Retentate chromatography and protein chip arrays with applications in biology and medicine
First Claim
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1. A method for preparing a substrate for detecting at least one analyte in a sample comprising the steps of:
- a) exposing the sample to at least two different selectivity conditions, each selectivity condition defined by the combination of an adsorbent and an eluant, to allow retention of the analyte by the adsorbent;
b) identifying by desorption spectrometry at least one selectivity condition under which the analyte is retained; and
c) immobilizing at least one adsorbent of an identified selectivity condition on the substrate, whereby the substrate for detecting at least one analyte is prepared.
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Abstract
This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.
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Citations
23 Claims
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1. A method for preparing a substrate for detecting at least one analyte in a sample comprising the steps of:
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a) exposing the sample to at least two different selectivity conditions, each selectivity condition defined by the combination of an adsorbent and an eluant, to allow retention of the analyte by the adsorbent; b) identifying by desorption spectrometry at least one selectivity condition under which the analyte is retained; and c) immobilizing at least one adsorbent of an identified selectivity condition on the substrate, whereby the substrate for detecting at least one analyte is prepared. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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