Bio-barcodes based on oligonucleotide-modified particles

• US 7,323,309 B2
• Filed: 08/31/2005
• Issued: 01/29/2008
• Est. Priority Date: 03/28/2000
• Status: Expired due to Term

• Current Assignee: Northwestern University
• Original Assignee: Northwestern University
• Inventors: Nam, Jwa-Min, Park, So-Jung, Mirkin, Chad A.
• Primary Examiner(s): Riley; Jezia
• Application Number:
  US11/217,106
• Publication Number:
  US 20060051798A1
• Time in Patent Office: 881 Days
• Field of Search: 435/6, 536/23.1, 536/24.3
• US Class Current: 435/6.11
• CPC Class Codes: B01J 2219/00274 Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C07B 2200/11 Compounds covalently bound to a solid support
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 1/6827 for detection of mutation or polymorphism
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 2525/197 incorporating a spacer/coupling moiety
• CPC Class Codes: C12Q 2563/155 Particles of a defined size, e.g. nanoparticles
• CPC Class Codes: C12Q 2563/185 Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
• CPC Class Codes: C40B 40/00 Libraries per se, e.g. arrays, mixtures
• CPC Class Codes: Y10S 977/924 using nanostructure as support of dna analysis

Abstract

The present invention relates to a screening methods, compositions, and kits for detecting for the presence or absence of one or more target analytes, e.g. proteins such as antibodies, in a sample. In particular, the present invention relates to a method that utilizes reporter oligonucleotides as biochemical barcodes for detecting multiple protein structures or other target analytes in one solution.

Claims

1. A method for detecting for the presence of one or more target non-nucleic acid analytes in a sample comprising:

providing one or more types of particle complex probes, each probe comprising a particle having oligonucleotides bound thereto, a DNA barcode, and a oligonucleotide having bound thereto a specific binding complement to a target analyte, wherein (i) the DNA barcode has a sequence having at least two portions, (ii) at least some of the oligonucleotides attached to the particle have a sequence that is complementary to a first portion of the DNA barcode, (iii) the oligonucleotides having bound thereto a specific binding complement have a sequence that is complementary to a second portion of the DNA barcode, and (iv) the DNA barcode in each type of particle complex probe has a sequence that is different and that serves as an identifier for a particular target analyte;

wherein the DNA barcode is hybridized at least to some of the oligonucleotides attached to the particle and to the oligonucleotides having bound thereto the specific binding complement;

contacting the sample with a particle complex probe under conditions effective to allow specific binding interactions between the analyte and the particle complex probe and to form one or more aggregated complexes in the presence of analytes;

observing whether aggregate formation occurred;

isolating the aggregated complexes; and

analyzing the aggregated complexes by measuring melting temperature (Tm) of the aggregated complexes to determine presence of one or more target non-nucleic acid analytes.

2. The method of claim 1, wherein the target analyte is one or more antibodies and the specific binding complement is a hapten.

3. The method of claim 1, wherein the target analyte and the specific binding component are members of a specific binding pair.

4. The method of claim 3, wherein the specific binding pair comprises a ligand and receptor.

5. The method of claim 3, wherein the specific binding pair comprises an antibody and an antigen.

6. The method of claim 1, wherein the target analyte is a monovalent or a polyvalent ligand.

7. The method of claims 1 or 2, wherein the particles are nanoparticles.

8. The method of claims 1 or 2, wherein the particles are metal, semiconductor, insulator, or magnetic nanoparticles.

9. The method of claims 1 or 2, wherein the particles are gold nanoparticles.