Raman spectroscopic monitoring of hemodialysis

• US 7,326,576 B2
• Filed: 04/09/2003
• Issued: 02/05/2008
• Est. Priority Date: 04/09/2003
• Status: Expired due to Fees

• Current Assignee: Prescient Medical, Inc.
• Original Assignee: Prescient Medical, Inc.
• Inventors: Clarke, Richard H., Womble, M. Edward
• Primary Examiner(s): Soderquist; Arlen
• Application Number:
  US10/410,051
• Publication Number:
  US 20040204634A1
• Time in Patent Office: 1,763 Days
• Field of Search: 250/339.07, 250/341.1, 250/339.12, 356/301, 356/303, 128/922, 128/925, 436/106, 436/108, 436/111, 436/164, 436/171, 600/310, 600/333, 600/366, 600/473, 600/475, 706/924, 702/19
• US Class Current: 436/108
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/14546 for measuring analytes not otherwise provided for, e.g. ions, cytochromes
• CPC Class Codes: A61M 1/14 Dialysis systems; Artificial kidneys; Blood oxygenators semi-permeable membranes characterised by the material, manufacturing processes therefor B01D71/00 ; Reciprocating systems for treatment of body fluids, e.g. single needle systems for hemofiltration or pheresis
• CPC Class Codes: A61M 1/3609 Physical characteristics of the blood, e.g. haematocrit, urea
• CPC Class Codes: A61M 1/367 Circuit parts not covered by the preceding subgroups of group A61M1/3621
• CPC Class Codes: A61M 2230/20 Blood composition characteristics
• CPC Class Codes: G01N 2021/651 Cuvettes therefore
• CPC Class Codes: G01N 21/65 Raman scattering
• CPC Class Codes: Y10T 436/17 Nitrogen containing
• CPC Class Codes: Y10T 436/171538 Urea or blood urea nitrogen
• CPC Class Codes: Y10T 436/173845 Amine and quaternary ammonium

Abstract

Spectroscopic systems and methods are disclosed for determining levels of at least one analyte in blood undergoing hemodialysis. In one aspect, the invention employs Raman spectroscopy to monitor and/or control hemodialysis. In one embodiment, the system uses a laser light directed to circulating blood from a patient undergoing dialysis to make Raman spectral measurements. For example, the laser light can be directed into a segment of the dialysis tubing. The system can utilize unique Raman spectroscopic signature of one or more analytes, e.g., urea, to identify and quantify such analytes against a whole blood background. Based on the spectral response, the concentration of the analytes can be monitored and/or used to control hemodialysis.

Claims

1. A method for monitoring hemodialysis, the method comprising the steps of:

adapting a Raman spectroscopy probe to a hemodialysis tube of a hemodialysis system;

irradiating a sample of blood in the tube that is undergoing hemodialysis by the system with light via the probe;

obtaining a Raman spectrum of chemical constituents from the sample via the probe; and

determining the concentration of urea in the sample based on a linear correlation between urea concentration and intensity of Raman scattered light at 1001 cm<sup>−

1</sup>.

2. The method of claim 1, further comprising:

repeating the steps of irradiating and analyzing to detect changes in level of the analyte over time.

3. The method of claim 1, wherein the step of irradiating the sample further comprises irradiating the sample with light of at least one wavelength in the range from about 700 nanometers to about 2.5 micrometers.

4. The method of claim 1, wherein the step of irradiating the sample further comprises irradiating the sample with light from a multi-mode laser.

5. The method of claim 1, wherein the step of irradiating the sample further comprises irradiating the sample with a broad band light source.

6. The method of claim 1, wherein the step of irradiating the sample further comprises irradiating the sample with infrared light.

7. The method of claim 1, wherein the step of obtaining a Raman spectrum of light scattered by the sample comprises detecting Raman scattered light using low resolution detector.

8. The method of claim 1, wherein the step of analyzing the spectrum further comprises determining the concentration of at least one analyte other than urea in the sample.

9. The method of claim 8, wherein the analyte other than urea comprises an analyte selected from a group consisting of:

a urea-based compound, an ammonium-based compound, a uric acid-based compound, and a nitrogen-based compound.

10. The method of claim 1, wherein the Raman spectroscopy probe comprises an optical head assembly that is operably connected by an optical fiber bundle to a light source and a light analyzer.

11. The method of claim 10, wherein the Raman spectroscopy probe further comprises a sampling tube connected to the optical head assembly and the step of adapting the Raman spectroscopy probe to the hemodialysis tube further comprises coupling the sampling tube to the hemodialysis tube using a coupler.

12. The method of claim 1, wherein the step of adapting the Raman spectroscopy probe to the hemodialysis tube comprises coupling the probe to the tube using a coupler.

13. The method of claim 12, wherein the Raman spectroscopy probe comprises an optical head assembly that is operably connected by an optical fiber bundle to a unit comprising a laser source and a light analyzer.

14. The method of claim 1, wherein the Raman spectroscopy probe comprises:

an optical head assembly that defines a light input pat and a light output path that are at least substantially orthogonal; and

a light redirecting element that redirects light from the input path toward the sample and transmits light from the sample to the output path.

15. The method of claim 14, wherein the light directing element comprises a dichroic mirror.

16. A method for monitoring a subject'"'"'s urea concenintion, the method comprising the steps of:

irradiating a blood sample from a subject using a Raman spectroscopic probe comprising;

an optical head assembly that defines a light input path and a light output path that are at least substantially orthogonal, and

a light redirecting element that redirects light from the input path toward the sample and transmits light from the sample to the output path;

collecting a Raman spectrum of light scattered by the sample; and

analyzing the intensity of the Raman spectrum at 1001 cm<sup>−

1</sup> to detect the concentration of urea in the sample using a linear correlation between intensity of the Raman spectrum at 1001 cm<sup>−

1</sup> and the concentration of urea, the concentration of urea being indicative of the progress of dialysis or kidney function.

17. The method of claim 16, further comprising:

repeating the steps of irradiating and analyzing to detect changes in urea concentration over time.

18. The method of claim 16, wherein the decrease in concentration of urea is indicative of elimination of toxins from the blood sample during dialysis.

19. The method of claim 16, wherein the Raman spectrum is analyzed by a low resolution Raman spectroscopy system.

20. The method of claim 16, wherein the sample is present within an at least substantially transparent tube.

21. The method of claim 20, further comprising the step of coupling the probe to the tube using a coupler.

22. The method of claim 16, wherein the light directing element comprises a dichroic mirror.

23. A hemodialysis system comprising:

at least one blood cleaning element for removing toxins from blood;

hemodialysis tubing;

a light source to irradiate a sample of blood in the hemodialysis tubing that is undergoing hemodialysis;

a Raman spectrometer to detect Raman scattered light of at least one analyte in the sample including urea, the concentration of the analyte being indicative of the progress of hemodialysis; and

a Raman spectroscopy probe mediating optical communication between the light source and the sample of blood in the hemodialysis tubing and between the Raman spectrometer and the sample of blood in the hemodialysis tubing,

wherein the system is configured to determine urea concentration in the sample based on a linear correlation between urea concentration and intensity of Raman scattered light at 1001 cm<sup>−

1</sup>.

24. The system of claim 23, wherein the system further comprises a processor that controls hemodialysis based, at least in part, on data from the Raman spectrometer.

25. The system of claim 23, further comprising a probe coupler that couples the probe to the tubing.

26. The method of claim 25, wherein the Raman spectroscopy probe comprises an optical head assembly that is operably connected by an optical fiber bundle to a light source and a light analyzer.

27. The method of claim 23, wherein the Raman spectroscopy probe comprises an optical head assembly that is operably connected by an optical fiber bundle to a light source and a light analyzer.

28. The method of claim 27, wherein the Raman spectroscopy probe further comprises a sampling tube connected to the optical head assembly and the probe is coupled to the hemodialysis tubing via the sampling tube.

29. The method of claim 23, wherein the Raman spectroscopy probe comprises:

an optical head assembly that defines a light input path and a light output path that are at least substantially orthogonal; and

a light redirecting element that redirects light from the input path toward the sample and transmits light from the sample to the output path.

30. The method of claim 29, wherein the light directing element comprises a dichroic mirror.