Method for reducing effect of hematocrit on measurement of an analyte in whole blood
First Claim
1. A method for determining the presence and/or amount of an analyte in a sample of whole blood, the method comprising the steps of(a) providing a sample of whole blood for qualitative or quantitative determination of an analyte,(b) contacting the whole blood sample with a nonlytic hypertonic salt composition to adjust the hematocrit of the whole blood sample, and(c) detecting the presence and/or amount of analyte in the whole blood sample by measuring the analyte in the treated whole blood sample in the absence of a separation step.
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Abstract
A method for determining the presence and/or amount of an analyte in a sample of whole blood comprises the step of treating the sample with a nonlytic hypertonic salt composition to reduce the hematocrit by reducing the size of the red blood cells. In optical detection systems, the smaller red blood cells create greater scatter, which allows a more accurate correction to be applied in a dual-wavelength detection system. In electrochemical detection systems, as well as in optical detection systems, the smaller red blood cells provide less obstruction to the diffusion of analyte and reagents in the sample, to facilitate the reactions thereof.
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Citations
28 Claims
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1. A method for determining the presence and/or amount of an analyte in a sample of whole blood, the method comprising the steps of
(a) providing a sample of whole blood for qualitative or quantitative determination of an analyte, (b) contacting the whole blood sample with a nonlytic hypertonic salt composition to adjust the hematocrit of the whole blood sample, and (c) detecting the presence and/or amount of analyte in the whole blood sample by measuring the analyte in the treated whole blood sample in the absence of a separation step.
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17. A method for determining the presence and/or amount of an analyte in a sample of whole blood, the method comprising the steps of
(a) providing a sample of whole blood for qualitative or quantitative determination of an analyte, (b) contacting the whole blood sample with a nonlytic hypertonic salt composition prepared at at least 200 mM to adjust the hematocrit of the whole blood sample to adjust the hematocrit of the whole blood sample, and (c) detecting the presence and/or amount of analyte in the whole blood sample in the absence of a separation step.
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23. A method for determining the presence and/or amount of an analyte in a sample of whole blood, the method comprising the steps of
(a) providing a sample of whole blood for qualitative or quantitative determination of an analyte, (b) contacting the whole blood sample with a nonlytic hypertonic salt composition, and (c) detecting the presence and/or amount of analyte in the whole blood sample by measuring the analyte in the treated whole blood sample in the absence of a separation step.
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26. A method for determining the presence and/or amount of an analyte in a sample of whole blood, the method comprising the steps of
(a) providing a sample of whole blood for qualitative or quantitative determination of an analyte, (b) contacting the whole blood sample with a nonlytic hypertonic salt composition to modify the effect of the hematocrit of the whole blood sample, and (c) detecting the presence and/or amount of analyte in the whole blood sample by measuring the analyte in the treated whole blood sample in the absence of a separation step.
Specification