Liquid dispense method and microarray manufacturing method

• US 7,323,224 B2
• Filed: 12/14/2005
• Issued: 01/29/2008
• Est. Priority Date: 12/22/2004
• Status: Expired due to Fees

• Current Assignee: Kazuhiko Ishihara, Seiko Epson Corporation (Seiko Group)
• Original Assignee: Kazuhiko Ishihara, Seiko Epson Corporation (Seiko Group)
• Inventors: Ishihara, Kazuhiko, Takagi, Fumio
• Primary Examiner(s): FLETCHER III, WILLIAM P
• Application Number:
  US11/300,647
• Publication Number:
  US 20060177585A1
• Time in Patent Office: 776 Days
• Field of Search: None
• US Class Current: 427/427.4
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01L 2300/161 Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries

Abstract

A method for discharging a liquid containing a biological material includes adding as a dispense stabilizer a polymer composed of phosphorylcholine-group-containing unsaturated compound units or a copolymer including same to the liquid containing the biological material; and discharging the liquid containing the dispense stabilizer using an inkjet system.

Claims

1. A method for discharging a liquid containing a biological material, comprising:

adding as a dispense stabilizer a polymer composed of phosphorylcholine-group-containing unsaturated compound units or a copolymer including same to the liquid containing the biological material; and

discharging the liquid containing the dispense stabilizer using an inkjet system.

2. The dispense method according to claim 1, wherein the copolymer includes as constituent units the phosphorylcholine-group-containing unsaturated compound units and (meth)acrylic ester units.

3. The dispense method according to claim 2, wherein the copolymer further includes as constituent units vinyl compound units other than the phosphorylcholine-group-containing unsaturated compound units and the(meth)acrylic ester units.

4. The dispense method according to claim 1, wherein the copolymer includes as constituent units the phosphorylcholine-group-containing unsaturated compound units, and vinyl compound units other than the phosphorylcholine-group-containing unsaturated compound units and (meth)acrylic ester units.

5. The dispense method according to claim 1, wherein the phosphorylcholine-group-containing unsaturated compound units are 2-methacryloyloxyethyl phosphorylcholine units.

6. The dispense method according to claim 2, wherein a molar ratio of the phosphorylcholine-group-containing unsaturated compound units to the(meth)acrylic ester units ranges from 100/0 to 30/10, excluding phosphorylcholine-group-containing unsaturated compound units)/(meth)acrylic ester units) being 100/0.

7. The dispense method according to claim 3, wherein the vinyl compound units other than phosphorylcholine-group-containing unsaturated compound units and (meth)acrylic ester units are contained in an amount ranging from 0 mole % to 95 mole %, excluding 0 mole %, relative to the total constituent units in the copolymer.

8. The dispense method according to claim 4, wherein a molar ratio of the phosphorylcholine-group-containing unsaturated compound units to the vinyl compound units other than phosphorylcholine-group-containing unsaturated compound units and (meth)acrylic ester units ranges from 100/0 to 5/95, excluding (phosphorylcholine-group-containing unsaturated compound units)/(vinyl compound units other than phosphorylcholine-group-containing unsaturated compound units and (meth)acrylic ester units) being 100/0.

9. The dispense method according to claim 1, wherein the dispense stabilizer is added to the liquid to achieve a concentration ranging from 0.005 to 500 mg/ml.

10. The dispense method according to claim 1, wherein the inkjet system is based on a piezoelectric drive system or an electrostatic drive system.

11. A microarray manufacturing method comprising:

discharging the liquid onto a solid substrate using the dispense method according to claim 1; and

immoibilizing the biological material contained in the liquid to the solid substrate.