steps of:

(a) supplying the sample solutions almost simultaneously to the respective sample supply inlets of the sensor units;

(b) applying a voltage between the working electrode terminal and the counter electrode terminal and measuring a current response sequentially unit by unit, a total of N times, at intervals determined by the respective controlling systems of the sensor units; and

(c) determining the concentration of the specific substance contained in each of the sample solutions supplied to the respective sensor units from the N measured values of the response current,

wherein the sample solutions supplied to the N sensor units are either the same sample solution or different sample solutions.

4. A biosensor comprising:

a plurality of sensor units each comprising an electrode system including a working electrode and a counter electrode on an insulating base plate and a reagent system including an oxidoreductase and an electron mediator on or near the electrode system;

a sample supply inlet provided for each of the sensor units or for every some sensor units; and

a plurality of controlling systems for controlling the time it takes for a sample solution to reach the reagent system from the sample supply inlet, each controlling system being provided between the sample supply inlet and each of the reagent systems of the sensor units,

wherein said respective controlling systems are different from one another so that the sample solution supplied to the sample supply inlet reaches the respective reagent systems of the sensor units at different times; and

wherein said controlling systems comprise a layer of a porous material or a fibrous substance having pores through which the sample solution passes, and said respective controlling systems of the sensor units are different from one another in the speed with which the sample solution passes through the layer pf the porous material or the fibrous substance.

7. A method for determining the concentration of a specific substance contained in a sample solution supplied to an arbitrary number N of sensor units of a biosensor comprising:

a plurality of sensor units each comprising an electrode system including a working electrode and a counter electrode on an insulating base plate and a reagent system including an oxidoreductase and an electron mediator on or near the electrode system;

a sample supply inlet provided for each of the sensor units or for every some sensor units; and

a plurality of controlling systems for controlling the time it takes for a sample solution to reach the reagent system from the sample supply inlet, each controlling system being provided between the sample supply inlet and each of the reagent systems of the sensor units,

wherein said sample supply inlet is shared by the respective sensor units, and the biosensor further has one working electrode terminal connected in parallel to the respective working electrodes of the sensor units and one counter electrode terminal connected in parallel to the respective counter electrodes of the sensor units and;

wherein said respective controlling systems are different from one another so that the sample solution supplied to the sample supply inlet reaches the respective reagent systems of the sensor units at different times

wherein said sample supply inlet is shared by the respective sensor units, and the biosensor further has one working electrode terminal connected in parallel to the respective working electrodes of the sensor units and one counter electrode terminal connected in parallel to the respective counter electrodes of the sensor units;

said method comprising the steps of;

(a) supplying the sample solution to the sample supply inlet;

(b) applying a voltage between the working electrode terminal and the counter electrode terminal and measuring a response current N times at intervals determined by the respective controlling systems of the sensor units; and

(c) determining the concentration of the specific substance contained in the sample solution supplied to the respective sensor units from the N measured values of the response current,

wherein the sample solution supplied to the N sensor units contains one or more specific substances, and the specific substances measured by the respective sensor units are the same or different.