Method and apparatus for detecting biomolecular interactions
First Claim
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1. An instrument for detecting light reflected from a surface of a biosensor, said instrument comprising:
- a light source;
an illuminating fiber optically coupled to said light source, said illuminating fiber illuminating an area of said surface of said biosensor;
a detecting fiber for detecting radiation reflected from said surface;
a spectrometer receiving said reflected radiation; and
wherein the instrument is further configured for determining a peak wavelength value of said reflected radiation; and
wherein said biosensor comprises;
a grating comprised of a material having a high refractive index, said grating having a depth and a period; and
a substrate layer that supports said grating;
wherein, when said biosensor is illuminated with light from said light source a resonant grating effect is produced, and wherein said depth of and period of said grating are less than the wavelength of the resonant grating effect.
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Abstract
Method and apparatus for detecting biomolecular interactions. The use of labels is not required and the methods may be performed in a high-throughput manner. An apparatus for detecting biochemical interactions occurring on the surface of a biosensor includes a light source. A first optical fiber is coupled to the light source and illuminates the biosensor. A second optical fiber detects a wavelength reflected from the biosensor. A spectrometer determines spectra of a reflected signal from the biosensor.
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Citations
7 Claims
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1. An instrument for detecting light reflected from a surface of a biosensor, said instrument comprising:
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a light source; an illuminating fiber optically coupled to said light source, said illuminating fiber illuminating an area of said surface of said biosensor; a detecting fiber for detecting radiation reflected from said surface; a spectrometer receiving said reflected radiation; and wherein the instrument is further configured for determining a peak wavelength value of said reflected radiation; and
wherein said biosensor comprises;a grating comprised of a material having a high refractive index, said grating having a depth and a period; and a substrate layer that supports said grating; wherein, when said biosensor is illuminated with light from said light source a resonant grating effect is produced, and wherein said depth of and period of said grating are less than the wavelength of the resonant grating effect. - View Dependent Claims (2, 3, 4, 5)
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6. An instrument for detecting light reflected from a surface of a biosensor, said instrument comprising:
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a white light source; an illuminating fiber optically coupled to said light source, said illuminating fiber illuminating an area of said surface of said biosensor; a detecting fiber for detecting radiation reflected from said surface; a spectrometer receiving said reflected radiation; and wherein the instrument is further configured for determining a peak wavelength value of said reflected radiation; wherein the illuminating fiber and the detecting fiber are provided in a unitary optical fiber probe; and wherein said optical fiber probe includes a collimating lens for collimating white light from said light source.
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7. An instrument for detecting light reflected from a surface of a biosensor, said instrument comprising:
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a light source; an illuminating fiber optically coupled to said light source, said illuminating fiber illuminating an area of said surface of said biosensor; a detecting fiber for detecting radiation reflected from said surface; a spectrometer receiving said reflected radiation; and wherein the instrument is further configured for determining a peak wavelength value of said reflected radiation; and an incubator that incubates said biosensor at a predetermined temperature.
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Specification